Cyclic voltammetry (CV) and Chronoamperometric (CA) measurements were performed by using CHI 660E electrochemical workstation (CH Instruments Inc., Austin, TX, USA). A three electrode cell had platinum wire (ø 0.5 mm, CHI115, from CH Instruments Inc.) as a counter electrode and a saturated calomel reference electrode (SCE, CHI150, from CH Instruments Inc.) was used for electrochemical measurements. During the CV measurement, initial potential is post as 0.0 V, and the initial scan direction is negative. All experiments were conducted at room temperature (about 26 °C).
Chi115
Manufactured by CH Instruments
Sourced in United States
The CHI115 is a potentiostat/galvanostat that can be used for electrochemical measurements. It provides a voltage range of ±10 V and a current range of ±250 mA. The device is capable of performing various electrochemical techniques such as cyclic voltammetry, chronoamperometry, and chronopotentiometry.
Automatically generated - may contain errors
Lab products found in correlation
Chi111, by CH Instruments (5 mentions)
Chi150, by CH Instruments (1 mentions)
Chi 660e, by CH Instruments (1 mentions)
Rhd2000 interface software, by Intan Technologies (1 mentions)
Mf 2052, by Bioanalytical Systems (1 mentions)
Chi1140a, by CH Instruments (1 mentions)
Mercaptohexanol, by Merck Group (1 mentions)
Vancomycin, by Integrated DNA Technologies (1 mentions)
Vancomycin, by Merck Group (1 mentions)
Chi101, by CH Instruments (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride, by Merck Group (1 mentions)
Hydrogen peroxide, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Cystamine dihydrochloride, by Merck Group (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
A412 4, by Thermo Fisher Scientific (1 mentions)
Chi104, by CH Instruments (1 mentions)
8 protocols using chi115
1
Electrochemical Characterization of Materials
2
Electrochemical Synthesis of Molecularly Imprinted Polymer Sensor
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The rGO/GCE was employed in an electrochemical cell as a WE along
with a counter electrode (CE, CHI115, CH Instruments Inc., Austin,
USA) and reference electrode (RE, CHI111, CH Instruments Inc., USA).
For the electropolymerization of MIP, the electrolyte was 1.0 ×
10–3 mol L–1 ANI, 1.0 × 10–3 mol L–1o-PDA,
and 5.0 × 10–3 mol L–1 CIP
as a template in a background medium of HCl solution (pH 3). The electropolymerization
was performed in a cyclic voltammogram. A potential window of 0.0
to 0.7 V was applied to WE at 80 mV s–1 scan rate
for 15 cycles (Figure S4a ). The MIP-modified
electrode was eluted by immersion in 1.0 mol L–1 HCl for 3 min, creating imprinted cavities on the MIP. MIP/rGO/GCE
was thoroughly rinsed with UPW and stored in a desiccator for future
use. In addition, NIP/rGO/GCE was fabricated following a similar procedure
with no CIP during the electropolymerization. The CV curve obtained
from the electropolymerization is illustrated inFigure S4b .
with a counter electrode (CE, CHI115, CH Instruments Inc., Austin,
USA) and reference electrode (RE, CHI111, CH Instruments Inc., USA).
For the electropolymerization of MIP, the electrolyte was 1.0 ×
10–3 mol L–1 ANI, 1.0 × 10–3 mol L–1o-PDA,
and 5.0 × 10–3 mol L–1 CIP
as a template in a background medium of HCl solution (pH 3). The electropolymerization
was performed in a cyclic voltammogram. A potential window of 0.0
to 0.7 V was applied to WE at 80 mV s–1 scan rate
for 15 cycles (
electrode was eluted by immersion in 1.0 mol L–1 HCl for 3 min, creating imprinted cavities on the MIP. MIP/rGO/GCE
was thoroughly rinsed with UPW and stored in a desiccator for future
use. In addition, NIP/rGO/GCE was fabricated following a similar procedure
with no CIP during the electropolymerization. The CV curve obtained
from the electropolymerization is illustrated in
3
In Vivo Electrochemical Impedance Spectroscopy of DRG Electrodes
4
Electrochemical impedance of carbon fiber electrodes
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The electrochemical impedances of carbon fiber electrodes before and after fire-sharpening process was measured at 1 kHz in phosphate-buffered saline (PBS) solution using a 3-electrode based potentiostat system (ZIVE SP2; ZIVE lab., Seoul, Korea) with Ag/AgCl electrode (MF-2052; Bioanalytical Systems, Inc., West Lafayette, IN, USA) as a reference electrode and Pt electrode (CHI115; CH Instruments, Inc., Austin, TX, USA) as a counter electrode. After electrode implantation into the rat brain, the impedances of the carbon fiber electrode embedded with silk and tungsten supports were measured every two days for 1 month. Since there was no counter electrode, the impedance was measured using 2-electrode configuration with the implanted silver wire as a reference electrode and the carbon fiber as a working electrode using RHD2000 interface software (Intan technologies, Los Angeles, CA, USA). The impedance magnitudes measured at 1 kHz were compared between tungsten- and silk-support electrode arrays.
5
Electrochemical Study of Gold-Protein Interactions
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The interactions of gold compounds with protein have been performed in solution by electrochemical investigations Voltammetry measurements were performed with an electrochemical workstation (CHI1140A, CH Instruments Inc., Austin, TX, USA). The Ag/AgCl reference electrode (in 3M KCl, CHI111, CH Instruments Inc), Glassy carbon working electrode (CHI 112, CH Instruments Inc) and platinum wire counter electrode (CHI115, CH Instruments Inc) inserted into the 5.0 ml glass cell. Due to the poor solubility of the present compounds in water, their solutions were prepared in ethanol. The GCE was polished as a mirror like surface with alumina slurry on the synthetic cloth before every electrochemical analysis. The cyclic voltammetry (CV) and square wave voltammetry (SWV) were scanned from −0.4 to 1.0 V for various analyses for compounds 1–3 in the absence and in the presence of different concentration of lysozyme under physiological environment.
6
Quantitative Electrochemical Aptamer Testing
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Mercaptohexanol (MCH), phosphate buffered saline (PBS), MgCl, Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), serum, and vancomycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). vancomycin aptamer, [/5ThioMC6-D/CGAGG GTACC GCAAT AGTAC TTATT GTTCG CCTAT TGTGG GTCGG/3MeBlN/] with conjugated methylene blue and thiol ends, was purchased from Integrated DNA Technologies (Coralville, IA, USA) and has been previously characterized. [18 (link)] The vancomycin aptamer is often used as a vehicle for wider aptamer research and was therefore an ideal test aptamer for proof-of-concept testing with peak-tracking software (vancomycin detection is not the focus of this paper). Controlled-temperature test enclosures (Happybuy Reptile Incubator 25L) and faradaic shields (TACKMETER WiFi Router Shield, and Electriduct ½” Tinned Copper Metal Braid) were purchased from Amazon (Seattle, CA, USA). A 2 mm gold rod (CHI101), Ag/AgCl reference (CHI111), and Pt counter electrodes (CHI115) were all purchased from CH Instruments (Austin, TX, USA). Polishing pads, and 0.3- and 0.05-micron alumina slurry (ET030) were purchased from eDAQ (Colorado Springs, CO, USA).
7
Electrochemical Characterization of 2'FL
8
Electrochemical Analysis of Omega-3 Oils
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Commercial omega-3 fish oil purified from sardines and anchovies and supplemented with tocopherol was obtained from Nordic Naturals (YE-1022/1680677, Watsonville, CA). Aluminum oxide (199974-1 KG, Sigma-Aldrich, St. Louis, MO), sand (S25516B, Fisher Science, Waltham, MA), Pyrex glass wool (3950, Corning Inc., Corning, NY), and hexane at 95% purity (H306-4, Fisher Science) were used for stripping tocopherols from the commercial oil. Tocopherol standards, α-(T3251, Sigma-Aldrich), δ-(47784 Supelco, Bellefonte, PA) and γ (T028, Sigma-Aldrich) were purchased for electrochemical experiments. The solvent used for electrochemical testing consisted of sulfuric acid (A300-500, Fisher Scientific), benzene (BX0220-5, EMD Millipore, Darmstadt, Germany) and methanol (A412-4, Fisher Chemical). DPV analysis was performed using a CH Instruments model 660E SN:A3192 using a 3-electrode configuration consisting of a platinum counter electrode (CHI115, CH Instruments, Austin, TX), silversilver/chloride reference electrode (CHI111, CH Instruments) and a glass carbon working electrode (CHI104, CH Instruments).
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