Cells (1.5 × 105 cells per well) were seeded in 6-well plates and treated with different concentrations of LTL (0, 10, 20, and 40 µM) for 24 h. Subsequently, cells were harvested, and apoptosis was quantitatively estimated with the Annexin V-PE/7-AAD apoptosis detection kit according to the manufacturer’s instructions. The analysis of apoptotic cells was performed using FASS-Calibur flow cytometry (BD Biosciences, San Jose, CA, USA).
Fass calibur
Manufactured by BD
Sourced in United States
The FASS-Calibur is a flow cytometry instrument designed for analyzing and sorting cells. It is capable of detecting and measuring multiple parameters of individual cells or particles in a fluid sample. The core function of the FASS-Calibur is to provide accurate and reliable cell analysis and sorting capabilities for research and clinical applications.
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Lab products found in correlation
2 protocols using fass calibur
1
Apoptosis Quantification of LTL-Treated Cells
2
Metformin-Induced Apoptosis Quantification
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Cells were plated (1.5×105 cells per well) and treated with metformin for 48 h. Subsequently, harvested and washed twice with cold PBS to remove floating cells before analysis by the Annexin V-APC Apoptosis Detection Kit (KeyGEN Biotech, Nanjing, China). Then cells were stained at room temperature with Annexin V- allophycocyanin (APC) and propidium iodide for 15 min in the dark. Apoptosis levels were quantified by a FASS-Calibur flow cytometry (BD Biosciences, San Jose, CA, USA) restricted 15 min to 1 h.
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