Fasting serum lipids (total cholesterol, high‐density lipoprotein [HDL] cholesterol, and triglycerides) and hsCRP (Dade Behring, Deerfield, IL) were measured at the San Francisco General Hospital clinical lab. Blood was drawn in the fasting state at the San Francisco General Hospital clinical laboratories and aliquots of serum and plasma were stored at −80°C until analysis. Total cholesterol, HDL‐cholesterol, and triglycerides were measured by colorimetric assay kits (Thermo Fisher Scientific, Middletown, VA). ApoA1 and apoB were measured by nephelometric immunoassays (BNII; Siemens Healthcare Diagnostics, Deerfield, IL). Platelet‐activating factors acetylhydrolase activity (PAH) and secretory phospholipase A2 levels were measured by kits from Cayman Chemical (Ann Arbor, MI). Lecithin‐cholesterol acyltransferase, and oxidized low‐density lipoprotein levels were measured with commercially available ELISA kits from ALPCO (Salem, NH), and Mercodia (Winston Salem, NC), respectively. Paraoxonase arylesterase activity was determined by using phenyl acetate as substrate as described by Furlong et al.20 (link)Percentage of CD19 B‐cells was assessed using quantitative flow cytometry performed at the San Francisco General Hospital reference laboratory (ARUP Laboratories, test code 0095920).
Hs crp
Manufactured by Siemens
Sourced in Germany, United States
Hs-CRP is a lab equipment product that measures the concentration of C-reactive protein (CRP) in a blood sample. CRP is a biomarker that indicates the presence of inflammation in the body. The Hs-CRP device provides a quantitative measurement of CRP levels, which can be used by healthcare professionals to assess and monitor various medical conditions.
Automatically generated - may contain errors
Lab products found in correlation
Colorimetric assay kit, by Thermo Fisher Scientific (1 mentions)
Dedicated elisa kits, by R&D Systems (1 mentions)
Liatest d di, by Diagnostica Stago (1 mentions)
Hs600b, by R&D Systems (1 mentions)
Timp 1, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by Mesoscale (1 mentions)
Tnf α, by Merck Group (1 mentions)
Quantikine elisa, by R&D Systems (1 mentions)
Il 37, by Adipogen (1 mentions)
12 protocols using hs crp
1
Fasting Lipid and Inflammation Biomarkers
2
Comprehensive Cardiovascular Risk Assessment
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Blood samples were collected from the antecubital vein under fasting conditions and stored at -80° C until use. Blood count, lipid profile (TC, LDL-c, HDL-c, and TG), serum glucose, and renal and hepatic function were assessed using standard laboratory techniques. Lp(a) levels were evaluated using a standard immunochemical-based assay with reference ranges < 30 mg/dl or <75 nmol/l [16 , 17 (link)].
The LDL peak buoyancy and cholesterol distribution across the lipoprotein classes were assessed by density gradient ultracentrifugation (DGUC) for apo B containing lipoproteins, optimized for LDL particles. Thirty-seven 0.45 ml fractions were then collected from the bottom of the centrifuge tube. Cholesterol was measured in each fraction. The relative flotation rate (Rf), which characterizes LDL peak buoyancy, was obtained by dividing the fraction number containing the LDL-cholesterol peak by the total number of fractions collected. Each lipoprotein subclass elution range was defined as previously published [18 (link)]. Circulating levels of osteoprotegerin (OPG) were assessed by using dedicated ELISA kits (R&D System), whereas serum levels of high-sensitivity CRP (hs-CRP) have been measured through a sensitive immunonephelometric method (Dade Behring).
The LDL peak buoyancy and cholesterol distribution across the lipoprotein classes were assessed by density gradient ultracentrifugation (DGUC) for apo B containing lipoproteins, optimized for LDL particles. Thirty-seven 0.45 ml fractions were then collected from the bottom of the centrifuge tube. Cholesterol was measured in each fraction. The relative flotation rate (Rf), which characterizes LDL peak buoyancy, was obtained by dividing the fraction number containing the LDL-cholesterol peak by the total number of fractions collected. Each lipoprotein subclass elution range was defined as previously published [18 (link)]. Circulating levels of osteoprotegerin (OPG) were assessed by using dedicated ELISA kits (R&D System), whereas serum levels of high-sensitivity CRP (hs-CRP) have been measured through a sensitive immunonephelometric method (Dade Behring).
3
Biomarkers of HIV Progression
4
Biomarker Measurement in Fasting Samples
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Overnight fasting serum and plasma samples were obtained on the day of surgery for measurement of lipid profiles, renal function, and biomarkers. The estimated glomerular filtration rate (eGFR) was calculated by a modified Modification of Diet in Renal Disease equation based on a Chinese population using plasma creatinine (PCr, mg/dl) as eGFR (ml/min/1.73 m2) = 175 × PCr −1.234 × age −0.179 × (0.79 if the subject was a woman) (16 (link)). NT-proBNP (Biomedica, Vienna, Austria), hs-CRP (Dade Behring, Marburg, Germany), MMPs, and TIMP-1 (R&D Systems, Abingdon, United Kingdom) levels were determined using commercially available enzyme-linked immunosorbent assays.
5
Metabolic Measurements in Sleep Disorders
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Laboratory data, either collected during the same day of the polysomnographic recording or during the visit preceding the same recording (in general within 2 weeks) were abstracted from electronic clinical files from our unit. Serum lipids, glucose, albumin, phosphate, parathyroid hormone, and hemoglobin levels were measured by standard methods in the routine clinical laboratory. hs‐CRP (high‐sensitivity C‐reactive protein) was measured following the high‐sensitivity method (Dade Behring, Marburg, Germany). Serum creatinine was measured by an automated technique, according to the Jaffe chromogen method (calibrated to the isotope dilution mass spectrometry standard) implemented in an autoanalyzer. The estimated GFR (eGFR) was estimated by the modification of diet in renal disease equation developed by Levey et al.24
6
Measuring Inflammatory and Adipokine Markers
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Levels of high sensitive CRP (hs-CRP) were measured using the turbidimetric immunoassay technique (SIEMENS, Washington, DC, USA). Serum adiponectin, leptin, and resistin were measured using the human adipocyte magnetic panel kit (Millipore Sigma, Burlington, MA, USA), using Luminex xMAP® technology (Millipore Sigma) and following the manufacturer’s instructions.
7
Comprehensive Biomarker Profiling in Diabetes
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HbA1c was determined by high-performance liquid chromatography (Menarini Diagnostics, Firenze, Italy). Total serum cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol were measured using standard enzymatic methods. Estimated glomerular filtration rate (eGFR) was calculated using the Chronic Kidney Disease-Epidemiology (CKD-EPI) equation on the basis of serum creatinine, age, sex and race [18 (link)]. Serum 25(OH)D was measured using an ELISA kit (BioVendor, Brno, Czech Republic). Plasma FGF-23 was measured using an ELISA kit (Immunotopics Inc. San Clemente, CA, USA). Intra- and inter-assay coefficients of variation were 5% and 7.8% for 25(OH)D and 2.4% and 4.7% for FGF-23, respectively. hsCRP was determined by immunonephelometry (Siemens, Munich, Germany). IL-6 was determined by ELISA (R&D Systems, Oxon, UK), as well as sTNFαR1 (HyCult Biotech, Uden, The Netherlands) and sTNFαR2 (R&D Systems, Oxon, UK). Concentrations of sICAM-1, sVCAM-1 and E-selectin were also determined by ELISA (Wuhan Boster Biological Technology, Ltd., Wuhan, China) as previously described [15 (link),19 (link)].
8
Biomarker Evaluation in HFpEF Subtypes
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hs‐CRP, IL‐6,TNF‐α and PTX3 were measured at baseline exams by the NHLBI Heart Failure Research Network Biomarker Core Laboratory at the University of Vermont using commercially available kits (hs‐CRP, Siemens, Indianapolis; IL‐6, Meso Scale Discovery, Gaithersbury, MD; TNF‐α, EMD Millipore, Billerica, MA; PTX3, R&D systems, Minneapolis, MN). Samples were shipped on dry ice and stored at −80°C until analysis at the Biomarker Core Laboratory.
In addition to determining whether there are differences in pro‐inflammatory biomarkers in AD‐HFpEF versus S‐HFpEF, we also tested whether biomarker levels are associated with echocardiographic‐Doppler abnormalities of left ventricular diastolic function in all comers and are predictive of clinical outcomes in AD‐HFpEF. In the DOSE and ROSE trials short‐term clinical outcomes included urine volume, change in cystatin‐C, change in creatinine, change in NT‐proBNP, and change in weight over the 72 hours after randomization. In addition, post‐discharge clinical status with respect to survival and re‐hospitalizations was determined by telephone call 60 days after randomization for each trial.
In addition to determining whether there are differences in pro‐inflammatory biomarkers in AD‐HFpEF versus S‐HFpEF, we also tested whether biomarker levels are associated with echocardiographic‐Doppler abnormalities of left ventricular diastolic function in all comers and are predictive of clinical outcomes in AD‐HFpEF. In the DOSE and ROSE trials short‐term clinical outcomes included urine volume, change in cystatin‐C, change in creatinine, change in NT‐proBNP, and change in weight over the 72 hours after randomization. In addition, post‐discharge clinical status with respect to survival and re‐hospitalizations was determined by telephone call 60 days after randomization for each trial.
9
Assessment of Serum Pentraxin-3 and hs-CRP Levels
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Venous blood samples were collected from all subjects after PSG at 7 am using gel tubes. The blood samples were centrifuged and then stored at -80°C till measurement. Serum pentraxin-3 levels were measured by enzyme-linked immunosorbent assay (ELISA) using Quantikine® ELISA (Human Pentraxin-3 Immunoassay, R&D Systems, Inc., Minneapolis, USA). Values were expressed as ng/mL. Serum hs-CRP levels were measured with an immunonephelometric method (hsCRP, Siemens, Erlangen, Germany) and expressed as mg/dl.
10
Quantification of Inflammatory Markers
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The concentrations of plasma IL-37, OPG, and hs-CRP were measured by an enzyme-linked immunosorbent assay (ELISA) according to instructions provided by the manufacturers (IL-37: Adipogen AG, Liestal, Switzerland; OPG: MBL, Nagoya, Japan; and hs-CRP: Siemens Healthcare, Tarrytown, New York). All samples were measured in duplicate.
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