The following antibodies were used in this study: anti-H3K4me1 (Millipore Sigma 07-436, Burlington, MA, 1:2000), anti-H3K4me2 (Millipore Sigma 07-030, 1:1000), anti-H3K4me3 (Millipore Sigma 07-473 or 04-745, 1:2000), anti-H3 (Abcam 1791, Cambridge, UK, 1:3000), rat monoclonal anti-Ser2P CTD (3E10, Dirk Eick, 1:1000), rat monoclonal anti-Ser5P CTD (3E8, Dirk Eick, 1:3000), mouse monoclonal anti-CTD for total Rpb1 (8WG16, Buratowski lab, 1:1000), anti-Set1 (Santa Cruz, sc-101858, 1:1000), anti-TBP polyclonal antiserum (Buratowski lab, 1:3000), anti-Myc (MMS-150R-500, Covance, 1:2000), anti-β-Actin (Abcam 8224, 1:2000), anti-HA (3F10, Roche and 12CA5, 1:2000), anti-Gal4 DBD (sc-577, Santa Cruz, 1:1000).
Anti set1
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom, Netherlands
Anti-Set1 is a laboratory reagent designed for use in epigenetic research. It is a monoclonal antibody that specifically binds to the SET1 protein, which is a histone methyltransferase involved in the regulation of gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Anti h3k4me3, by Merck Group (1 mentions)
Anti h3k4me1, by Merck Group (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti h3, by Abcam (1 mentions)
Anti myc, by Fortrea (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Nitrocellulose membrane, by Cytiva (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
Complete protease inhibitor, by Roche (1 mentions)
Whatman, by Cytiva (1 mentions)
2 protocols using anti set1
1
Chromatin Immunoprecipitation Antibody Panel
2
Yeast Protein Extraction and Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Yeast extracts for western blot analysis were prepared using standard glass bead disruption into a buffer A (50 mM Tris HCl at pH7.5, 2 M Sucrose, 5 mM MgCl2, 1mM DTT, Complete protease inhibitor cocktail from Roche, Basel, Switzerland), 45 min at 4 °C. Lysed cells were centrifuged at 3100 rpm for 15 min at 4 °C and pellets were resuspended in buffer B (20 mM HEPES pH 7.5, 1.5 mM MgCl2, 0.5 M NaCl, 0.2 mM EDTA, 20% Glycerol, 1% Triton X-100, 1 mM DTT, Complete protease inhibitor, Roche, Basel, Switzerland).
Yeast extracts were loaded on 15% (histones) or 10% SDS-PAGE polyacrylamide gels and after the run transferred on nitrocellulose membranes (Whatman, Little Chalfont, UK) by TransBlot method (Bio-Rad, Hercules, CA, USA) in 25 mM Tris, 192 mM Glycine, 29% Methanol, 1 h, 100 V at 4 °C.
Membranes were hybridized with the following antibodies: H3 (Active Motif, Carlsbad, CA, USA, rabbit polyclonal 1:1000); anti-tri-methyl H3K4me3 (Cell Signaling, rabbit polyclonal, 1:1000); anti-di-methyl H3K4me2 (Active Motif, rabbit monoclonal, 1:1000); TAP-tag Antibody (GeneScript, Rijswijk, The Netherlands, rabbit polyclonal, 1:1000); anti-Set1 (Santa Cruz Biotechnology, Dallas, TX, USA sc-101858, mouse monoclonal 1:1000). Chemiluminescence signals intensity ratios were quantified by chemiluminescence imaging with the ChemiDoc™ XRS (Bio-Rad).
Yeast extracts were loaded on 15% (histones) or 10% SDS-PAGE polyacrylamide gels and after the run transferred on nitrocellulose membranes (Whatman, Little Chalfont, UK) by TransBlot method (Bio-Rad, Hercules, CA, USA) in 25 mM Tris, 192 mM Glycine, 29% Methanol, 1 h, 100 V at 4 °C.
Membranes were hybridized with the following antibodies: H3 (Active Motif, Carlsbad, CA, USA, rabbit polyclonal 1:1000); anti-tri-methyl H3K4me3 (Cell Signaling, rabbit polyclonal, 1:1000); anti-di-methyl H3K4me2 (Active Motif, rabbit monoclonal, 1:1000); TAP-tag Antibody (GeneScript, Rijswijk, The Netherlands, rabbit polyclonal, 1:1000); anti-Set1 (Santa Cruz Biotechnology, Dallas, TX, USA sc-101858, mouse monoclonal 1:1000). Chemiluminescence signals intensity ratios were quantified by chemiluminescence imaging with the ChemiDoc™ XRS (Bio-Rad).
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