Anti human nuclear antigen hna

Manufactured by Merck Group

Sourced in United States

The Anti-human nuclear antigen (HNA) is a laboratory reagent used for the detection and identification of human nuclei in cell samples. It functions by binding to specific antigens present within the nuclei of human cells, allowing for their visualization and analysis.

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Lab products found in correlation

G3893, by Merck Group (1 mentions) Ab92824, by Abcam (1 mentions) M o m kit, by Vector Laboratories (1 mentions) Dxm1200f fluorescence microscope, by Nikon (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Glycerol, by Zeiss (1 mentions) Axio scope a1, by Zeiss (1 mentions)

Close spelling variants of this product

Mouse anti-human nuclear antigen (HNA; (3 citations) Human nuclear antigen (2 citations)

3 protocols using anti human nuclear antigen hna

Tracking Mitochondrial Transfer in Retina

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Mitochondrial transfer was detected via immunofluorescence on flat mounted retina. To observe mitochondrial transfer to the retina following iPSC-MSC injection, TUBB3 was used to label neuronal cytoskeleton and Brn-3a was used to stain the nucleus of RGCs. Antibody against GFP (anti-GFP; RA5-22688, Invitrogen, USA), or against human mitochondria (anti-hMito; ab92824, Abcam, USA) were used to label human mitochondria originated from iPSC-MSCs. Anti-human nuclear antigen (HNA; Millipore, USA) was used to identify human cells. Retinal samples were examined using a laser scanning confocal microscope LSM700 or a time-lapse video recorder. To identify the precise location of the mitochondria in the mouse retina, z-stack scanning strategy, and orthogonal section of the retina were carried out for further image analysis. Evaluation of the average immunoreactivity of GFP signal from the retinal ganglion cell layer (GCL) was compared among different time points of post iPSC-MSC treatment.
To evaluate Müller cell activation following iPSC-MSC therapy, immunohistochemistry of glial fibrillary acidic protein (GFAP; G3893, Sigma, USA) on the retinal sections was performed.

Jiang D., Xiong G., Feng H., Zhang Z., Chen P., Yan B., Chen L., Gandhervin K., Ma C., Li C., Han S., Zhang Y., Liao C., Lee T.L., Tse H.F., Fu Q.L., Chiu K, & Lian Q. (2019). Donation of mitochondria by iPSC-derived mesenchymal stem cells protects retinal ganglion cells against mitochondrial complex I defect-induced degeneration. Theranostics, 9(8), 2395-2410.

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Immunofluorescent Staining of Tissue Sections

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Indirect immunofluorescence staining was performed using a standard procedure. In brief, tissues cryosectioned at a 4-μm thickness were fixed with 4% paraformaldehyde, blocked with 5% BSA/PBS (1 h, 24℃), washed twice with PBS, treated with 0.1% Triton X-100/PBS for 1 min, and washed extensively in PBS. The sections were stained with specific primary antibodies and fluorescent-conjugated secondary antibodies (Supplementary Table S2) using a M.O.M kit according to the manufacturer’s instructions (Vector Laboratories, Burlingame, CA). The cells were counterstained with DAPI (4,6-diamino-2-phenylindole dihydrochloride; Vector Laboratories). Mouse IgG (Dako, Carpinteria, CA) and rabbit IgG (Dako) antibodies was used as negative controls. To detect transplanted human cells, sections were immunofluorescently stained with anti-human nuclear antigen (HNA, Millipore). The stained sections were viewed with a DXM1200F fluorescence microscope (Nikon, Tokyo, Japan). The processed images were analyzed for fluorescence intensity using the ImageJ software (NIH).

, & Park I.S. (2021). Enhancement of Wound Healing by Conditioned Medium of Adipose-Derived Stromal Cell with Photobiomodulation in Skin Wound. International Journal of Stem Cells, 14(2), 212-220.

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Tracking Stem Cell Migration in Lymph Nodes

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The post-fixed cervical lymph nodes were cryoprotected in 30% sucrose, and frozen 10 μm thickness sections were prepared. The reporter green fluorescent protein gene expression in the cervical lymph nodes in rats from the Ad5-GFP and UCBC + Ad5-GFP groups was evaluated using fluorescence microscopy. For identification of human UCBC in lymph nodes in rats from the UCBC + Ad5-GFP and UCBC + Ad5-LTF groups, immunofluorescent staining was performed using mouse antibody (Ab) reacting with anti-human nuclear antigen (HNA) (1:150, Millipore, Burlington, MA, USA). Secondary donkey Alexa Fluor 647 conjugated Ab (1:200, Invitrogen) was used for visualization of the primary Ab. Cell nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole − DAPI (10 μg/mL in PBS, Sigma). Processed sections were embedded in glycerol (GalenoPharm; Saint Petersburg, Russia) and examined with a luminescence microscope (Carl Zeiss Axioscope A1). UCBC (HNA-positive cells) were counted in UCBC + Ad5-GFP and UCBC + Ad5-LTF groups in 10 sections of the cervical lymph node from each rat. Cell counting was performed on digital images in an area of 300 × 240 µm. Average cell counts for each animal were standardized to the area of 1 mm².

Agatieva E., Ksembaev S., Sokolov M., Markosyan V., Gazizov I., Tsyplakov D., Shmarov M., Tutykhina I., Naroditsky B., Logunov D., Pozdeev O., Morozova L., Yapparova K, & Islamov R. (2021). Evaluation of Direct and Cell-Mediated Lactoferrin Gene Therapy for the Maxillofacial Area Abscesses in Rats. Pharmaceutics, 13(1), 58.

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