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Metamorph 6.1 software system

Manufactured by Universal Imaging
Sourced in United States

MetaMorph 6.1 Software System is a comprehensive software package designed for image acquisition, analysis, and automation in microscopy applications. It provides a robust and flexible platform for managing various imaging data and workflows.

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Lab products found in correlation

2 protocols using metamorph 6.1 software system

1

Morphometric Analysis of Vesicles

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MetaMorph 6.1 Software System (Universal Imaging Corp, Molecular Device Corp, CA, USA) was employed to acquire digital images and perform morphometric analysis, as already described40
. Morphometric computerized analysis of about 100 MV mean diameters and the specific vesicle areas and diameters (10 photographic fields at 7,000×; 12,000×; 20,000×; and 50,000× on five sections) were performed manually drawing the area regions and the diameter segments, after calibrating the MetaMorph 6.1r6 program (Universal Imaging Corp) for the magnification used (7,000×; 12,000×; 20,000×; and 50,000×).
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2

Microscopic Analysis of Stained Cell Samples

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The observation of samples processed for Immunofluorescence, Alkaline Phosphate, Haematoxylin–Eosin and Mallory’s trichrome staining solution was carried out with a Zeiss Axioskop 40 light microscope (Carl Zeiss) equipped with a Coolsnap Videocamera (Photometrics). MetaMorph 6.1 Software System (Universal Imaging Corp, Molecular Device Corp, CA, USA) was employed to acquire digital images and perform morphometric analysis, as already described (Antonucci et al. 2014 (link)). Morphometric computerized analysis of about 100 EBs mean diameters and the specific vesicle areas and diameters (10 photographic fields at 20,000 × on 5 sections) were performed manually drawing the area regions and the diameter segments, after calibrating the MetaMorph 6.1r6 program (Universal Imaging Corp) for the magnification used (5 ×; 20000 ×). MetaMorph numeric data were logged to Microsoft Excel to calculate mean values and standard deviation (SD).
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