Ondansetron hydrochloride

“Beijing Mesochem Technology Co., Ltd. (Beijing, China)” provided apigenin (purity: 98.20 percent by HPLC). The rest of the chemicals required for this investigation were obtained from well-known suppliers, and the labels were recorded for future use. Ondansetron hydrochloride, p-chlorophenylalanine methyl ester, α-methyl-para-tyrosine methyl ester (AMPT), R(+)-SCH-23390 hydrochloride, sulpiride, phentolamine hydrochloride, and propranolol were procured from Sigma-Aldrich. Sodium chloride (0.9 percent) was used for dissolving all compounds except AMPT, whereas, the solution of AMPT was made in 10% Tween 80. Apigenin (25 and 50 mg/kg was given orally (Redrobe et al., 1998 (link)) three times before the test sessions, at 24, 5, and 1 h (Shoubaky et al., 2016 (link)). The standard antidepressant fluoxetine (30 mg/kg, oral) (Castagné et al., 2011 ) was used to compare antidepressant activities. Except for SCH23390, which was given subcutaneously, all other compounds were given by intraperitoneal route (10 ml/kg).

Drugs including 5-Hydroxytryptamine (5-HT), DNQX, 8-hydroxy-2-dipropylaminotetralin hydrobromide (8-OH-DPAT), N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251), D-AP5, 1-(m-chlorophenyl) biguanide hydrochloride (mCPBG), NAN-190, ketanserin, and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) were purchased from Tocris Bioscience (Bristol, UK). ondansetron hydrochloride, α-methyl-5-hydroxytryptamine (α-me-5-HT), CCh, and atropine were purchased from Sigma-Aldrich (St Louis, MO, USA). BAPTA was included in the pipette solution. NAN-190 and AM251 were dissolved in dimethyl sulfoxide (DMSO, 0.1%).