Affymetrix human genome u219 array strip

DMU-214 was synthesized as described elsewhere [21 (link)]. The identity and purity of each compound were confirmed by NMR and GC-mass spectroscopy. The QCM Chemotaxis Migration Assay was purchased from Merck (Darmstadt, Germany). The Affymetrix^® Human Genome U219 Array Strip was provided by Affymetrix (Santa Clara, CA, USA).

BNF, SYBR^® Green I (10,000× concentration), agarose, JumpStart Taq DNA polymerase, Enhanced Avian RT first-strand synthesis kit (STR-1), GenElute™ PCR Clean-Up Kit, GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, PCR Low Ladder Marker Set, guanidine thiocyanate, ammonium thiocyanate, Williams’ E medium, LB broth, and LB agar were supplied by Sigma-Aldrich Co (St. Louis, MO, USA). Fluorescein was obtained from Bio-Rad Laboratories (Hercules, CA, USA). Restriction endonucleases were purchased from Fermentas International Inc. (Burlington, Canada). Deoxyribonucleotide triphosphates such as dATP, dGTP, aCTP, and dTTP were provided by Roche Diagnostics (Mannheim, Germany). PCR primers were provided by Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Poland (oligo.pl), and Genomed, Poland. Agilent RNA 6000 Reagents were provided by Agilent Technologies (Santa Clara, CA, USA). Affymetrix Human Genome U219 Array Strip, GeneChip 3′IVT Express Kit and GeneAtlas Hybridization, and Wash and Stain Kit for 3′IVT Arrays were provided by Affymetrix (Santa Clara, CA, USA). GeneClip™ U1 Hairpin Cloning System—Neomycin Vector and antibiotic G418 (Geneticin)—was provided by Promega (Madison, WI, USA). Lipofectamine 2000 and Opti-MEM^® I Reduced Serum Medium was provided by Invitrogen (Carlsbad, CA, USA). All the other compounds were readily available as commercial products.

The metabolites of DMU-212 were synthesized as described elsewhere6 (link). The identity and purity of each compound was confirmed by NMR and GC-mass spectroscopy. Propidium iodide (PI), ribonuclease A (RNase A), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were provided by Sigma-Aldrich Co. (St. Louis, MO, USA). The Cell Death Detection ELISA^PLUS kit was acquired from Roche Diagnostics (Germany). Caspase-Glo^® 3/7, Caspase-Glo^® 8 and Caspase-Glo^® 9 Assay Kits were purchased from Promega Co. (USA). The Affymetrix® Human Genome U219 Array Strip was provided by Affymetrix (Santa Clara, CA, USA). The Proteome Profiler Human Apoptosis Array kit was obtained from R&D Systems (Minneapolis, Minnesota, USA). Forane (isoflurane) was acquired from Abbott Laboratories Ltd. (Illinois, USA). D-Luciferin (potassium salt) was obtained from Gold Biotechnology (St. Louis, MO, USA).