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Influenza hemagglutinin h1cal2009

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Influenza hemagglutinin (H1Cal2009) is a recombinant protein produced by Sino Biological. Hemagglutinin is a surface glycoprotein found on the influenza virus that plays a key role in the virus's ability to bind to and infect host cells.

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Lab products found in correlation

Magnetic carboxylated beads, by Bio-Rad (3 mentions) Tetanus toxoid, by Merck Group (3 mentions) Siv gp120, by Sino Biological (3 mentions) Spike 2, by ACROBiosystems (2 mentions) Sars cov 2 spike 1, by Sino Biological (2 mentions) Sars cov 2 and hku 1 spike trimers, by BPS Biosciences (2 mentions) Sars cov and nl63 spike trimers, by BPS Biosciences (2 mentions) Nucleoprotein, by ACROBiosystems (1 mentions) Sars cov 2 spike trimers, by BPS Biosciences (1 mentions)

3 protocols using influenza hemagglutinin h1cal2009

1

Multiplex SARS-CoV-2 Antibody Detection

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As previously described25 , a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Fig. 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were also included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B., Selva K., Kedzierski L., Ashhurst T., Haycroft E., Shoffner S., Hensen L., Boyd D., James F., Mouhtouris E., Kwong J., Chua K., Drewett G., Copaescu A., Dobson J., Rowntree L., Habel J., Allen L., Koay H.F., Neil J., Gartner M., Lee C., Andersson P., Seemann T., Sherry N., Amanat F., Krammer F., Londrigan S., Wakim L., King N., Godfrey D., Mackay L., Thomas P., Nicholson S., Arnold K., Chung A., Holmes N., Smibert O., Trubiano J., Gordon C., Nguyen T, & Kedzierska K. (2021). Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity. Research Square.
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2

SARS-CoV-2 Multiplex Serological Assay

Cited 1 time
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As previously described25 (link), a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Table 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B.Y., Selva K.J., Kedzierski L., Ashhurst T.M., Haycroft E.R., Shoffner-Beck S.K., Hensen L., Boyd D.F., James F., Mouhtouris E., Kwong J.C., Chua K.Y., Drewett G., Copaescu A., Dobson J.E., Rowntree L.C., Habel J.R., Allen L.F., Koay H.F., Neil J.A., Gartner M.J., Lee C.Y., Andersson P., Khan S.F., Blakeway L., Wisniewski J., McMahon J.H., Vine E.E., Cunningham A.L., Audsley J., Thevarajan I., Seemann T., Sherry N.L., Amanat F., Krammer F., Londrigan S.L., Wakim L.M., King N.J., Godfrey D.I., Mackay L.K., Thomas P.G., Nicholson S., Arnold K.B., Chung A.W., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Nguyen T.H, & Kedzierska K. (2022). SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity. Nature Communications, 13, 2774.
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3

Multiplex Bead Array for COVID-19 Serology

Cited 1 time
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A custom multiplex bead array was designed (Supplemental Table 3) and coupled with SARS-CoV-1 and SARS-CoV-2 spike-1 (stem, Sino Biological), spike-2 (head, ACRO Biosystems), RBD (BEI Resources), and N (ACRO Biosystems), as described previously (45 (link)). In addition, SARS-CoV-2 spike trimers (provided in-house) and SARS-CoV-2 spike trimers (BPS Bioscience) were included. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009, Sino Biological), and SIV-gp120 (Sino Biological) were included as positive and negative control antigens, respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio-Rad) using a 2-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS with 0.05% sodium azide until use.
Habel J.R., Chua B.Y., Kedzierski L., Selva K.J., Damelang T., Haycroft E.R., Nguyen T.H., Koay H.F., Nicholson S., McQuilten H.A., Jia X., Allen L.F., Hensen L., Zhang W., van de Sandt C.E., Neil J.A., Pragastis K., Lau J.S., Jumarang J., Allen E.K., Amanant F., Krammer F., Wragg K.M., Juno J.A., Wheatley A.K., Tan H.X., Pell G., Walker S., Audsley J., Reynaldi A., Thevarajan I., Denholm J.T., Subbarao K., Davenport M.P., Hogarth P.M., Godfrey D.I., Cheng A.C., Tong S.Y., Bond K., Williamson D.A., McMahon J.H., Thomas P.G., Pannaraj P.S., James F., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Chung A.W., Whitehead C.L., Kent S.J., Lappas M., Rowntree L.C, & Kedzierska K. (2023). Immune profiling of SARS-CoV-2 infection during pregnancy reveals NK cell and γδ T cell perturbations. JCI Insight, 8(7), e167157.
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