All phenolic acid standards, Trolox, 2,2-diphenyl−1-picrylhydrazyl radical (DPPH•), 2,2′-azino-bis-3(ethylbenzthiazoline-6-sulphonic acid) (ABTS•+), soybean 15-lipooxygenase, xanthine oxidase, linoleic acid, allopurinol, quercetin, α-glucosidase, p-nitrophenyl-α-D-glucopyranoside, α-amylase, starch solution, dinitrosalicylic acid, Rochelle salt, and acarbose were provided by Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Methanol, ethanol, chloroform, diethyl ether, sodium bicarbonate, hydrogen chloride, anhydrous sodium sulphate, sodium borohydride, sulphuric acid, sodium carbonate, sodium hydroxide, and phosphate buffer were purchased from Avantor Performance Materials Poland (Gliwice, Poland). All the chemicals were of analytical grade. LC grade Methanol and formic acid were purchased from J.T. Baker (Phillipsburg, NJ, USA). LC grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA).
Direct q3 purification system
Manufactured by Merck Group
Sourced in United States, France
The Direct-Q3 purification system is a water purification device that produces ultrapure water for laboratory and research applications. It utilizes a combination of filtration and ion exchange technologies to remove contaminants and produce water with high purity levels.
Automatically generated - may contain errors
Lab products found in correlation
Vanillic, by Merck Group (4 mentions)
Ferulic, by Merck Group (4 mentions)
Gallic, by Merck Group (4 mentions)
P coumaric, by Merck Group (4 mentions)
Protocatechuic, by Merck Group (4 mentions)
Salicylic, by Merck Group (4 mentions)
Taxifolin, by Merck Group (3 mentions)
Isorhamnetin, by Merck Group (3 mentions)
Caffeic, by Merck Group (3 mentions)
Gentisic, by ChromaDex (3 mentions)
3 o methylquercetin, by Merck Group (3 mentions)
Luteolin, by Merck Group (3 mentions)
Kaempferol, by Merck Group (3 mentions)
Syringic, by Merck Group (3 mentions)
Sakuranetin, by Merck Group (3 mentions)
Apigenin, by Carl Roth (3 mentions)
Myricetin, by ChromaDex (3 mentions)
Isoferulic, by Merck Group (3 mentions)
Eriodictyol, by ChromaDex (3 mentions)
Naringenin, by Carl Roth (3 mentions)
23 protocols using direct q3 purification system
1
Antioxidant and Enzyme Inhibition Assays
2
Phenolic Compounds Quantification Protocol
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Ethanol and sodium carbonate were purchased from Avantor Performance Materials Poland (Gliwice, Poland). Gallic, m-coumaric, o-coumaric, p-coumaric, caffeic, 5-O-caffeoylqunic, ferulic, isoferulic, vanillic, salicylic, 3-hydroxybenzoic, 4-hydroxybenzoic, protocatechuic, syringic, rosmarinic acid, quercitrin, apigenin 7-O-glucoside, eriocitrin, taxifolin, 3-O-methylquercetin, isorhamnetin, isorhamnetin-3-O-glucoside, luteolin, kaempferol, rutin, hyperoside, isoquercetin, sakuranetin, aluminum chloride, and LC–MS grade acetonitrile were purchased from Sigma-Aldrich Chemical Co. (St. Louis, Mo, USA). Folin–Ciocalteu reagent was supplied by Chempur (Piekary Śląskie, Poland). Quercetin was purchased from Fluka (Buchs, Switzerland). Catechin, naringenin 7-O-glucoside, eriodictyol, luteolin-7-O-glucoside, naringenin 7-O-glucoside, luteolin 3,7-diglucoside, nicotiflorin, narcissoside, gentisic, sinapic acid, and myricetin were provided by ChromaDex (Irvine, CA, USA). Astragalin, apigenin, naringenin, kaempferol-3-rutinoside, and tiliroside were supplied by Roth (Karlsruhe, Germany). LC–MS grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA).
3
Quantifying Polyphenol Standards Analysis
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Standards of caffeic, gallic, ferulic, isoferulic, protocatechuic, 4-OH-benzoic, vanillic, p-coumaric, salicylic, and LC grade acetonitrile were purchased from Sigma–Aldrich Fine Chemicals (St. Louis, MO, USA). Gentisic and sinapic acids were from ChromaDex (Irvine, CA, USA). The LC grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). Ethanol and methanol (reagent grade purity), used for the preparation of extracts, Folin-Ciocalteu reagents, and all analytical grade reagents were obtained from Avantor Performance Materials (Center Valley, PA, USA).
4
Antioxidant and Enzyme Inhibition Assays
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1,1-Diphenyl-2-picryl-hydrazyl (DPPH), linoleic acid, EDTA, BHA, ascorbic acid, α-tocopherol, DMSO, physostigmine, hyaluronidase from bovine testes type I-S, Streptococcus equi hyaluronic acid, DTNB (5,5′-Dithiobis(2-nitrobenzoic acid), ACTI (acetylthiocholine iodide), sodium phosphate buffer pH 7.0 were obtained from Sigma-Aldrich. The standards of phenolic acids were obtained from ChromaDex (Santa Ana, CA). LC grade methanol (MeOH) was purchased from J.T. Baker (Phillipsburg, USA). Ultrapure water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). All others reagents were of analytical grade.
5
Analytical Standards for Phytochemicals
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Analytical standards of apigetrin (apigenin 7-glucoside), chrysin, prunetin, rhamnazin, morin, isoquercetin, vitexin, isovitexin, quercitrin, kaempferitrin, taxifolin, luteolin, 3-O-methylquercetin, kaempferol, hyperoside, rutin, isorhamnetin, isokaempferide, rhamnetin, sakuranetin, luteoloside, eriodictyol-7-O-glucopyranoside, narirutin, naringin, LC grade acetonitryl, DMSO, DPPH radical, ampicillin, bovine serum, ferric chloride, hyaluronidase from bovine testes type I -S, sodium phosphate buffer pH 7, oleanolic acid, 2,4,6-tris(2-pyridyl)-s-triazine TPTZ, and hyaluronic acid were obtained from Sigma-Aldrich Fine Chemicals (St. Louis, MI, USA). Naringenin, astragalin, apigenin, tiliroside, and nicotiflorin were purchased from Roth (Karlsruhe, Germany). Dihydromyricetin was supplied by LGC (LGC Group, Teddington, UK), and laricitrin by Extrasynthese (Lyon, France). Quercetin was obtained from Fluka (Buchs, Switzerland). Catechin, luteolin-7-O-glucoside, luteolin 3,7-diglucoside, Naringenin 7-O-glucoside, eriodictyol, and myricetin were supplied by ChromaDex (Irvine, CA, USA). LC-MS grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). Ethanol was obtained from Avantor Performance Materials (Gliwice, Poland).
6
Quantification of Phytochemicals in Eleutherococcus
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Ethanol was obtained from POCH (Polish Chemicals Reagents, Lublin, Poland). The standards of eleutheroside B ≥ 98.0% (HPLC), eleutheroside E ≥ 98.0% (HPLC) and naringenin ≥98.0% (HPLC), were obtained from Sigma-Adrich. LC-grade methanol (Methanol gradient grade for Liquid Chromatography) was purchased from J.T. Baker (Phillipsburg, NJ, USA). Ultrapure water was prepared using the Millipore Direct-Q3 purification system (Bedford, MA, USA). All other reagents were of analytical grade.
7
Graphene oxide preparation and boron adsorption
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The aqueous solutions used in this work were prepared using deionized water (18.2 MΩ cm at 25 °C) obtained from a Direct-Q 3 purification system from Millipore (Bedford, MA, USA).
The preparation and functionalization of GO were carried out using the following reagents: graphite (Aldrich Chemistry, mesh 325, 99%), sodium nitrate (Vetec, Rio de Janeiro, Brazil), concentrated sulfuric acid (Tedia, Fairfield, OH, USA), potassium permanganate (Vetec, Rio de Janeiro, Brazil), 30% m/v hydrogen peroxide solution (Sigma-Aldrich, St. Louis, USA), concentrated hydrochloric acid (37% w/w) (J. T. Baker, Mexico City, Mexico), N-methyl-d -glucamine (Sigma-Aldrich, St. Louis, MO, USA), dimethylformamide (J. T. Baker, Mexico City, Mexico), absolute ethanol (J. T. Baker, Mexico City, Mexico).
The standard boron solutions were prepared by appropriate dilution of an individual stock solution of boron with a concentration of 1000 mg L−1 (Sigma-Aldrich, St. Louis, USA) in a 1% v/v HNO3 solution. All standard solutions were prepared immediately before use, and the addition of HNO3 was made to ensure the stability of the solutions. The concentrated HNO3 (65% w/w) used in this work was supplied by Merck (Darmstadt, Germany) and was also used in the microwave-assisted digestion of the boron-loaded adsorbent obtained after the adsorption procedure.
The preparation and functionalization of GO were carried out using the following reagents: graphite (Aldrich Chemistry, mesh 325, 99%), sodium nitrate (Vetec, Rio de Janeiro, Brazil), concentrated sulfuric acid (Tedia, Fairfield, OH, USA), potassium permanganate (Vetec, Rio de Janeiro, Brazil), 30% m/v hydrogen peroxide solution (Sigma-Aldrich, St. Louis, USA), concentrated hydrochloric acid (37% w/w) (J. T. Baker, Mexico City, Mexico), N-methyl-
The standard boron solutions were prepared by appropriate dilution of an individual stock solution of boron with a concentration of 1000 mg L−1 (Sigma-Aldrich, St. Louis, USA) in a 1% v/v HNO3 solution. All standard solutions were prepared immediately before use, and the addition of HNO3 was made to ensure the stability of the solutions. The concentrated HNO3 (65% w/w) used in this work was supplied by Merck (Darmstadt, Germany) and was also used in the microwave-assisted digestion of the boron-loaded adsorbent obtained after the adsorption procedure.
8
Cytotoxicity Assay with Podophyllotoxin
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Podophyllotoxin (standard compound, ≥98%), MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], LC grade acetonitrile, and RPMI 1640 medium were purchased from Sigma-Aldrich Fine Chemicals (Saint Louis, MO, USA), Fetal calf serum (FCS) for cell culture was purchased from Biochrom GmbH (Berlin, Germany), and DMSO was purchased from Fluka Chemie AG (Buchs, Switzerland). LC grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA).
9
Analytical Reagent Grade Solvents
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All chemicals were of analytical reagent grade. Acetonitrile and formic acid were purchased from Biosolve Chemicals.
Deionized water was obtained from a Direct-Q3 purification system (Millipore).
Deionized water was obtained from a Direct-Q3 purification system (Millipore).
10
Phenolic Acids Analysis in Kale
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Standards of phenolic acids were purchased from Sigma-Aldrich Fine Chemicals (St. Louis, MO, USA). All the chemicals were of analytical grade. LC grade methanol (MeOH) was purchased from J.T. Baker (Phillipsburg, USA). LC grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). Brassica oleracea L. var. sabellica (kale) was purchased from “Klasa” company (Kurów, Poland). The plant was dried in the convection dryer at an average temperature of 39.0°C. The dry plant material was milled to particle size lower than 500 μm and sieved.
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