Eight-week in vivo implants were fixed in formalin and stored in PBS. Micro-computed tomography (micro-CT) data were acquired from the implants using a phoenix nanotom m scanner (General Electric) with 0.5-mm aluminum filtered x-rays (applied voltage 70 kV; current 260 mA). Transmission images were acquired during a 360° scan rotation with an incremental rotation step size of 0.25°. Reconstruction was performed using a modified Feldkamp algorithm at an isotropic voxel size of 25 µm. Threshold-based segmentation and 3D measurement analyses (bone mineral density and volume) were performed using ImageJ software54 (link) with the BoneJ55 (link) and 3D Shape56 (link) extensions. For quantification of bone mineral density and volume, all data were normalized to the size of each construct. Three-dimensional rendering of the structures was performed using VGStudio MAX 2.2 software (Volume Graphics).
Phoenix nanotom m scanner
Manufactured by GE Healthcare
The Phoenix nanotom m scanner is a high-resolution computed tomography (CT) system designed for non-destructive testing and analysis. It features a high-performance X-ray tube and a powerful detector that enable the acquisition of high-quality 3D images of a wide range of samples.
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Lab products found in correlation
2 protocols using phoenix nanotom m scanner
1
Micro-CT Analysis of In Vivo Implants
2
Micro-CT Analysis of Bone Mineralization
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After explantation, constructs were fixed in 4% paraformaldehyde overnight and stored in PBS until micro-computed tomography (μ-CT) analysis. Data were acquired by using a phoenix nanotom m scanner (General Electric, Fairfield, CT) with 0.5-mm aluminum filtered x-rays (applied voltage 70 kV; current 260 μA). Transmission images were acquired during a 360° scan rotation with an incremental rotation step size of 0.25°. Reconstruction was made using a modified Feldkamp algorithm at an isotropic voxel size of 2.5 μm. Threshold-based segmentation and 3D measurement analyses (bone mineral density and volume) were performed using ImageJ software (Schneider et al., 2012 (link)) with the BoneJ (Doube et al., 2010 (link)) and 3D Shape (Sheets et al., 2013 (link)) extensions. The threshold employed for the segmentation was set at 325 mg/cm3, as previously determined to clearly distinguish between ceramic and newly formed mineralized tissue (Scheufler et al., 2008 (link)). Three-dimensional rendering of the structures was performed using VGStudio MAX 2.2 software (Volume Graphics, Heidelberg, Germany).
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