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Nova lite hep 2

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The Nova Lite Hep-2 is an automated indirect immunofluorescence assay system designed for the detection of antinuclear antibodies (ANA) in human serum or plasma samples. The system utilizes Hep-2 cells as the substrate for ANA detection and provides automated slide processing, image acquisition, and result interpretation.

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Lab products found in correlation

Fv1000 tirf, by Olympus (2 mentions) Ab11433, by Abcam (1 mentions) Nova view, by Inova Diagnostics (1 mentions)

3 protocols using nova lite hep 2

1

HEp-2 Indirect Fluorescence Assay

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A HEp‐2 IFA (NOVA Lite HEp‐2, Inova Diagnostics) was used to detect anticellular antibodies at a serum dilution of 1:80, which were read on an automated instrument (NovaView, Inova Diagnostics) that interpolated fluorescence intensity to an end‐point titer. A commercially available monoclonal anti‐VCP antibody (Abcam, Cat. # ab11433; immunogen: synthetic peptide corresponding to human aa 792‐806) was used as a reference for IFA staining.
Amlani A., Choi M.Y., Buhler K.A., Hudson M., Tarnopolsky M., Brady L., Schmeling H., Swain M.G., Stingl C., Reed A, & Fritzler M.J. (2022). Anti–Valosin‐Containing Protein (VCP/p97) Autoantibodies in Inclusion Body Myositis and Other Inflammatory Myopathies. ACR Open Rheumatology, 5(1), 10-14.
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2

Immunofluorescence Assay for Antinuclear Antibodies

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Hep-2–coated slides (Nova lite Hep-2, catalog number 708101; INOVA Diagnostics) were incubated in a moist chamber at ambient temperature with 20 μl purified Abs at 30 μg/ml for 30 min, washed in PBS, and incubated for 30 min with FITC-labeled goat anti-human IgG (20 μl). ANA-negative and ANA-positive control sera were included in all experiments. Samples were examined on a confocal microscope, Olympus FV1000/TIRF (Emory University Integrated Cellular Imaging Microscopy Core). Positive staining was determined by comparison with the controls at equal exposure times.
Tipton C.M., Fucile C.F., Darce J., Chida A., Ichikawa T., Gregoretti I., Schieferl S., Hom J., Jenks S., Feldman R.J., Mehr R., Wei C., Lee F.E., Cheung W.C., Rosenberg A.F, & Sanz I. (2015). Diversity, cellular origin and autoreactivity of antibody-secreting cell expansions in acute Systemic Lupus Erythematosus. Nature immunology, 16(7), 755-765.
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3

Immunofluorescence Assay for Antinuclear Antibodies

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Hep-2–coated slides (Nova lite Hep-2, catalog number 708101; INOVA Diagnostics) were incubated in a moist chamber at ambient temperature with 20 μl purified Abs at 30 μg/ml for 30 min, washed in PBS, and incubated for 30 min with FITC-labeled goat anti-human IgG (20 μl). ANA-negative and ANA-positive control sera were included in all experiments. Samples were examined on a confocal microscope, Olympus FV1000/TIRF (Emory University Integrated Cellular Imaging Microscopy Core). Positive staining was determined by comparison with the controls at equal exposure times.
Tipton C.M., Fucile C.F., Darce J., Chida A., Ichikawa T., Gregoretti I., Schieferl S., Hom J., Jenks S., Feldman R.J., Mehr R., Wei C., Lee F.E., Cheung W.C., Rosenberg A.F, & Sanz I. (2015). Diversity, cellular origin and autoreactivity of antibody-secreting cell expansions in acute Systemic Lupus Erythematosus. Nature immunology, 16(7), 755-765.
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