1 bis dimethylamino methylene 1h 1 2 3 triazolo 4 5 b pyridinium 3 oxide hexafluorophosphate hatu

Cis-Dichlorodiamine platinum (II) (cisplatin) was purchased from Strem Chemicals, Inc. (Newburyport, MA). Sodium hyaluronate was purchased from Contipro Inc (Dolní Dobrouč, Czech Republic). 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU), and 3-(3-Dimethylaminopropyl)-1-ethyl-carbodiimide hydrochloride (EDC-HCl) was purchased from Chem-Impex International, Inc. (Wood Dale, IL). 1-Hydroxybenzotriazole hydrate (HOBt•H₂O) was purchased from Advanced ChemTech (Louisville, KY). N-(tert-Butoxycarbonyl)glycine (Boc-Gly-OH), hydrogen peroxide solution (H₂O₂, 30 wt.% in H₂O), succinic anhydride, (±)-α-tocopherol, N,N′-Dicyclohexylcarbodiimide (DCC), N,N-Dimethylpyridin-4-amine (DMAP), trifluoroacetic acid (TFA), N,N-Diisopropylethylamine (DIPEA), oxaliplatin, tetrabutylammonium hydroxide solution (TBA-OH, 40 wt.% in H₂O), and anhydrous organic solvents were purchased from Sigma-Aldrich (Saint Louis, MO). Alexa Fluor ® 488 anti-CD8a, Alexa Fluor® 594 anti-CD11b, and Alexa Fluor® 647 anti-CD11c antibodies were purchased from BioLegend (San Diego, CA). All other chemicals and organic solvents were purchased from Thermo Fisher Scientific (Waltham, MA).

N-Fluorenylmethyloxycarbonyl (Fmoc) protected amino acids, amino acid attached 2-chlorotrityl resins, 1-[bis(dimethylamino) methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluoroph osphate (HATU), and 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Chem-Impex International, Inc. (Wood Dale, IL) and AAPPTEC LLC (Louisville, KY). Potassium hexacyanoferrate (II) trihydrate, potassium nitrate, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysulfosuccinimide (Sulfo-NHS), 6-mercapto-1-hexanol, and 6-mercapto-1-hexanoic acid were obtained from Sigma-Aldrich (St. Louis, MO). Dithiothreitol (DTT), sodium phosphate dibasic heptahydrate (Na₂HPO₄•7H₂O), sodium phosphate monobasic (NaH₂PO₄) and 2-(4-morpholino)ethanesulfonic acid (MES) were purchased from Fisher Scientific (Hampton, NH). Purified recombinant human cathepsin B (~60% 37 kDa inactive form and ~40% 25 kDa active form) was acquired from R&D Systems Inc. (Minneapolis, MN). Cathepsin B solutions were activated by incubation in 25 mM MES buffer (pH = 5.0) containing 5 mM DTT for 15 min before proteolysis experiments.