The HC/HT co-loaded NPs with optimized physicochemical characteristics were prepared according to Hussain et al. [3] (link). A volume of 25 mL of CS solution (0.2% w/v, prepared in 1% v/v acetic acid, pH 5.0) was incubated with HC and HT (1 mg/mL of each in 30∶70 ratio of ethanol/water) for 30 min. Co-loaded NPs were spontaneously formed by adding 10 mL of pentasodium tripolyphosphate (TPP) solution (0.1% w/v, dissolved in distilled water) dropwise under constant magnetic stirring (700 rpm). The resulting NPs were harvested by ultracentrifugation (28,000 rpm) for 30 min using an Optima L-100 XP Ultracentrifuge with an NV 70.1 Ti rotor (Beckman-Coulter, USA). Pellets of co-loaded NPs were subsequently lyophilized (Scanvac CoolSafe, Chemoscience, Thailand) at −40°C for 24 h.
Nv 70.1 ti rotor
Manufactured by Beckman Coulter
Sourced in United States
The NV 70.1 Ti rotor is a centrifuge rotor designed for use with Beckman Coulter ultracentrifuges. It is constructed of titanium and is capable of reaching high rotational speeds to facilitate the separation and analysis of samples.
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Lab products found in correlation
2 protocols using nv 70.1 ti rotor
1
Optimized Co-loaded Nanoparticle Preparation
2
Preparation of Hydrocortisone-loaded Chitosan Nanoparticles
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HC-loaded CS-NPs were prepared according to the method described by Hussain et al.17 (link) CS solution (25 mL, 0.2% w/v, prepared in 1% v/v acetic acid, pH 5.0) was incubated with HC solution (1 mg/mL in a 30:70 mixture of ethanol/water) with stirring for 30 minutes. HC-loaded CS-NPs were spontaneously formed by adding 10 mL of pentasodium tripolyphosphate solution (0.1% w/v, in distilled water) dropwise with constant stirring at 700 rpm. The resulting HC-loaded CS-NPs were harvested by ultracentrifugation (28,000 rpm) for 30 minutes using an Optima L-100 XP Ultracentrifuge with an NV 70.1 Ti rotor (Beckman-Coulter, Brea, CA, USA). The HC-loaded CS-NPs were subsequently resuspended in distilled water and lyophilized (Scanvac CoolSafe™; Chemoscience, Bangkok, Thailand) at −40°C for 24 hours.
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