Immunolabeled chromosome spreads and DAPI-stained diakinesis/metaphase I nuclei were imaged using a Zeiss AxioPlan II microscope with 63× Plan Apochromat 1.4 objective and EXFO X-Cite metal halide light source. Images were captured by a Hamamatsu ORCA-ER CCD camera and processed using Volocity (Perkin Elmer) and Photoshop (Adobe) software packages. SIM analysis was performed using a Nikon N-SIM super-resolution microscope system and NIS-Elements 2 image processing software. MSH4-RNF212 colocalization was determined using NIS-Elements and co-foci were confirmed by visual inspection. Testes sections were imaged using an Axiovert 200 microscope and AxioCamMRc camera using AxioVision 4.4 software. Apoptotic cells were imaged and counted in representative fields of view.
Orca er ccd camera
Manufactured by PerkinElmer
The ORCA-ER CCD camera is a high-performance charge-coupled device (CCD) camera designed for scientific and industrial applications. It features a high-resolution, low-noise CCD sensor that delivers accurate and reliable imaging data. The camera is optimized for a wide range of applications, including microscopy, spectroscopy, and scientific imaging.
Automatically generated - may contain errors
Lab products found in correlation
N sim super resolution microscope system, by National Instruments (2 mentions)
Axiocam mrc camera, by Zeiss (2 mentions)
Volocity, by PerkinElmer (2 mentions)
Ultraview scanner, by PerkinElmer (2 mentions)
Dmi4000b microscope, by Leica (1 mentions)
Dm6000b, by Leica (1 mentions)
Las af software, by Leica (1 mentions)
Ix70 microscope, by Hamamatsu Photonics (1 mentions)
Tcs spe confocal system, by Leica (1 mentions)
Dfc350 fx ccd camera, by Leica (1 mentions)
Af7000, by Hamamatsu Photonics (1 mentions)
Em ccd c9100 camera, by Leica (1 mentions)
Af7000, by Leica (1 mentions)
Mitobright red, by Dojindo Laboratories (1 mentions)
4 protocols using orca er ccd camera
1
Imaging and Analysis of Meiotic Chromosome Spreads
2
Imaging and Analysis of Meiotic Chromosome Spreads
3
Multimodal Microscopy for Live-Cell Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Laser-scanning confocal microscopy was performed using a Leica DMI4000B microscope, with laser scanning and detection achieved by a Leica TCS SPE confocal system controlled with Leica LAS AF software. Fluorescence and wide-field microscopy was performed using a Leica DM6000B with a Leica DFC350 FX CCD camera and a Leica AF7000 with a Hamamatsu EM CCD C9100 camera and Leica LAS AF software. Leica AF7000 was also used for imaging of axon and growth cone regeneration after axotomy. Live confocal imaging was performed with an Olympus IX70 microscope using a Hamamatsu ORCA-ER CCD camera and a PerkinElmer UltraVIEW scanner for spinning disc confocal microscopy, controlled with MetaMorph software.
4
Visualizing Mitochondrial Dynamics in Astrocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human or mouse primary astrocytes were plated on either geltrex-coated 8-well chambers (Ibidi, 80826) or on CELLview glass-bottom Petri dishes (Greiner Bio One, 627860). Cells were incubated in 500 nM MitoBright Red (a membrane potential independent dye; Dojindo, MT11; gift from Dr András Füredi) diluted in culture media and incubated for 30 min at 37 °C. Media was changed before imaging to Neurobasal without phenol and with 1% Glutamax. Images were taken by a spinning-disc confocal microscope (Olympus IX70 microscope, Hamamatsu ORCA-ER CCD camera, PerkinElmer UltraVIEW scanner controlled by the MetaMorph software) at 1 second intervals. For tracking mitochondrial movements, the TrackMate ImageJ plugin was used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!