H9c2 cells were plated in 6-well plates to form a monolayer on the day before the transfection. After 30–50% confluence, H9c2 cells were transfected with a scrambled siRNA or Cav-3 siRNA (100 nM; Santa Cruz Biotechnology, Santa Cruz, CA) for 6 h by using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocols [24 ]. The transfected cells were then washed twice with sterile PBS, changed with complete media and used for further experiments.
Cav 3 sirna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Cav-3 siRNA is a laboratory tool designed for gene silencing experiments. It targets the Caveolin-3 (Cav-3) gene, which is involved in the formation of caveolae, specialized membrane invaginations. This siRNA can be used to temporarily reduce the expression of the Cav-3 gene in cell culture models.
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Lab products found in correlation
4 protocols using cav 3 sirna
1
Caveolin-3 Silencing in H9c2 Cells
2
Cardioprotective Effects of Pharmacological Interventions
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Embryonic rat cardiomyocyte-derived H9C2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS in a humidified atmosphere (5% CO2) at 37°C. Commercial Cav-3 siRNA, Akt siRNA, and STAT3 siRNA (Santa Cruz Biotechnology) were used for inhibiting Cav-3, Akt, and STAT3 protein expression, respectively, according to the manufacturer's instructions. After transfection with control, Cav-3 siRNA, Akt siRNA, or STAT3 siRNA, cells were treated with N-acetylcysteine (NAC, 1 mmol/L [29 (link)]) or combination with remifentanil (2.5 μM [12 (link)]) in HG condition for 36 h; then, the cells were exposed to H/R stimulation.
3
Caveolin-3 Knockdown in HL-1 Cells
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CAV3 knockdown in HL‐1 cells was performed using cav3 siRNA (Santa Cruz) and lipofectamine 2000 (Invitrogen) as per manufacturer's protocol. A scrambled nontargeting siRNA was used as control (Santa Cruz). Prior to transfection, HL‐1 cells were placed in antibiotic‐free growth medium. At 30–50% confluency, 100 pmol siRNA were added to each well and incubated at 37°C for 20 h. Verification of Cav3 knockdown was determined by western blotting.
4
Rat Cardiomyocyte Protection against Hypoxia-Reoxygenation Injury
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Embryonic rat cardiomyocyte-derived cell line H9C2 was maintained in DMEM (Dulbecco’s modified Eagle’s medium) containing 10 % FBS in a humidified atmosphere (5 % CO2) at 37 °C. Commercial Cav-3 siRNA or eNOS siRNA (Santa Cruz Biotechnology) was used for inhibition of Cav-3 expression according to the manufacturer’s protocol. After transfection with control, Cav-3 siRNA or eNOS siRNA, cells were treated with N-acetylcysteine (NAC, 1 mmol/L) in the condition of LG or HG for 36 h, then the cells was exposed to 4 h of hypoxia (1 % O2, 5 % CO2, 94 % N2) followed by 4 h of reoxygenation (H/R). LDH (lactate dehydrogenase) release was measured to evaluate the extent of cellular injury using a cytotoxicity assay kit (Jiancheng Bioengineering Institute, China) according to the manufacturer’s instructions.
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