Cytokine monkey magnetic 29 plex panel

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Cytokine Monkey Magnetic 29-Plex Panel is a lab equipment product designed for the simultaneous measurement of 29 different cytokines in monkey biological samples. It uses magnetic bead-based multiplex technology to enable the quantitative analysis of multiple analytes in a single well. The panel can be used for research purposes in the field of immunology and inflammation.

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Lab products found in correlation

Bio plex 200 system, by Bio-Rad (3 mentions) Luminex 200, by DiaSorin (3 mentions) Bio plex manager software v 6, by Bio-Rad (2 mentions) Luminex xponent, by Thermo Fisher Scientific (2 mentions) Luminex 200, by Thermo Fisher Scientific (2 mentions) Blood ammonia assay kit, by Nanjing Jiancheng (1 mentions) Au5800 series, by Beckman Coulter (1 mentions) Luminex 100 instrument, by Thermo Fisher Scientific (1 mentions) Mqx200 microplate reader, by Agilent Technologies (1 mentions) Luminex 200 system, by Merck Group (1 mentions) Human elisa max deluxe set, by BioLegend (1 mentions) Luminex 200 machine, by DiaSorin (1 mentions) Magpix system, by DiaSorin (1 mentions) Elisa, by R&D Systems (1 mentions)

17 protocols using cytokine monkey magnetic 29 plex panel

Cytokine and Chemokine Profiling in Infection

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Systemic changes in chemokines and cytokines were measured using an Invitrogen Monkey Cytokine Magnetic 29-Plex Panel (Invitrogen, Carlsbad, CA), as per the manufacturer’s instructions using a Bio-Rad Bio-Plex Pro II Wash Station (Bio-Rad, Hercules, CA) and the Bio-Rad Bio-Plex 200 System (Bio-Rad) [69 (link),74 (link)]. Plasma samples taken from both pre- and postinfection were assayed and used to calculate the total fold change in the plasma chemokine and cytokine levels. These fold change values were then normalized using a log₂ transformation. The transformed data was used to generate a heatmap via the publicly available Morpheus software from the Broad Institute (https://software.broadinstitute.org/morpheus/)

Raehtz K.D., Barrenäs F., Xu C., Busman-Sahay K., Valentine A., Law L., Ma D., Policicchio B.B., Wijewardana V., Brocca-Cofano E., Trichel A., Gale M J.r., Keele B.F., Estes J.D., Apetrei C, & Pandrea I. (2020). African green monkeys avoid SIV disease progression by preventing intestinal dysfunction and maintaining mucosal barrier integrity. PLoS Pathogens, 16(3), e1008333.

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Multiplex Cytokine Profiling in MCM

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Luminex technology was used to assess plasma chemokine and cytokine levels following NKTT320 treatment in 12 MCM. We used the Invitrogen (Carlsbad, CA) magnetic bead Monkey Cytokine Magnetic 29-Plex Panel covering the following analytes: EGF, HGF, VEGF, MIG, RANTES, Eotaxin, IL-8, GM-CSF, TNF alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, MIP-1 alpha, IL-17, MIP-1 beta, IP-10, IL-15, MCP-1, G-CSF, IFN gamma, FGF-Basic, IL-1RA, MDC, MIF, I-TAC. Final reactions were read on a Bio-Plex® 200 System (Bio-Rad Laboratories, Hercules, CA) results were calculated using Bio-Plex Manager™ Software v6.2 (Bio-Rad) by the TNPRC Pathogen Quantification and Detection Core.

Bond N.G., Fahlberg M.D., Yu S., Rout N., Tran D., Fitzpatrick-Schmidt T., Sprehe L.M., Scheef E.A., Mudd J.C., Schaub R, & Kaur A. (2022). Immunomodulatory potential of in vivo natural killer T (NKT) activation by NKTT320 in Mauritian-origin cynomolgus macaques. iScience, 25(3), 103889.

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Multimodal Monitoring in ALF Induction

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After ALF induction, all monkeys were monitored every 12 h for the first 48 h and every 24 h for the remainder of the study. During the 6 h treatment, animals were monitored every hour (Figure 1B). Vital signs were recorded by cardiogram monitoring. Blood samples were collected for subsequent analyses. Serum biochemistry was assayed using a chemistry analyzer AU5800 series (Beckman Coulter). Ammonia concentrations were quantified by a blood ammonia assay kit (Nanjing Jiancheng, Nanjing, China). S-100 β proteins have emerged as a biomarker of blood-brain barrier (BBB) permeability and neuropathological conditions including encephaledema and increased ICP 13 (link), 14 (link). Thus, elevated S-100 β levels were measured using an ELISA kit (Ruikesi, Chengdu, China). Rhesus IgG and IgM levels were detected using ELISA kits (Ruikesi). All the kits were analyzed with a MQX 200 microplate reader (BioTek Instruments Inc., VT, US). Cytokines were assessed using a Luminex 100 instrument with xPONENT 3.1 software using Monkey Cytokine Magnetic 29-Plex Panel (Invitrogen, CA, US).

Li Y., Wu Q., Wang Y., Weng C., He Y., Gao M., Yang G., Li L., Chen F., Shi Y., Amiot B.P., Nyberg S.L., Bao J, & Bu H. (2018). Novel spheroid reservoir bioartificial liver improves survival of nonhuman primates in a toxin-induced model of acute liver failure. Theranostics, 8(20), 5562-5574.

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Multiplex Cytokine Profiling in Macaques

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Bond N.G., Fahlberg M., Yu S., Rout N., Tran D., Fitzpatrick‐Schmidt T., Sprehe L.M., Scheef E.A., Mudd J.C., Schaub R.G., & Kaur A. (2021). Adjuvant and immunomodulatory potential of in vivo Natural Killer T (NKT) activation by NKTT320.

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Evaluating ZIKV Infection in Rhesus Monkeys

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Rhesus monkeys (Macacamulatta) were administered with EtOH or 25HC intravenously (i.v.) at 1.5 mg/kg of body weight 24 hr pre- or 4 hr post- intramuscular (i.m.) infection with 1×10⁵ or 1×10³ PFU of ZIKV GZ01 strain. They were subsequently treated with 25HC or EtOH daily for 7 days, and their body temperature and clinical signs were monitored daily. The blood and urine samples were collected from 0–7 dpi for viral load analysis by qRT-PCR. The serum collected at 0, 3, and 7 dpi were subjected to cytokine analysis with Monkey Cytokine Magnetic 29-Plex Panel (Thermo Fisher, LPC0005M) according to the manufacturer’s instructions. The data were collected on Luminex200 and analyzed by Luminex xPONENT (Thermo Fisher).

Li C., Deng Y.Q., Wang S., Ma F., Aliyari R., Huang X.Y., Zhang N.N., Watanabe M., Dong H.L., Liu P., Li X.F., Ye Q., Tian M., Hong S., Fan J., Zhao H., Li L., Vishlaghi N., Buth J.E., Au C., Liu Y., Lu N., Du P., Qin F.X., Zhang B., Gong D., Dai X., Sun R., Novitch B.G., Xu Z., Qin C.F, & Cheng G. (2017). 25-Hydroxycholesterol Protects Host against Zika Virus Infection and Its Associated Microcephaly in a Mouse Model. Immunity, 46(3), 446-456.

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Cytokine and Protein Profiling in Irradiated Monkeys

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Plasma and BAL supernatant samples from irradiated monkeys were assessed for 29 cytokines using the Monkey Cytokine Magnetic 29-Plex Panel (LPC0005M, Thermo Fisher); for TGF-β1, TGF-β2, and TGF-β3 using the U-PLEX TGF-β Combo NHP kit (catalog # K15243K, Meso Scale Diagnostics LLC) and Meso QuickPlex SQ120 (Meso Scale Diagnostics LLC); and for SERPINA3 using the Monkey Alpha1 Antichymotrypsin ELISA Kit (MBS7209859, MyBioSource) according to the manufacturers’ instructions (additional details are provided in Methods E1).

Thakur P., DeBo R., Dugan G.O., Bourland J.D., Michalson K.T., Olson J.D., Register T.C., Kock N.D, & Cline J.M. (2021). Clinicopathologic and Transcriptomic Analysis of Radiation-Induced Lung Injury in Nonhuman Primates. International journal of radiation oncology, biology, physics, 111(1), 249-259.

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Multiplex Cytokine Profiling of Frozen Plasma

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Plasma was frozen at −80°C immediately during blood processing, then batch tested as per kit instructions using the Monkey Cytokine Magnetic 29-Plex Panel (ThermoFisher) for the following cytokines, chemokines and growth factors: GM-CSF, TNF, IL-1β, IL-4, IL-6, MIG⁸, VEGF⁹, HGF¹⁰, EGF¹¹, IL-8, IL-17, MIP-1α, IL-12, IL-10, FGF-Basic¹², IFN-γ, G-CSF, MCP-1¹³, IL-15, IP-10¹⁴, MIP-1β, Eotaxin, RANTES, IL-1RA¹⁵, I-TAC¹⁶, MDC¹⁷, IL-5, IL-2, and MIF¹⁸.

Dross S.E., Munson P.V., Kim S.E., Bratt D.L., Tunggal H.C., Gervassi A.L., Fuller D.H, & Horton H. (2016). Kinetics of myeloid derived suppressor cell frequency and function during SIV infection, combination antiretroviral therapy and treatment interruption. Journal of immunology (Baltimore, Md. : 1950), 198(2), 757-766.

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Cytokine Profiling of Monkey Sera

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Monkey sera collected at different time points were subjected for cytokine, chemokines, and growth factors analysis with the Monkey Cytokine Magnetic 29-Plex Panel (Thermo Fisher, LPC0005M, USA) according to the manufacturer’s instruction. Each serum sample was measured with three replicates. The data were collected on Luminex200 and analyzed by Luminex xPONENT (Thermo Fisher, USA).

Deng Y.Q., Zhang N.N., Li X.F., Wang Y.Q., Tian M., Qiu Y.F., Fan J.W., Hao J.N., Huang X.Y., Dong H.L., Fan H., Wang Y.G., Zhang F.C., Tong Y.G., Xu Z, & Qin C.F. (2017). Intranasal infection and contact transmission of Zika virus in guinea pigs. Nature Communications, 8, 1648.

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Plasma Cytokine Profiling in Dietary Interventions

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Plasma samples from the following time points: before WSD, 4 months on a WSD, and 4 months on caloric restriction, (stored at −80 °C) were thawed and analyzed in duplicates using the Invitrogen Cytokine Monkey Magnetic 29-Plex Panel per the manufacturer’s instructions, using the Magpix spectrophotometer (Life Technologies, Grand Island, NY). The panel includes monocyte chemoattractant protein 1 (MCP-1; CCL2), fibroblast growth factor basic (FGF-β), IL-1β, granulocyte colony-stimulating factor (G-CSF), IL-10, IL-6, IL-12, RANTES, eotaxin, IL-17, macrophage inflammatory protein 1 alpha (MIP-1α), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage inflammatory protein 1 beta (MIP-1β), IL-15, epidermal growth factor (EGF), IL-5, hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), IFN-γ, monocyte-derived chemokine (MDC; CCL22), interferon-inducible T cell alpha chemoattractant (ITAC; CXCL11), migration inhibition factor (MIF), IL-1 receptor agonist (IL-1RA), TNF-α, IL-2, IFN-gamma-inducible protein 10 (IP-10, CXCL10) monokine induced by IFN-gamma (MIG; CXCL9), IL-4, and IL-8 (see Additional file 1: Figure S1 for details).

Messaoudi I., Handu M., Rais M., Sureshchandra S., Park B.S., Fei S.S., Wright H., White A.E., Jain R., Cameron J.L., Winters-Stone K.M, & Varlamov O. (2017). Long-lasting effect of obesity on skeletal muscle transcriptome. BMC Genomics, 18, 411.

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Multiplex Analysis of Monkey Plasma Cytokines

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Plasma samples were thawed and analyzed in duplicates using the
Invitrogen Cytokine Monkey Magnetic 29-Plex Panel per the manufacturer’s
instructions (Life Technologies, Grand Island, NY). The panel includes monocyte
chemoattractant protein 1 (MCP-1; CCL2), fibroblast growth factor basic (FGF-b),
IL-1β, granulocyte colony-stimulating factor (G-CSF), IL-10, IL-6,
IL-12, RANTES, eotaxin, IL-17, macrophage inflammatory protein 1 alpha
(MIP-1α), granulocyte-macrophage colony-stimulating factor (GM-CSF),
macrophage inflammatory protein 1 beta (MIP-1β), IL-15, epidermal growth
factor (EGF), IL-5, hepatocyte growth factor (HGF), vascular endothelial growth
factor (VEGF), IFN-γ, monocyte-derived chemokine (MDC; CCL22),
interferon-inducible T cell alpha chemoattractant (ITAC; CXCL11), migration
inhibition factor (MIF), IL-1 receptor agonist (IL-1RA), TNF-α, IL-2,
IFN-gamma-inducible protein 10 (IP-10, CXCL10) monokine induced by IFN-gamma
(MIG; CXCL9), IL-4, and IL-8. IL-6 and MDC showed the significant differences
between intact and orchidectomized groups and were included in the
“Results” section.

Cameron J.L., Jain R., Rais M., White A.E., Beer T.M., Kievit P., Winters-Stone K., Messaoudi I, & Varlamov O. (2016). Perpetuating Effects of Androgen Deficiency on Insulin-resistance. International journal of obesity (2005), 40(12), 1856-1863.

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