Probnp 2 stat

Manufactured by Roche

Sourced in Germany

The ProBNP II STAT is a laboratory assay designed to quantitatively measure N-terminal pro-B-type natriuretic peptide (NT-proBNP) in human plasma or serum samples. NT-proBNP is a biomarker used in the clinical assessment of heart failure.

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Lab products found in correlation

Cobas e602 analyzer, by Roche (4 mentions) Architect stathigh sensitive troponin 1, by Abbott (2 mentions) Architect i2000sr analyzer, by Abbott (2 mentions) Electrochemiluminescence immunoassay, by Roche (1 mentions) Human gdf 15 quantikine elisa kit, by R&D Systems (1 mentions)

5 protocols using probnp 2 stat

Serum Biomarker Measurement Protocol

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All biochemical analyses were performed in a single freeze–thaw cycle >3 assay runs using the same lot of reagents in an accredited laboratory (Changi General Hospital, Singapore). Serum NT-proBNP (proBNP II STAT; Roche Diagnostics, Pensberg, Germany) and hsTnT (STAT; Roche Diagnostics, Pensberg, Germany) were assayed using electrochemiluminescence immunoassay on the Cobas E602 analyzer (Roche Diagnostics Asia-Pacific, Singapore). Serum hsTnI (ARCHITECT STAT High-Sensitive Troponin-I; Abbott Diagnostics, Abbott Park, IL) and galectin-3 (ARCHITECT Galectin-3; Abbott Diagnostics) were determined using chemiluminescent microparticle immunoassay on the ARCHITECT i2000SR analyzer (Abbott Laboratories [Diagnostics], Singapore). Blood samples were collected on the day of CMR.
The manufacturer-reported lower limit of detection (LOD) and the 99th percentile upper reference limit for NT-proBNP were 5 and 135 pg/mL, respectively.³¹ The LOD and 99th percentile upper reference limit for hsTnT were 5 and 14 pg/mL, respectively.³² The LOD for hsTnI was 1.1 ng/L. We have determined previously that the 99th percentile upper reference limit for hsTnI was 26 ng/L.^{33 (link)} Serum galectin-3 had a manufacturer-reported LOD of 1.0 ng/mL.³⁴ All biochemical concentrations lower than the detection levels were assigned a value equivalent to half the LOD.

Goh V.J., Le T.T., Bryant J., Wong J.I., Su B., Lee C.H., Pua C.J., Sim C.P., Ang B., Aw T.C., Cook S.A, & Chin C.W. (2017). Novel Index of Maladaptive Myocardial Remodeling in Hypertension. Circulation. Cardiovascular Imaging, 10(9), e006840.

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Cardiac Biomarkers Measurement Protocol

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Serum N-terminal pro-B-type natriuretic peptide (NT-proBNP; proBNP II STAT, Roche Diagnostics, Pensberg, Germany) was assayed using electrochemiluminescence immunoassay on the Cobas E602 analyzer (Roche Diagnostics Asia-Pacific, Singapore). Serum high-sensitive cardiac troponin I (hsTnI; ARCHITECT STAT high-sensitive Troponin-I; Abbott Diagnostics, Abbott Park, IL) was determined using chemiluminescent microparticle immunoassay on the ARCHITECT i2000SR analyzer (Abbott Laboratories, Singapore). The lower limit of detection (LOD) for NT-proBNP and hsTnI was 5 pg/mL and 1.1 ng/L, respectively.²² ^,²³ All biochemical concentrations lower than the detection levels were assigned a value equivalent to half the LOD.

Le T.T., Lim V., Ibrahim R., Teo M.T., Bryant J., Ang B., Su B., Aw T.C., Lee C.H., Bax J., Cook S, & Chin C.W. (2020). The remodelling index risk stratifies patients with hypertensive left ventricular hypertrophy. European Heart Journal Cardiovascular Imaging, 22(6), 670-679.

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Preoperative Biomarkers Profiling

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Preoperative GDF15 circulating levels (in pg/mL) were assessed on plasma samples from patients and control subjects with a sandwich immunoassay (ElectroChemiLuminescence ImmunoAssay, ECLIA—Elecsys, Roche, Basel, Switzerland), according to the manufacturer’s instructions. High-sensitivity Troponin T (hs-TnT STAT, Roche, Basel, Switzerland) and N-terminal pro-brain natriuretic peptide (proBNP II STAT, Roche, Basel, Switzerland) were also assessed. Renal function (estimated glomerular filtration rate, eGFR) was estimated with the Modification of Diet in Renal Disease (MDRD) formula. Biomarkers levels were normalized (log₁₀) for statistical analysis when appropriate.

Fabiani I., Santoni T., Angelillis M., Petricciuolo S., Colli A., Pellegrini G., Mazzei D., Pugliese N.R., Petronio A.S, & De Caterina R. (2020). Growth Differentiation Factor 15 in Severe Aortic Valve Stenosis: Relationship with Left Ventricular Remodeling and Frailty. Journal of Clinical Medicine, 9(9), 2998.

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Serum Biomarkers in Cardiovascular Disease

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Blood samples were collected on the day of CMR and stored at −80 °C. Serum NT-proBNP (N-terminal pro-B-type natriuretic peptide; proBNP II STAT, Roche Diagnostics, Pensberg, Germany) was assayed using electrochemiluminescence immunoassay on the Cobas E602 analyzer (Roche Diagnostics Asia-Pacific, Singapore). The manufacturer reported lower limit of detection was 10 pg/mL. Concentrations lower than the detection levels in the participants were assigned a value equivalent to half the limit of detection. Serum GDF-15 (growth differentiation factor 15) concentration was measured using Human GDF-15 Quantikine ELISA Kit (DGD150; R&D Systems, Minneapolis) in duplicates according to the manufacturer’s protocol. The manufacturer reported minimum detectable dose of human GDF-15 was between 0.0 and 4.4 pg/mL (mean, 2.0 pg/mL).

Pua C.J., Loo G., Kui M., Moy W.L., Hii A.A., Lee V., Chin C.T., Bryant J.A., Toh D.F., Lee C.H., Cook S.A., Richards A.M., Le T.T, & Chin C.W. (2023). Impact of Diabetes on Myocardial Fibrosis in Patients With Hypertension: The REMODEL Study. Circulation. Cardiovascular Imaging, 16(7), 545-553.

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Cardiac Biomarkers in CMR Evaluation

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Blood samples were collected on the day of CMR and stored at −80 °C. Biochemical analyses were performed in a single freeze-thaw cycle over 2 assay runs at a laboratory accredited by the College of American Pathologists (Changi General Hospital, Singapore). Serum NT-proBNP (N-terminal pro-B-type natriuretic peptide; proBNP II STAT; Roche Diagnostics, Pensberg, Germany) was assayed using electrochemiluminescence immunoassay on the Cobas E602 analyzer (Roche Diagnostics Asia Pacific, Singapore). Serum high-sensitivity cardiac troponin I (hsTnI; ARCHITECT STAT high-sensitivity troponin I; Abbott Diagnostics, Abbott Park, IL) was determined using chemiluminescent microparticle immunoassay on the ARCHITECT i2000SR analyzer (Abbott Laboratories, Singapore). The manufacturer-reported lower limit of detection NT-proBNP and hsTnI was 5 pg/mL and 1.1 ng/L, respectively.^{25 ,26} All biochemical concentrations lower than the detection levels in the participants were assigned a value equivalent to half the limit of detection.

Iyer N.R., Le T.T., Kui M.S., Tang H.C., Chin C.T., Phua S.K., Bryant J.A., Pua C.J., Ang B., Toh D.F., Aw T.C., Lee C.H., Cook S.A., Ugander M, & Chin C.W. (2022). Markers of Focal and Diffuse Nonischemic Myocardial Fibrosis Are Associated With Adverse Cardiac Remodeling and Prognosis in Patients With Hypertension: The REMODEL Study. Hypertension (Dallas, Tex. : 1979), 79(8), 1804-1813.

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