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Phz1174

Manufactured by Thermo Fisher Scientific
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The PHZ1174 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for measuring pH and related parameters. The core function of this device is to provide precise and reliable pH measurement capabilities for various scientific and industrial applications.

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Lab products found in correlation

Incomplete freund s adjuvant, by BD (1 mentions) Female c57bl 6j mice, by Charles River Laboratories (1 mentions) Mog35 55, by GenScript (1 mentions) Sucrose, by Merck Group (1 mentions) Mycobacterium tuberculosis h37ra, by BD (1 mentions) Mog35 55 peptide, by GenScript (1 mentions)

2 protocols using phz1174

1

Induction and Analysis of EAE in Mice

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Young (3–4 m) and aged (15 m) old C57BL/6J female mice were acquired from Charles River Laboratories. Anesthetised mice were immunised with subcutaneous injections of 150 µg of MOG35-55 (GenScript) emulsified in 200 µl of complete Freud’s adjuvant (CFA) (Incomplete Freund’s Adjuvant containing 2.5 mg/mL Mycobacterium tuberculosis H37Ra, both BD Biosciences). At 0- and 2-days post-immunisation, mice were injected with 200 ng of pertussis toxin intraperitoneally (PHZ1174, Thermofisher). EAE clinical signs and weight were monitored daily from 7 days post-immunisation. Mice were euthanised at 36 days post-immunisation by transcardial perfusion with PBS followed by 4% PFA. Spinal cords were dissected and immersed overnight in 4% PFA at 4 °C. Next, spinal cords were cryoprotected with 30% sucrose (Sigma-Aldrich) in PBS for 72 h and snap-frozen in OCT (Tissue-Tek). Frozen spinal cords were cryosectioned at 12 µm thickness and immunostained as described below.
de la Fuente A.G., Dittmer M., Heesbeen E.J., de la Vega Gallardo N., White J.A., Young A., McColgan T., Dashwood A., Mayne K., Cabeza-Fernández S., Falconer J., Rodriguez-Baena F.J., McMurran C.E., Inayatullah M., Rawji K.S., Franklin R.J., Dooley J., Liston A., Ingram R.J., Tiwari V.K., Penalva R., Dombrowski Y, & Fitzgerald D.C. (2024). Ageing impairs the regenerative capacity of regulatory T cells in mouse central nervous system remyelination. Nature Communications, 15, 1870.
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2

Murine EAE Induction and Scoring

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EAE induction was performed as described previously with minor modifications48 (link),49 (link). All of the animal experiments were approved by the Committee for Use of Live Animals in Teaching and Research at The University of Hong Kong. 7- to 8-week-old female C57BL/6J mice were used for EAE induction. The animals were housed in the Laboratory Animal Unit on a 12 h day/night cycle, with food and water ad libitum, and were allowed to acclimatize for 1 week before disease induction.
Female mice were subcutaneously immunized with 200 μg MOG35–55 peptide (Genscript, Piscataway, NJ) in complete Freund’s adjuvant (3 mg/ml). Freshly prepared pertussis toxin (PHZ1174, ThermoFisher, 250 ng) in sterile PBS was injected intraperitoneally on day 0 and 48 h later.
EAE symptoms were scored daily as follows: 0, no clinical signs; 0.5, partially limp tail; 1, paralyzed tail; 1.5, hind limb paresis or loss in coordinated movement; 2, loss in coordinated movement and hind limb paresis; 3, one hind limb paralyzed; 4, both hind limbs paralyzed; 5, hind limbs paralyzed, weakness in forelimbs; 6, moribund.
Zhou T., Zheng Y., Sun L., Badea S.R., Jin Y., Liu Y., Rolfe A.J., Sun H., Wang X., Cheng Z., Huang Z., Zhao N., Sun X., Li J., Fan J., Lee C., Megraw T.L., Wu W., Wang G, & Ren Y. (2019). Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury. Nature neuroscience, 22(3), 421-435.
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