Tnf alpha quantikine elisa kit

Manufactured by R&D Systems

Sourced in United States

The TNF-alpha Quantikine ELISA Kit is a solid-phase enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of tumor necrosis factor-alpha (TNF-alpha) in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Il 6 quantikine elisa kit, by R&D Systems (2 mentions) Insulin elisa kit, by R&D Systems (1 mentions) Glucose colorimetric assay kit, by Cayman Chemical (1 mentions) Alanine transaminase colorimetric activity assay kit, by Cayman Chemical (1 mentions) Cholesterol fluorometric assay kit, by Cayman Chemical (1 mentions) Il 6 duoset elisa kit, by R&D Systems (1 mentions) Vevo 2100, by Fujifilm (1 mentions) Enzyme linked immunosorbent assay kit, by Chondrex (1 mentions) Quantikine elisa, by R&D Systems (1 mentions) Vegf quantikine elisa kit, by R&D Systems (1 mentions) Amicon ultra 0.5 centrifugal filter unit, by Merck Group (1 mentions) Il 1 beta il 1f2 quantikine elisa kit, by R&D Systems (1 mentions)

Spelling variants of this product

Quantikine ELISA kit (1163 citations) Quantikine ELISA (398 citations) ELISAs (144 citations) Quantikine kits (80 citations) Quantikines (4 citations)

6 protocols using tnf alpha quantikine elisa kit

Quantification of TNF-α and IFN-γ

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The levels of TNF-α and IFN-γ in blood serum and bone marrow fluids were measured with TNF-alpha Quantikine ELISA Kit (R&D Systems, MTA00B) and IFN-gamma Quantikine ELISA Kit (R&D Systems, MIF00), respectively, according to the manufacturer’s protocol.

Fujino T., Goyama S., Sugiura Y., Inoue D., Asada S., Yamasaki S., Matsumoto A., Yamaguchi K., Isobe Y., Tsuchiya A., Shikata S., Sato N., Morinaga H., Fukuyama T., Tanaka Y., Fukushima T., Takeda R., Yamamoto K., Honda H., Nishimura E.K., Furukawa Y., Shibata T., Abdel-Wahab O., Suematsu M, & Kitamura T. (2021). Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway. Nature Communications, 12, 1826.

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Biochemical Assays for Metabolic Markers

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All biochemical parameters were measured using an enzyme-linked immunosorbent assay kit (ELISA). An Alanine Transaminase Colorimetric Activity Assay Kit, Cholesterol Fluorometric Assay Kit, and Glucose Colorimetric Assay Kit were purchased from Cayman Chemical, a TNF-alpha Quantikine ELISA Kit and an Insulin ELISA Kit from R&D Systems, and a Rat Lipopolysaccharides (LPS) ELISA Kit from MyBioSource. Insulin resistance (HOMA-IR) was calculated according to the following formula: fasting serum glucose × fasting serum insulin/22.5.

Maciejewska D., Łukomska A., Dec K., Skonieczna-Żydecka K., Gutowska I., Skórka-Majewicz M., Styburski D., Misiakiewicz-Has K., Pilutin A., Palma J., Sieletycka K., Marlicz W, & Stachowska E. (2019). Diet-Induced Rat Model of Gradual Development of Non-Alcoholic Fatty Liver Disease (NAFLD) with Lipopolysaccharides (LPS) Secretion. Diagnostics, 9(4), 205.

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Quantification of Serum and Lung TNF-α and IL-6

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Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were quantified in serum samples and lung homogenates by specific rat TNF-alpha Quantikine ELISA Kit (RTA00, R&D System, USA) and IL-6 DuoSet ELISA Kit (DY506, R&D System, USA) according to the manufacturer’s instruction.

Morales-Cano D., Callejo M., Barreira B., Mondejar-Parreño G., Esquivel-Ruiz S., Ramos S., Martín M.Á., Cogolludo A., Moreno L, & Perez-Vizcaino F. (2019). Elevated pulmonary arterial pressure in Zucker diabetic fatty rats. PLoS ONE, 14(1), e0211281.

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Echocardiography and Cardiac Injury Modeling

Cited 3 times

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Echocardiography was performed with a VisualSonics Vevo 2100 imaging system as described previously.^{23 (link)} Transverse aortic constriction (TAC) and myocardial infarction (MI) were performed as previously described.^{24 (link),25 (link)} Mouse HMGB1 plasma levels were measured using an enzyme-linked immunosorbent assay kit from Chondrex.^{23 (link)} cTnI plasma levels were measured using an ELISA kit from Life Diagnostics, Inc. TNFα plasma levels were measured using a TNF-alpha Quantikine ELISA kit from R&D Systems.

Guo X., Yin H., Li L., Chen Y., Li J., Doan J., Steinmetz R, & Liu Q. (2017). Cardioprotective Role of TRAF2 by Suppressing Apoptosis and Necroptosis. Circulation, 136(8), 729-742.

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Quantification of Cytokines in Cell Media

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CM of A549 and H358 cells were obtained as described in Section 2.3. Subsequently, Quantikine^® ELISAs from R&D Systems were used for quantification of VEGF-A (VEGF Quantikine ELISA Kit, #DVE00), IL-6 (IL-6 Quantikine ELISA Kit, #D6050), IL-8 (IL-8/CXCL8 Quantikine ELISA Kit, #D8000C) and TNFα (TNF-alpha Quantikine ELISA Kit, #DTA00D). To detect TNFα, 2 mL of CM from a treatment group was pooled and concentrated using the Amicon^® Ultra-0.5 Centrifugal Filter Unit (Merck; NMWL: 3 kDa).

Wittig F., Pannenberg L., Schwarz R., Bekeschus S., Ramer R, & Hinz B. (2023). Antiangiogenic Action of JZL184 on Endothelial Cells via Inhibition of VEGF Expression in Hypoxic Lung Cancer Cells. Cells, 12(19), 2332.

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Quantification of Inflammatory Biomarkers

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Serum levels of IL-6, TNFα, IL-1β, and CRP were determined using IL-6 Quantikine ELISA Kit (M6000B, R&D, Minneapolis, MN, USA), TNF-alpha Quantikine ELISA Kit (MTA00B, R&D, Minneapolis, MN, USA), IL-1 beta/IL-1F2 Quantikine ELISA Kit (MLB00C, R&D, Minneapolis, MN, USA), C-Reactive Protein/CRP Quantikine ELISA Kit (MCRP00, R&D, Minneapolis, MN, USA), respectively.

Tang G., Du Y., Guan H., Jia J., Zhu N., Shi Y., Shu R., & Yuan W. (2020). Butyrate ameliorate skeletal muscle atrophy in Diabetic Nephropathy via enhancing gut barrier function and GPR43 mediated PI3K/AKT/mTOR signals.

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