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3 protocols using rbm25

1

Antibody Panel for Neurodegenerative Markers

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The following antibodies were purchased from Abcam: BHMT (#ab96415), CD68 (#ab955), Rbm25 (#ab72237), Synaptophysin (#ab32127). Cell signaling: β-actin (#4967), Pin1 (#3722), PSD95 (#3409). Millipore: anti-Aβ42 (#5078P), 6E10 (#MAB1560), APP C-terminal (#171610). Santa Cruz Biotechnology: H2-Ab1 (#sc-71201). Wako: Iba1 (NCNP24).
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2

Antibody-based Protein Detection Workflow

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Cell extracts and western blots were performed as previously described [55 (link)]. Antibodies used were: MED12 (Santa Cruz Biotechnology sc-515695), SOD2 (Santa Cruz Biotechnology sc-133254), LUC7L3 (ProteinTech 145041AP), EEF1B2 (ProteinTech 104831AP), BCL-Xshort (Invitrogen PA5-78864), Vinculin (Santa Cruz Biotechnology sc-73614), GAPDH (Santa Cruz Biotechnology sc-47724), Cas9 (BioLegend 844302), pChk1-S317(Cell Signaling Technology 2344S), pChk1-S345(Cell Signaling 2341S), IRF2BP2 (ProteinTech 188471AP), RPL35A (Bethyl, 501569555), MED7 (Abcam ab187146), MED13 (Invitrogen PA5-35924), CCNC (Bethyl A301-989A-T), CDK8 (ProteinTech 220671AP), TGFBR2 (Cell Signaling Technology 79424S), RNASEH2 (Bethyl A304-149A-T), RBM25 (Abcam ab72237), γH2AX (Bethyl A300-081A), phospho-SMAD2 Ser465/Ser467 (Cell Signaling Technology 18338T), MGAT4A (Abcam ab151750), MYCT1 (Abcam ab139945), BCL-Xlong, (Santa Cruz Biotechnology sc-271121), RETSAT (invitrogen PA5-65443), KNTC1 (Bethyl A301-712A-T), LIAS (Abcam ab96302).
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3

Antibody Panel for Neurodegenerative Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were purchased from Abcam: BHMT (#ab96415), CD68 (#ab955), Rbm25 (#ab72237), Synaptophysin (#ab32127). Cell signaling: β-actin (#4967), Pin1 (#3722), PSD95 (#3409). Millipore: anti-Aβ42 (#5078P), 6E10 (#MAB1560), APP C-terminal (#171610). Santa Cruz Biotechnology: H2-Ab1 (#sc-71201). Wako: Iba1 (NCNP24).
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