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Caerulein cae

Manufactured by Merck Group
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Caerulein (Cae) is a laboratory product offered by Merck Group. It is a peptide that serves as a core function in various research applications.

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Lab products found in correlation

Recombinant mouse il 4, by R&D Systems (1 mentions) Recombinant human tgf β1, by R&D Systems (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Lipopolysaccharide lps, by InvivoGen (1 mentions) Sybr premix ex taq kit, by Takara Bio (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Primescript rt master mix, by Takara Bio (1 mentions) Amylase assay kit, by Nanjing Jiancheng (1 mentions) Inf39, by Selleck Chemicals (1 mentions) Ferrostatin 1 fer 1, by MedChemExpress (1 mentions) Enzyme linked immunosorbent assay elisa kits, by Solarbio (1 mentions) Rabbit anti goat antibody, by Santa Cruz Biotechnology (1 mentions) Ab157457, by Abcam (1 mentions) Ab131205, by Abcam (1 mentions) P6248, by Merck Group (1 mentions) β actin c 4 sc 47778, by Santa Cruz Biotechnology (1 mentions)

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Caerulein (107 citations)

5 protocols using caerulein cae

1

Isoliquiritigenin: Modulating Inflammatory Pathways

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ILG (Isoliquiritigenin, CAS: 961‐29‐5) was purchased from Aladdin (Aladdin Bio‐Chem Technology Company, Shanghai, China). Caerulein (Cae) and dimethyl sulfoxide (DMSO) was obtained from Sigma‐Aldrich. Recombinant human TGF‐β1 and recombinant mouse IL‐4 were obtained from R&D Systems (Minneapolis, MN, USA). Lipopolysaccharide (LPS) was purchased from InvivoGen (InvivoGen, France). Sodium carboxymethyl cellulose (Na‐CMC) was obtained from Sangon Biotechnology (Shanghai, China). Early Apoptosis Detection Kit (#6592) was obtained from Cell Signaling Technology (Danvers, Massachusetts, USA).
Wang L., He L., Hao L., Guo H., Zeng X., Bi Y., Lu G., Li Z, & Hu L. (2020). Isoliquiritigenin ameliorates caerulein‐induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophages. Journal of Cellular and Molecular Medicine, 24(17), 9667-9681.
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2

Inflammatory Responses in Pancreatitis

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Caerulein (Cae) and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (USA). Enzyme-linked immunosorbent assay (ELISA) kits for IL-1β, IL-6, and TNF-α were purchased from BioLegend. The amylase assay kit was purchased from Jiancheng Biotechnology, Nanjing. Antibodies against NLRP3, myeloperoxidase (MPO) and β-actin were purchased from Cell Signaling Technology (CST; USA). TRIzol was purchased from Invitrogen (USA). PrimeScript RT Master Mix and SYBR Premix Ex Taq™ kit were purchased from Takara (Japan). INF-39 was purchased from Selleck Chemicals (USA).
Fu Q., Zhai Z., Wang Y., Xu L., Jia P., Xia P., Liu C., Zhang X., Qin T, & Zhang H. (2018). NLRP3 Deficiency Alleviates Severe Acute Pancreatitis and Pancreatitis-Associated Lung Injury in a Mouse Model. BioMed Research International, 2018, 1294951.
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3

Assessing Ferroptosis Modulation in Cells

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P-407 and caerulein (CAE) were purchased from Sigma Chemical (St. Louis, MO, United States). Ferrostatin-1 (Fer-1) and Vas2870 (Vas) were obtained from MedChemExpress (MCE, Monmouth Junction, NJ, United States). P-407 was solubilized in phosphate buffer solution (PBS) and stored at 4 °C. CAE, Fer-1, and Vas were solubilized in dimethyl sulfoxide and stored at 20 °C. Enzyme linked immunosorbent assay (ELISA) kits were obtained from Solarbio (Beijing, China).
Meng Y.T., Zhou Y., Han P.Y, & Ren H.B. (2023). Ferroptosis inhibition attenuates inflammatory response in mice with acute hypertriglyceridemic pancreatitis. World Journal of Gastroenterology, 29(15), 2294-2309.
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4

Caerulein-Induced Mitochondrial Dynamics Analysis

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Caerulein (CAE) was purchased from Sigma-Aldrich (St. Louis, MO, United States). Rabbit monoclonal antibodies against OPA1 (ab157457, Abcam Plc, Cambridge, United Kingdom), DRP1 (D6C7 rabbit mAb; Cell Signaling), LC3B [LC3B antibody (2775)]; Cell Signaling Technology, MA, United States), p62 [SQSTM1 (P-15): sc-10117; Santa Cruz Biotechnology, Santa Cruz, CA, United States)], VMP1 (D1y3E, Cell Signaling Technology, MA, United States); mouse monoclonal antibodies against Parkin (P6248, Sigma-Aldrich, St. Louis, MO, United States), V5 [13202 V5-Tag (D3H8Q) rabbit mAB-Cell Signaling)], β-actin [β-actin (C4): sc-47778; Santa Cruz Biotechnology, Santa Cruz, CA, United States)], beta tubulin (ab131205, Abcam Plc, Cambridge, United Kingdom); rabbit anti-goat antibody (Santa Cruz Biotechnology, Santa Cruz, CA, United States); goat policlonal antibodies against VDAC-1 (D-16 sc-32063; Santa Cruz Biotechnology, Santa Cruz, CA, United States); rabbit anti-mouse antibody [(315-035-048) Jackson InmunoResearch, Baltimore Pike, United States]; goat anti-rabbit antibody [(GAR):170-5046; Bio-Rad, CA, United States]. Other reagents, enzymes, and chemicals were of reagent grade and also from Sigma-Aldrich.
Vanasco V., Ropolo A., Grasso D., Ojeda D.S., García M.N., Vico T.A., Orquera T., Quarleri J., Alvarez S, & Vaccaro M.I. (2021). Mitochondrial Dynamics and VMP1-Related Selective Mitophagy in Experimental Acute Pancreatitis. Frontiers in Cell and Developmental Biology, 9, 640094.
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5

Acute Pancreatitis Induction in Rats

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The study protocols were approved by the Animal Care and Use Committee of Guangxi Medical University (No. 201904002). All experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals.
Sixty-four 8-week-old specific-pathogen-free Sprague-Dawley healthy male rats weighing 230-250 g were randomly divided into a control group (CG, n=32) and an acute pancreatitis group (APG, n=32). All animals fasted for 12 h before treatment and had free access to water. The APG was intraperitoneally injected seven times with caerulein (CAE) (C9026, Sigma-Aldrich, USA) (50 μg/kg body weight, once an hour), and the CG was injected with saline. The rats were sacrificed and dissected 6, 12, 24, and 48 h after injection.
Yang H., Liang Z., Xie J., Wu Q., Qin Y., Zhang S, & Tang G. (2023). Gelsolin inhibits autophagy by regulating actin depolymerization in pancreatic ductal epithelial cells in acute pancreatitis. Brazilian Journal of Medical and Biological Research, 56, e12279.
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