All specimens were scanned on a 7-Tesla small animal imaging system equipped with an Agilent VnmrJ 4 console. A custom silver solenoid coil (d =13 mm) was used for RF transmission and reception. MR-DTI images were acquired using a 3D diffusion-weighted spin-echo sequence with repetition time (TR)=100 ms, echo time (TE)=14 ms, and b-value=1600 s/mm2. The image array size was 400×200×160, over a 20.0×10.0×8.0 mm field of view, producing 50 μm isotropic image resolution. The diffusion sampling protocol included 6 diffusion directions (Jiang and Johnson, 2010 (link)) and 1 non-diffusion-weighted (b0) measurement. Total acquisition time was 7 hours. After registering all individual DWI images (each sensitized to a different diffusion direction) to the b0 image with an affine transform we used the Diffusion Toolkit (Wang et al., 2007 ) to estimate the diffusion tensor and calculate the mean diffusion-weighted image (DWI), axial diffusivity (AD), fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and apparent diffusion coefficient (ADC). The DTI parametric images were used as the input for the VBA pipeline.
Vnmrj 4 console
Manufactured by Agilent Technologies
The VnmrJ 4 console is a nuclear magnetic resonance (NMR) spectrometer software package developed by Agilent Technologies. It provides the user interface and control functions for Agilent's NMR instrumentation, enabling researchers to acquire, process, and analyze NMR data.
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Lab products found in correlation
3 protocols using vnmrj 4 console
1
High-resolution Diffusion Tensor Imaging of Small Animal Specimens
2
3D Diffusion MRI of Small Animal Brain
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Imaging was performed on a 9.4-Tesla small animal imaging system controlled by an Agilent VnmrJ 4 console. A custom-made silver solenoid coil was used for radiofrequency transmission and reception. Diffusion imaging was accomplished using a 3D diffusion-weighted spin-echo pulse sequence with repetition time (TR) = 100 ms, echo time (TE) = 15 ms, and b-value = 4000 s/mm2. The acquisition matrix was 568 × 284 × 228 over a 24.4 mm × 12.2 mm × 9.8 mm field of view, resulting in a native isotropic image resolution of 43 µm. The diffusion sampling protocol included 120 unique diffusion directions (Koay 2011 (link); Koay et al. 2011 (link)) and 11 nondiffusion-weighted (b0) measurements (i.e., one b0 every 12 diffusion measurements). Total acquisition time was 235 h. Bore temperature was controlled with a chilled water pump and monitored with a fiber-optic probe for the duration of the experiment (Supplementary Fig. 1 ).
3
High-Resolution Diffusion Tensor Imaging
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All specimens were scanned on a 7-Tesla small animal imaging system equipped with an Agilent VnmrJ 4 console. A custom silver solenoid coil (d = 13 mm) was used for RF transmission and reception. MR-DTI images were acquired using a 3D diffusion-weighted spin-echo sequence with repetition time (TR) = 100 ms, echo time (TE) = 14 ms, and b-value = 1600 s/mm2. The image array size was 400x200x160, over a 20.0 × 10.0 × 8.0 mm field of view, producing 50 μm isotropic image resolution. The diffusion sampling protocol included 6 diffusion directions (Jiang and Johnson 2010 (link)) and 1 non-diffusion-weighted (b0) measurement. Total acquisition time was 7 h. After registering all individual DWI images (each sensitized to a different diffusion direction) to the b0 image with an affine transform we used the Diffusion Toolkit (Wang et al. 2007 ) to estimate the diffusion tensor and calculate the mean diffusion-weighted image (DWI), axial diffusivity (AD), fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and apparent diffusion coefficient (ADC). The DTI parametric images were used as the input for the VBA pipeline.
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