Corona plate reader sh 9000

The viability of human myeloma cell lines was measured using the Cell Counting Kit-8 (CCK-8) from Dojindo (Tokyo, Japan). These cells were seeded in a 96-well plate at a density of 2 × 10⁴ cells per well. The cells were then treated, as indicated, with each reagent. CCK-8 solution (10 μL) was added to each well, and the plates were incubated for an additional 2 h at 37 °C. Optical densities at 450 and 650 nm were measured using a Corona plate reader SH-9000 (Hitachi, Tokyo, Japan). The viability of primary myeloma cells was assessed using the CellTiter-Glo^® 2.0 Cell Viability Assay Kit (Promega Corporation, Madison, WI, USA) according to the supplier’s protocol and analyzed using a Corona plate reader SH-9000 (Hitachi, Tokyo, Japan).

The absorbance of carbapenems, including imipenem (Wako Pure Chemical Industries, Osaka, Japan), meropenem (Tokyo Chemical Industry, Tokyo, Japan), doripenem, biapenem, and ertapenem (Merck, Kenilworth, NJ, USA), was measured using a Corona plate reader SH-9000 (Hitachi, Tokyo, Japan) at room temperature (approximately 25 °C) in a UV-transparent 96-well plate (Zell-kontakt, Nörten-Hardenberg, Germany). Measurements with bandwidths of 1 nm and 5 nm were implemented for analysing absorption spectra.

The CCK-8 from Dojindo (Tokyo, Japan) was used to measure the cell viability. IM-9 cells were seeded into a 96-well plate, with a density of 8 × 10³ cells/well. Then, the cells were treated as indicated with each drug for 24 or 72 h. Briefly, we added 10 μL CCK-8 solution to each well, and the plates were incubated for an additional 2 h at 37 °C. The optical densities at 450 and 650 nm were measured in a Corona plate reader SH-9000 (Hitachi, Tokyo, Japan). Then, the cell viability of the IM-9 cells was further confirmed using the trypan blue dye exclusion assay. The cells (2 × 10⁵ cells/mL) were inoculated into a 12-well plate and administered vehicle (DMSO) or KIRA8 (0.1 to 10 μM) for 60 min (min) followed by bortezomib (5 nM or 10 nM) or thapsigargin (500 nM) for 24 h, respectively. Next, the cells were stained with 0.4% trypan blue solution (Gibco, Grand Island, NY, USA), and the viable cells were counted using a hemocytometer counting chamber (Burker-turk, Erma Inc, Tokyo, Japan).