Millipore direct q 5uv system

N-(2-fluorphenyl)-6-chloro-4-hydroxy-2-quinolone-3-carboxamide (R19) was synthesized and characterized as previously described [9 (link)]. Pluronic P123 (P123), TPGS, and Nile red (NR) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered saline solution (PBS, 10×, pH 7.4), potassium bromide (KBr), and dimethyl sulfoxide (DMSO) were procured from Fisher Chemicals (Pittsburgh, PA, USA). Spectra/Por dialysis membranes with 3500 and 12,000–14,000 Da molecular weight cut-offs (MWCO) were obtained from Repligen (Waltham, MA, USA). Ultrapure water was prepared using a Millipore Direct-Q 5UV system (EMD Millipore, Burlington, MA, USA).

SBECD solutions with increasing concentrations were prepared using ultrapure water (Millipore Direct-Q 5UV system, Merck Millipore, Burlington, MA, USA), and the same amounts of OTX008 were tested to dissolve in them to reveal the solubilization ability of SBECD on OTX008. OTX008 was clearly dissolved in the 7.3 m/m% SBECD solution at a 0.75 mg/mL final concentration to obtain the OTX008-SBECD solution.

Tin(II) 2-ethylhexanoate (stannous octoate), ε-caprolactone (CL), methoxy poly(ethylene glycol) (mPEG) molecular weight (MW) 5000, Span 80, and thymoquinone (TQ) were obtained from Sigma Aldrich (St. Louis, MO, USA). Castor oil was provided by Philadelphia Pharmaceuticals (Amman, Jordan). Polysorbate 80 (Tween 80,) was purchased from RFCL Ltd. (New Delhi, India). Absolute ethanol, acetone, methanol (HPLC grade), isopropanol, formic acid, water (HPLC grade), and dichloromethane (DCM) were obtained from Fisher Chemical (Thermo Fisher Scientific, Waltham, MA, USA). Diethyl ether and dimethyl sulfoxide were provided by Tedia (Fairfield, OH, USA). Phosphate buffered saline (PBS) 10×, pH 7.4, was obtained from Biowest (Nuaillé, France). Ultrapure water (~18.2 MΩ·cm) was prepared using a Millipore Direct-Q 5UV system (EMD Millipore, Billerica, MA, USA).