Bacteriological agar, casein peptone, and yeast extract powder were purchased from Becton Dickinson (Franklin Lakes, NJ, USA). Citric acid hydrate, D-(+)-glucose anhydrous, sodium dodecyl sulfate (SDS), sodium dihydrogen phosphate anhydrous, sodium hydroxide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and carboxymethyl cellulose (CMC, C5678) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Avicel-plus® CM 2159 was obtained from FMC Biopolymers, USA. Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS) and penicillin-streptomycin was obtained from Merck Millipore (Burlington, VT, USA).
Casein peptone
Manufactured by BD
Sourced in United States
Casein peptone is a complex mixture of peptides and amino acids derived from the enzymatic hydrolysis of casein, a protein found in milk. It serves as a nutrient source for the growth and cultivation of various microorganisms in laboratory settings.
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Lab products found in correlation
Sodium dihydrogen phosphate anhydrous, by Merck Group (1 mentions)
Bacteriological agar, by BD (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Citric acid hydrate, by Merck Group (1 mentions)
D glucose anhydrous, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
10 ml culture tubes, by Thermo Fisher Scientific (1 mentions)
Cetyltrimethylammonium bromide ctab, by Merck Group (1 mentions)
Lactic acid, by Merck Group (1 mentions)
Potato dextrose agar (pda), by BD (1 mentions)
Plate count agar, by BD (1 mentions)
L fucose, by Merck Group (1 mentions)
2 fucosyllactose, by Biosynth (1 mentions)
D lactose, by Merck Group (1 mentions)
D galactose, by Merck Group (1 mentions)
Pnpfuc, by Merck Group (1 mentions)
D glucose, by Merck Group (1 mentions)
4 protocols using casein peptone
1
Preparation of Cell Culture Media
2
Cultivation and Harvesting of Giardia Trophozoites
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Trophozoites from G. lamblia WBC6 (C6), WBA1 (A1) and GS/M-83-H7 (H7) were grown under anaerobic conditions in 10 mL culture tubes (Nunc, Roskilde, Denmark) on modified TYI-S-33 medium as previously described (Clark and Diamond, 2002). In order to ensure the growth of the A1 and H7 clones, the components were as close as possible to the isolation medium, in particular heat-inactivated adult bovine serum (Biofluids, Rockville, MD, USA) and casein peptone (Becton Dickinson, Cockeysville, MD, USA). Prior to shotgun mass spectrometry analysis, the cultures were routinely passaged two times. Subcultures were performed by inoculating 100 μL of cells from a confluent culture detached by cooling (see below) to a new tube containing 10 mL culture medium (Müller et al., 2006). Trophozoites were harvested by incubation on ice for 15 min followed by centrifugation (300 × g, 10 min, 4°C). Pellets were washed three times with ice-cold PBS, counted and stored at −80°C for subsequent proteomic analysis or for enzymatic assays.
3
Antimicrobial Oregano-based Edible Coatings
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Sodium caseinate was purchased from Fonterra Group (Auckland, the Netherland), Chitosan Powder (90% deacetylation; viscosity 50-800 mPa•s) was obtained from Chemsavers (Bluefield, VA, USA). Anhydrous glycerol was purchased from J.T. Baker (Radnor, PA, USA); lactic acid (85%), cetyltrimethylammonium bromide (CTAB) and tetraethyl orthosilicate (98%) (TeOS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fresh oregano leaves (Lippia graveolens Kunth) were collected in Cerrito Parado, Tolimán (Querétaro, México). A voucher specimen was authenticated and deposited in the Ethnobotanical Collection of the Herbarium of Querétaro "Dr. Jerzy Rzedowski" (QMEX), located at the Faculty of Natural Sciences, University of Querétaro, Mexico (voucher specimen: E. Hernández-Hernández No. 1). Potato dextrose agar, plate count agar and casein peptone were acquired from BD Bioxon (Estado de Mexico, México).
All bacteria tested were obtained from the microbial collection of the Food Biotechnology Laboratory, DIPA, Universidad Autónoma de Querétaro (Mexico). Selected bacteria were those relevant in microbial contamination of fresh cheese, Listeria innocua and Salmonella Saintpaul. The strains were stored at -60 • C in sterile glycerol. The bacteria were activated in nutrient broth (Bioxon) at 37
All bacteria tested were obtained from the microbial collection of the Food Biotechnology Laboratory, DIPA, Universidad Autónoma de Querétaro (Mexico). Selected bacteria were those relevant in microbial contamination of fresh cheese, Listeria innocua and Salmonella Saintpaul. The strains were stored at -60 • C in sterile glycerol. The bacteria were activated in nutrient broth (Bioxon) at 37
4
Enzymatic Assay for Fucosyltransferase Activity
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Yeast extract and casein peptone were purchased from B. D. Bioxon (Mexico City, Mexico). pNP-Fuc, pNP, D-lactose, D-glucose, D-galactose, L-fucose, and Man Rogosa and Sharpe (MRS) agar were purchased from Sigma-Aldrich (St. Louis, MO, USA), 2′-fucosyllactose was purchased from Carbosynth (Berkshire, UK). Sodium phosphate and sodium hydroxide were purchased from J. T. Baker (Mexico City, Mexico). Milli-Q® (Billerica, MA, USA) water was used throughout the experiments.
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