Syntaxin 1

The following antibodies were used: Actin (Sigma-Aldrich, St. Louis, MO, USA, #A4700 1:2500), Actin (Sigma-Aldrich, #A5441 1:2500), LC3 (Cell Signaling Technology, Danvers, MA, USA #3868 1:1000), Mfn2 (Abcam, Cambridge, UK, #Ab56889 1:1000), Neuropeptide Y (Cell Signaling Technology, #11976T 1:500), pSer293-PDH (Merck Millipore, Burlington, MA, USA #ABS204 1:1000), PDH (Santa Cruz, Dallas, TX, USA #sc-377,092), SNAP25 (Biolegend, San Diego, CA, USA #850301 1:1000), Synapsin-1 (Cell Signaling Technology, #5297T 1:1000), Synaptophysin (Abcam,#Ab14692 1:1000), Synaptotagmin-1 (Novus Biologicals, Littleton, CO, USA #MAB4364-SP 1:500), Syntaxin-1 (Sigma-Aldrich, #S0664) 1:500), TIM23 (BD Biosciences, Franklin Lakes, NJ, USA #611223 1:1000), and VDAC1 (Abcam, #Ab14734 1:1000).

We used the following antibodies at the titer indicated: VGLUT1 (1:2000), VGLUT2 (1:1000), VGAT (1:2000), SV2 (Developmental Studies Hybridoma Bank, 1:1000), synaptophysin (Sigma, 1:2000), synaptotagmin 1 (Synaptic Systems, 1:1000), VAMP2 (Synaptic Systems, 1:1000), syntaxin 1 (Sigma, 1:1000), SNAP25 (Synaptic Systems, 1:1000), munc18 (BD Biosciences, 1:500), GluR1 (Millipore 1:1000), GluR2 (Millipore, 1:500), NR1 (BD Biosciences, 1:1000), PSD-95 (Neuromab, 1:1000), TfR (Invitrogen, 1:500), rab3 (Synaptic Systems, 1:500), rab5 (Synaptic Systems, 1:500), rab7 (Sigma, 1:500), LAMP1 (Developmental Studies Hybridoma Bank, 1:500), V-ATPase H subunit (Santa Cruz, 1:100), Na⁺/K⁺ pump (Abcam, 1:1000), α-ATP synthase (Abcam, 1:1000), GFAP (Zymed, 1:250), CPE-c term (P. Loh, at 1:500), actin (Millipore, 1:1000), FLAG (Sigma, 1:2000), voltage-gated calcium channel subunit α₁A (Synaptic Systems, 1:500), voltage-gated calcium channel subunit α₂δ-1 (Sigma, 1:1000), EAAT1 (Synaptic Systems, 1:1000) and PrPc (S. Prusiner D13, 1:1000). Infrared dye-conjugated secondary antibodies (LI-COR) were used for western blotting at 1:50,000 with the exception of the calcium channel subunit α₁A and PrPc, which required chemiluminescence detection using secondary antibody conjugated to horseradish peroxidase at 1:5000 (GE Healthcare).