Specific rotations and IR (KBr) data were measured on a PerkinElmer model 341 polarimeter (Perkin-Elmer Inc., Waltham, MA, USA) and Jasco FTIR400 spectrometer (Jasco Inc., Tokyo, Japan), respectively. CD and UV spectra were obtained on a Jasco J-715 spectropolarimeter (Jasco Inc., Tokyo, Japan) and UV-8000 spectrophotometer (Shanghai Metash instruments Co., Shanghai, China) in MeOH, respectively. 1D and 2D NMR spectra were acquired using a Bruker AMX-500 instrument (500 MHz for 1H NMR, 125 MHz for 13C NMR) (Bruker Biospin Corp., Billerica, MA, USA) at room temperature. HRESIMS data were measured on an Agilent 6210 LC/MSD TOF mass spectrometer (Agilent Technologies Inc. Lake Forest, CA, USA). HPLC separation was performed using a YMC-Pack Pro C18 (5 μm) column (YMC Co. Ltd., Kyoto, Japan) using a Waters 1525 separation module equipped with a Waters 996 Photodiode Array (PDA) detector (Waters Corp., Milford, MA, USA). Column chromatographic purifications were performed on silica gel 60 (200–300 mesh, Qingdao Ocean Chemical Co., Qingdao, China), ODS (50 μm, YMC Co. Ltd., Kyoto, Japan), and Sephadex LH-20 (Pharmacia Co., Piscataway, NJ, USA).
Waters 1525 separation module
Manufactured by Waters Corporation
Sourced in United States
The Waters 1525 separation module is a laboratory instrument designed for liquid chromatography. It provides precise control of mobile phase flow rates and gradients, enabling the separation and isolation of chemical compounds within a sample. The module's core function is to deliver the mobile phase to the analytical column in a controlled and consistent manner, which is essential for accurate and reproducible chromatographic separations.
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Lab products found in correlation
Amx 500 instrument, by Bruker (2 mentions)
Agilent 6210 lc msd tof mass spectrometer, by Agilent Technologies (2 mentions)
Uv 8000 spectrophotometer, by Metash (2 mentions)
Waters 996 photodiode array pda detector, by Waters Corporation (1 mentions)
Model 341 polarimeter, by PerkinElmer (1 mentions)
J 715 spectropolarimeter, by Jasco (1 mentions)
Acquity qda detector, by Waters Corporation (1 mentions)
Waters 2998 photodiode array detector, by Waters Corporation (1 mentions)
Xbridge prep c18, by Waters Corporation (1 mentions)
Xevo g2 xs qtof mass spectrometer, by Waters Corporation (1 mentions)
Octadecylsilyl silica gel, by YMC (1 mentions)
Pack pro c18 rs, by YMC (1 mentions)
Amx 500, by Bruker (1 mentions)
Avance 600, by Bruker (1 mentions)
Vertex 70v ftir spectrometer, by Bruker (1 mentions)
Waters 2996 photodiode array detector, by Waters Corporation (1 mentions)
4 protocols using waters 1525 separation module
1
Spectroscopic Characterization of Compounds
2
Characterization of Organic Compounds
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Optical rotations were measured with an Autopol VI, Serial #91003, and UV spectra on a Persee Tu-1,950 spectrophotometer. NMR spectra were obtained from an Agilent DD2 600 MHz NMR spectrometer and a Brucker AVANCE III HD 600 MHz NMR spectrometer. The ESI-MS analysis was performed on a Waters purification system coupled with a Waters Acquity QDa detector. The High-resolution TOF-ESI-MS spectra were acquired with a Waters XeVO G2-XS Q-TOF mass spectrometer. Preparative HPLC and semi-preparative HPLC were performed, respectively, on YMC-Pack Pro C18 RS (20 × 250 mm, 5 μm) column and Waters Xbridge Prep C18 (10 × 250 mm, 5 μm) column, coupled with a Waters 1,525 separation module and a Waters 2,998 photodiode array detector.
3
Instrumentation for Spectroscopic Analysis
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Optical rotations were obtained on the Shanghai Shenguang SGWzz-2 model automatic polarimeter (Shanghai Shenguang Instrument and Meter Co., Ltd., Shanghai, China). IR spectra of all compounds were recorded on Bruker’s VERTEX 70v FT-IR Spectrometer (Bruker Biospin Corp., Billerica, Mass., USA). UV and CD spectra were measured on a JASCO-810 model spectrometer (Jasco Inc., Tokyo, Japan). HRESIMS data were recorded on an Agilent 6,520 AccuTOF LC-plus 4G instrument (JEOL., Tokyo, Japan). The 1D and 2D spectral data were acquired on Bruker AMX-500 and Bruker AVANCE-600 instruments (Bruker Biospin Corp., Billerica, Mass., USA). Semi-performance liquid chromatography was performed on a Waters 1,525 separation module (Waters Corp., Milford, Mass., USA), with YMC-PackPro C18 RS (5 μm) columns and octadecyl silyl silica gel (50 μm, YMC Co. Ltd., Kyoto, Japan). Column chromatographic purifications were performed on silica gel 60 (200–300 mesh, Qingdao Ocean Chemical Co., Qingdao, China), ODS (50 μm, YMC Co. Ltd., Kyoto, Japan).
4
Comprehensive Characterization of Natural Compounds
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Nuclear magnetic resonance (NMR) spectra were measured on a Bruker AMX-500 instrument (Bruker Biospin Corp., Billerica, MA, USA) at 500 MHz for 1H-NMR and 125 MHz for 13C-NMR. High-resolution electrospray ionization mass spectroscopy (HRESIMS) data were obtained on an Agilent 6210 LC/MSD TOF mass spectrometer (Agilent Technologies Inc. Lake Forest, CA, USA), and ultraviolet (UV) spectra were obtained on a UV-8000 spectrophotometer (Shanghai Metash instruments Co., Shanghai, China). Optical rotations were measured on an Anton Paar MCP 5500 (Anton Paar Co, Graz, Austria). Semi-preparative high-performance liquid chromatography (HPLC) was performed on a Waters 1525 separation module (Waters Corp., Milford, MA, USA) equipped with a Waters 2996 photodiode array detector (Waters Corp., Milford, MA, USA) by using YMC-Pack Pro C18 RS (5 µm) columns (YMC Co. Ltd., Kyoto, Japan). Purifications were conducted by Agilent LC1263 Infinity Ⅱ (Agilent Technologies Inc., CA, USA). Thin-layer chromatography analysis was run on HSGF254-precoated silica gel plates (10–40 mm, Yantai Chemical Plant, Yantai, China).
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