Magnesium chloride solution

The ROCK kinase assay is a luminescent kinase assay that measures IOP formed from a kinase reaction. ADP is converted into ATP and is converted into light by Kinase-Glo Luciferase. The Kinase-Glo Assay can be used to monitor the activity of virtually any ADP-generating enzyme. The relative light unit (RLU) without test compound was set as 100% (blank value), and that without enzyme and compound was set as 0% (normal value). The reaction rate (% of black) was then calculated from the RLU.
The following reagents were used in this assay: ROCK1 (750 ug/mL, Carna Biosciences, Cat.01-109); long S6 kinase substrate peptide (Millipore, Cat#12-420); magnesium chloride solution (Sigma, 60142-100 mL); EGTA solution (0.5 M pH 8.0, Bioword, Cat. 4052008-1); Trizma^® hydrochloride solution (1 M, pH 7.5 Sigma, T2319-100 mL); bovine serum albumin solution (20 mg/mL in water, Sigma); Kinase-Glo luminescent kinase assay (Promega, RV6712); ripasudil (K115) hydrochloride dehydrate (Selleckchem, S7995-5 mg); ATP solution (100 mM, GE Healthcare, Cat.27-2056-010). A ROCK kinase inhibitor k-115 was used as the positive control.
$$\mathrm{Inhibition} (\%)=\frac{100 –\{\left(\mathrm{activity} \mathrm{of} \mathrm{Enzyme} \mathrm{with} \mathrm{Test} \mathrm{Cmpd} \& \mathrm{Sbustrate}-\mathrm{Min}\right)\}\times 100}{\mathrm{Max}-\mathrm{Min}}$$
Max = observed enzyme activity measured in the presence of enzyme, substrate(s), and cofactors utilized in the method.
Min = Normal value in the method

The active substances contained in these products belong to various chemical groups, such as alcohols, quaternary ammonium compounds, oxidizing compounds, and glucoprotamine. In total, 12 commercial products for disinfection were selected for the study, including 6 products in the form of a liquid ready for soaking wipes or in the form of a concentrate for the preparation of working solutions and 6 products in the form of commercial wipes soaked in a disinfectant (Table 1).
Products in the form of concentrates (No. 1, 2, and 4) were diluted in hard water containing 6 mL/L of solution A (19.84 g/L of magnesium chloride solution (Sigma) and 46.24 g/L of calcium chloride (Chempur, Karlsruhe, Germany)) and 8 mL/L of solution B (35.02 g/L of sodium bicarbonate (Chempur)).
All commercial wipes still had a valid shelf life, and their original packaging was opened just prior to testing. Self-made wipes soaked in disinfectant according to EN 16615 were used just after preparation.