Unless stated otherwise, antibodies for phenotype analysis were purchased from BD Pharmingen (San Diego, CA, USA) and were the following: fluorescein isothiocyanate-conjugated HLA-DR, CD80, IgG2A, CD52 (Bio-Rad, Hercules, CA, USA) and IgG2B (Bio-Rad); phycoerythrin-conjugated CD1a, CD86, IgG1 and CD83 (Beckman Coulter, Brea, CA, USA); phycoerythrin-Cy7- conjugated CD14 (eBioscience, San Diego, CA, USA), ILT-3 (Beckman Coulter) and IgG1 (eBioscience); PercPCy5.5-conjugated CD209 and IgG2B; and allophycocyanin-conjugated IgG1, CD3, CD25, PD-L1 (eBioscience) and CD40 (eBioscience). DCs were incubated with a mix of monoclonal antibodies for 30 min on ice. Cells were washed in fluorescence-activated cell sorting (FACS) buffer containing 1% fetal bovine serum and 0.05% sodium azide (Sigma-Aldrich) and analyzed using FACSCanto or Fortessa (BD Biosciences). Data were analyzed using FACSDiva 8 (BD Biosciences) and FlowJo 10 software (Ashland, Oregon, USA).
Phycoerythrin cy7 conjugated cd14
Manufactured by Thermo Fisher Scientific
Sourced in United States
Phycoerythrin-Cy7-conjugated CD14 is a fluorescently-labeled antibody used for the detection and analysis of CD14-expressing cells in flow cytometry applications. The phycoerythrin-Cy7 fluorophore conjugated to the CD14 antibody allows for multicolor flow cytometric analysis.
Automatically generated - may contain errors
2 protocols using phycoerythrin cy7 conjugated cd14
1
Dendritic Cell Phenotype Analysis
2
Dendritic Cell Phenotype Analysis
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Unless stated otherwise, antibodies for phenotype analysis were purchased from BD Pharmingen (San Diego, CA, USA) and were the following: fluorescein isothiocyanate-conjugated HLA-DR, CD80, IgG2A, CD52 (Bio-Rad, Hercules, CA, USA) and IgG2B (Bio-Rad); phycoerythrin-conjugated CD1a, CD86, IgG1 and CD83 (Beckman Coulter, Brea, CA, USA); phycoerythrin-Cy7- conjugated CD14 (eBioscience, San Diego, CA, USA), ILT-3 (Beckman Coulter) and IgG1 (eBioscience); PercPCy5.5-conjugated CD209 and IgG2B; and allophycocyanin-conjugated IgG1, CD3, CD25, PD-L1 (eBioscience) and CD40 (eBioscience). DCs were incubated with a mix of monoclonal antibodies for 30 min on ice. Cells were washed in fluorescence-activated cell sorting (FACS) buffer containing 1% fetal bovine serum and 0.05% sodium azide (Sigma-Aldrich) and analyzed using FACSCanto or Fortessa (BD Biosciences). Data were analyzed using FACSDiva 8 (BD Biosciences) and FlowJo 10 software (Ashland, Oregon, USA).
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