Acl25416u a

On-field experiments were realized thanks to carry-on detection equipment. It consists in two blue LEDs (Thorlabs – M490L3–490 nm) combined with a first lense (Thorlabs ACL25416U-A), two filters (Thorlabs FES0500 – Thorlabs M497-16) and a second lense (Thorlabs LA1422-A) to light the paper. The signal emitted by the biological reaction on paper is collected by a linear camera (Thorlabs LC-100) through a first lense (Thorlabs ACL3026-A), a FITC dichroic filter (Thorlabs MD499), a filter (Thorlabs FELH0500) and a second lense (Thorlabs ACL25416U-A). The paper device is positioned on a chip holder heated by a PTC heater (DBK HP05), controlled by a platine thermic element (RS Components PT 1000ohms) and a temperature controller (Carel IR33). A home-made Microsoft Office 2007 macro program enables to extract data from the linear camera recording software (Splicco), to detect the position of the maximum fluorescence intensity for each paper rectangular area and to monitor the mean intensity around each maximum over time.

Six identical custom single-color epi-fluorescence microscopes were constructed using Thorlabs optomechanics, lenses and stages in a 30 mm cage system mounted to an optical table, with an MA845 20x, 0.65 NA objective (Meiji Techno) mounted in an SM1Z linear stage (Thorlabs) for focusing. The field of view was approximately 240 μm × 160 μm. Excitation illumination was provided by a high-power lime M565D2 LED (Thorlabs) controlled by a relay module and filtered using 49004 dTomato-appropriate filter-dichroic sets (Chroma). Illumination from the LED was collimated using a 0.79 NA ACL25416U-A aspheric condenser lens, and images were produced from the infinity corrected objective using an AC254-200-A-ML achromatic doublet (Thorlabs). Images were captured by mono-color CM3-U3-13S2M-CS CCD cameras (Point Grey) controlled over USB 3.0.

Our setup uses three high-power color LED chips (LXZ1-PB01, LHUV-0400, and LXZ1-PX01; Lumileds, NL) as excitation lights for the green (such as the RSFPs that are used in this work) and red (such as DsRed, which is subsequently used in an application development) fluorescent emitters. The sources are collimated by high-NA condensers (ACL25416U-A, f = 16 mm, Thorlabs, NJ, USA) and filtered by bandpass filters (ET470/40×, ET402/15×, ET550/15×; Chroma, VT, USA) to avoid spectral overlaps. The three quasi-parallel beams are combined by three dichroic mirrors (T425LPXR, T505LPXR, and 59004bs; Chroma, VT, USA). An optimized beam expander system that integrates one divergent lens (ACN254-040-A, f = −40 mm, Thorlabs, NJ, USA) and two convergent lenses (AC508-100-A, f = 100 mm, Thorlabs, NJ, USA) is used to clearly refocus the lights at a distance of 120 mm onto the sample (see the electronic supplementary material).