Qgy 7703

Human HCC cell lines (QGY-7703 and Huh7) and HEK293T cells were obtained from the Shanghai Cell Bank of Chinese Academy of Sciences (Shanghai, China) and American Type Culture Collection (ATCC, USA), respectively. All these cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin at 37 °C in a humidified incubator with 5% CO₂.
For transfection, HEK293T or HCC cells at 70% confluence were transfected with plasmids by using Lipofectamine® 2000 or Lipofectamine® 3000 (Invitrogen) in a serum-free medium according to the manufacturer’s instructions. After 6 h of transfection, the medium was replaced with a fresh complete medium.

The normal human liver cell line LO2 and cancerous cell lines QGY-7703, SMMC-7721, Huh7, Hep3B, and GSG7701 were purchased from the Shanghai Cell Bank. Cells were grown in Dulbecco’s modified essential medium containing 10% fetal bovine serum (FBS) and 100 μg/ml penicillin/streptomycin. We used a special incubator containing 5% CO₂ and cultured target cells at 37°C. Female BALB/C nude mice (28–35 days old) were provided by the Animal Core Facility (Shanghai, China) and housed at 25°C under pathogen-free conditions.