Human TNBC cell lines MDA-MB-231, MDA-MB-436, MDA-MB-157, HCC1806, HCC1937, Hs578T, HCC38, BT549, HCC1187, and HCC1395 were obtained from American Type Culture Collection (ATCC, Manassas, VA). CAL-51, CAL-85-1, CAL-120, CAL-148 and HDQ-P1 were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany). MDA-MB-468, BT20, and HCC70 were obtained from the University of Colorado Cancer Center (UCCC) Tissue Culture Core laboratory. The MCF-10A cell line was obtained as a kind gift from Traci Lyons, PhD and used as a control. All breast cancer cells were cultured in DMEM supplemented with 10% FBS, 1% penicillin–streptomycin, 1% MEM nonessential amino acids and 1% normocin. All cells were grown in an incubator at 37°C containing 5% CO2. Cell lines were routinely authenticated by the Barbara Davis Center for Childhood Diabetes Core and screened for mycoplasma every 3 months.
Cal 85 1
Manufactured by Leibniz Institute DSMZ
Sourced in Australia, Germany
The CAL-85-1 is a laboratory instrument designed for the cultivation and analysis of microbial cultures. It provides a controlled environment for the growth and monitoring of a variety of microorganisms. The core function of the CAL-85-1 is to maintain consistent temperature, humidity, and aeration conditions necessary for the cultivation of microbial samples.
Automatically generated - may contain errors
Lab products found in correlation
Cal 148, by Leibniz Institute DSMZ (12 mentions)
Cal 51, by Leibniz Institute DSMZ (6 mentions)
Mda mb 231, by American Type Culture Collection (6 mentions)
Hcc38, by American Type Culture Collection (6 mentions)
Hcc1806, by American Type Culture Collection (6 mentions)
Bt549, by American Type Culture Collection (6 mentions)
Mda mb 157, by American Type Culture Collection (6 mentions)
Hcc1937, by American Type Culture Collection (6 mentions)
Hs578t, by American Type Culture Collection (6 mentions)
Mda mb 436, by American Type Culture Collection (6 mentions)
Hcc1569, by American Type Culture Collection (5 mentions)
Mda mb 468, by American Type Culture Collection (4 mentions)
Du4475, by American Type Culture Collection (4 mentions)
Mda mb 453, by American Type Culture Collection (4 mentions)
Hcc70, by American Type Culture Collection (4 mentions)
Bt 20, by American Type Culture Collection (4 mentions)
Hcc1143, by American Type Culture Collection (4 mentions)
Hcc1954, by American Type Culture Collection (4 mentions)
Hcc1500, by American Type Culture Collection (4 mentions)
Hdq p1, by Leibniz Institute DSMZ (2 mentions)
4 protocols using cal 85 1
1
Breast Cancer Cell Line Cultivation Protocol
2
Breast Cancer Cell Line Cultures
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The BT549, BT20, DU4475, HCC38, HCC70, HCC1500, HCC1569, HCC1954, HCC1806, HCC1143, HCC1937, HS578T, MDA-MB-157, MDA-MB-436, MDA-MB-453, MDA-MB-231, and MDA-MB-468 cell lines were purchased from the American Type Culture Collection; CAL51, CAL148, and CAL851 cells were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen; SUM185PE and SUM149PT cells were purchased from Asterand Bioscience; MFM223 cells were purchased from Sigma-Aldrich; and CAL120 cells were a gift from Professor Elgene Lim from the Garvan Institute of Medical Research, Darlinghurst, NSW, Australia. All the above cell lines were cultured in RPMI supplemented with 10% FBS and 0.023 IU/ml (or 10 µg/ml) insulin. MDA-MB231(LM2) cells (a kind gift from Dr. Joan Massague, Sloan-Kettering Memorial Institute, New York, NY; Minn et al., 2005 (link)) were cultured in DMEM supplemented with 10% FBS.
3
Comprehensive Breast Cancer Cell Line Panel
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The BT549, BT20, DU4475, HCC38, HCC70, HCC1500, HCC1569, HCC1954, HCC1806, HCC1143, HCC1937, HS578T, MDA-MB-157, MDA-MB-436, MDA-MB-453, MDA-MB-231 and MDA-MB-468 cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). CAL51, CAL148 and CAL851 cells were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) and CAL120 cells were a gift from Professor Elgene Lim from the Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia. MFM223 cells were purchased from Sigma Aldrich. SUM185PE and SUM149PT cells were purchased from Asterand Bioscience. Cells were cultured in RPMI 1640 (Gibco) supplemented with 10% (v/v) FBS, 10 μg/mL insulin and 20 mM HEPES.
4
Characterization of Breast Cancer Cell Lines
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HCC1806, MDA-MB-436, HCC1395, ZR-75-1, T47D, MCF7, BT474, and MCF10A were a kind gift from CRUK Manchester Institute and they were authenticated by short tandem repeat profiling by CRUK MI core facility unit. HCC1806 was authenticated again in August 2017 by ATCC Service. HCC1569 and HCC1599 were purchased from ATCC in April 2016. MDA-MB-453 and CAL-85-1 were purchased from DSMZ in November 2014 and November 2016, respectively. MDA-MB-321 and BT20 was a kind gift from Department of Immunology, Medical University of Warsaw (purchased from ATCC in February 2018). ATCC and DSMZ authenticate cell lines by short tandem repeat profiling. HCC1806, HCC1395, BT474, BT20, ZR-75-1, T47D, HCC1599 and HCC1569 were cultured in RPMI-1640 supplemented with 10% FCS, 1% penicillin/streptomycin, 2 mM l -glutamine and 1 mM sodium pyruvate. MCF7, CAL-85-1, MDA-MB-453 and MDA-MB-231 were cultured in DMEM supplemented with 10% FCS, 1% penicillin/streptomycin, 2 mM l -glutamine and 1 mM sodium pyruvate. MDA-MB-436 were cultured in RPMI-1640 with 25 mM HEPES supplemented with 10% FCS, 1% penicillin/streptomycin, 2 mM l -glutamine, 1 mM sodium pyruvate and 10 µg/ml insulin. MCF10A were grown in DMEM/F12 supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 mg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 µg/ml insulin, 1% penicillin/streptomycin.
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