Goat anti mouse or rabbit igg peroxidase conjugated secondary antibodies

DMSO, transresveratrol (RES), curcumin from Curcuma Longa (CUR), Sulforhodamine B (SRB), staurosporine and Hoechst 33342 were purchased from Sigma-Aldrich (Milan, Italy). Rabbit polyclonal anti-Bax and mouse monoclonal anti-Bcl-2 antibodies were obtained from BD Pharmingen (BD Biosciences, San Jose, CA, USA). Antibodies against PARP-1, ERK1/2 (C-14), phospho-ERK (E-4), NF-κB and p53 (DO-1) were obtained from Santa Cruz Biotechnology (CA, USA). Rabbit polyclonal anti-actin was purchased from Sigma-Aldrich (Milan, Italy). The anti-activated caspase 3, anti-caspase 9, anti-caspase 8, anti-AKT and anti-phospho-AKT antibodies were purchased from Cell Signaling Technology (MA, USA). Antibody against LC3 was obtained from Novus Biologicals (Littleton, CO, USA). Goat anti-mouse IgG Alexa fluor-488-conjugated antibody was purchased from Life Technologies™ Molecular Probes (Oregon, USA). The goat anti-mouse or -rabbit IgG peroxidase conjugated secondary antibodies were obtained from Sigma-Aldrich (Milan, Italy).

DMSO, Sulforhodamine B (SRB), Hoechst 33342 and Pristane (2,6,10,14-Tetramethylpentadecane) were purchased from Sigma-Aldrich (Milan, Italy). (-)-gossypol (AT-101) was provided from Selleck Chemical (Munich, Germany). Z-VAD-FMK was purchased from Calbiochem (San Diego, CA, United States). Antibodies against AKT, phospho-AKT, Bax, Bcl-2, JNK/SAPK1, JNK/SAPK (pT183/pY185), p38a/SAPK2a, and p38 MAPK (pT180/pY182) were obtained from BD Pharmingen (BD Biosciences, San Jose, CA, United States). Antibodies against caspase 9, caspase 8, activated caspase 3, c-Jun, phospho-c-Jun, were obtained from Cell Signaling Technology (MA, United States). ERK1/2 (C-14), phospho-ERK (E-4), p53 (DO-1) and PARP-1 (F-2) were obtained from Santa Cruz Biotechnology (CA, United States). Anti-ErbB2 and anti-EGFR antisera were provided by Dr. M. H. Kraus (University of Alabama, Birmingham, AL, United States). Antibodies against Beclin-1 and p62/SQSTM1 were obtained from Abcam (Cambridge, United Kingdom) and the anti-LC3 antibody was purchased from Novus Biologicals (Littleton, CO, United States). The goat anti-rabbit IgG Alexa fluor-594-conjugated secondary antibody was from Invitrogen (Milan, Italy). Rabbit polyclonal antibody against actin and tubulin and the goat anti-mouse or -rabbit IgG peroxidase conjugated secondary antibodies were from Sigma-Aldrich.

DMSO and Sulforhodamine B (SRB) were purchased from Sigma-Aldrich (Milan, Italy). Bortezomib was purchased from Selleck Chemical (Munich, Germany). Z-VAD-FMK was obtained from Calbiochem (San Diego, CA, USA). Antibodies against Bax, Bcl-2, AKT, phospho-AKT, JNK/SAPK1, JNK/SAPK (pT183/pY185), p38a/SAPK2a, and p38 MAPK (pT180/pY182) were obtained from BD Pharmingen (BD Biosciences, San Jose, CA, USA). Antibodies against caspase 9, caspase 8, activated caspase 3 were obtained from Cell Signaling Technology (MA, United States). Antibodies against PARP-1 (F-2), ERK1/2 (C-14) and phospho-ERK (E-4) were obtained from Santa Cruz Biotechnology (CA, USA). Anti-ErbB2 and anti-EGFR antisera were provided by Dr. M. H. Kraus (University of Alabama, Birmingham, AL, USA). Antibodies against Beclin-1 and p62/SQSTM1 were obtained from Abcam (Cambridge, United Kingdom) and the anti-LC3 antibody was purchased from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti-actin, anti-tubulin, and the goat anti-mouse or -rabbit IgG peroxidase conjugated secondary antibodies were obtained from Sigma-Aldrich (Milan, Italy).