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Andor solis software

Manufactured by Oxford Instruments
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Andor Solis software is a comprehensive data acquisition and analysis platform developed by Oxford Instruments. It provides a user-friendly interface for controlling a wide range of scientific instrumentation and acquiring high-quality data.

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Lab products found in correlation

Illuminator hxp 200c, by Zeiss (5 mentions) Axio examiner d1 microscope, by Zeiss (5 mentions) Andor ixon ultra, by Oxford Instruments (5 mentions) Epiplan neo uar 50x 0.55 dic, by Zeiss (3 mentions) Fl filter set 38 he gfp, by Zeiss (3 mentions) Fl filter set 38 he gfp shift free, by Zeiss (2 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Nis elements advanced research software, by Nikon (1 mentions) Ec epiplan neofluar 10x 0, by Zeiss (1 mentions) Cell b software, by Olympus (1 mentions) Ix71 fluorescence microscope, by Olympus (1 mentions)

Spelling variants of this product

Solis software (13 citations) Andor Solis image-analysis software (6 citations) Andor SOLIS (2 citations) Andor Solis (I) imaging software (2 citations)

10 protocols using andor solis software

1

High-Resolution Live-Cell Imaging

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Imaging was performed through a 50× objective (LD EC Epiplan-Neofluar 50×/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used for fluorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 s at 512 × 512 pixel resolution. The exposure time was 200 ms (5 Hz).
Afify A, & Potter C.J. (2020). Insect repellents mediate species-specific olfactory behaviours in mosquitoes. Malaria Journal, 19, 127.
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2

Calcium Imaging of Tadpole Lateral Line

Cited 1 time
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In immobilized tadpoles, the head skin was cut along the midline using superfine scissors to minimize the stretching and the damage to the peripheral LL organs. The hindbrain dorsal roof was opened and some ependymal cells inside the neurocoele were removed to expose neuronal somata using a tungsten dissection needle (47 (link)). Tadpoles were left further in 5 µM Fluo-4 AM (Thermo Fisher Scientific, UK) saline solution for ∼20 min in darkness. After resting the tadpole for ∼20 min, fluorescence images were captured at 10 Hz using a ×10 water immersion lens with a Neo5.5 CMOS camera and the Andor Solis software (Oxford Instruments, UK), around the time when the anterior lateral line was activated by suction. Regions of interest (ROIs) were chosen based on visible increases of fluorescence intensity following suction during video replay. A large blank area void of tissue was chosen to determine background illumination, which was subtracted from all ROI fluorescence intensity measurements. Fluorescence intensities were given as change of intensity in percentages compared with the baseline, i.e., in the absence of sensory stimulation or motor activity at the same ROIs. Any fluorescence increase lower than 5% within 1 s after suction stimulus was classified as lack of response.
Saccomanno V., Love H., Sylvester A, & Li W.C. (2021). The early development and physiology of Xenopus laevis tadpole lateral line system. Journal of Neurophysiology, 126(5), 1814-1830.
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3

Live-Cell Fluorescence Microscopy

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Imaging was performed through a 50x objective (LD EC Epiplan-Neo uar 50x/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP lter cube (FL Filter Set 38 HE GFP shift free) were used for uorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 seconds at 512x512 pixel resolution. The exposure time was 200 ms (5 Hz).
Afify A., & Potter C.J. (2020). Insect Repellents Mediate Species-Specific Olfactory Behaviors in Mosquitoes.
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4

Live-Cell Fluorescence Microscopy

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Imaging was performed through a 50x objective (LD EC Epiplan-Neo uar 50x/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP lter cube (FL Filter Set 38 HE GFP shift free) were used for uorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 seconds at 512x512 pixel resolution. The exposure time was 200 ms (5 Hz).
Afify A., & Potter C.J. (2020). Insect Repellents Mediate Species-Specific Olfactory Behaviours in Mosquitoes.
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5

Live-Cell Fluorescence Microscopy

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Imaging was performed through a 50x objective (LD EC Epiplan-Neo uar 50x/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP lter cube (FL Filter Set 38 HE GFP shift free) were used for uorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 seconds at 512x512 pixel resolution. The exposure time was 200 ms (5 Hz).
Afify A., & Potter C.J. (2020). Insect Repellents Mediate Species-Specific Olfactory Behaviors in Mosquitoes.
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6

Fluorescent Antennae Imaging Protocol

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Antennae were imaged through a 10x (Zeiss EC Epiplan-Neofluar 10x/0.25) or a 50x (LD EC Epiplan-Neofluar 50x/0.55 DIC) objective mounted on a Zeiss Axio Examiner D1 microscope. For fluorescence, a light source (Zeiss Illuminator HXP 200C) and eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used.
For image acquisition, an EMCCD camera (Andor iXon Ultra, Oxford Instruments), NIS Elements Advanced Research software (Nikon instruments), and Andor Solis software (Oxford Instruments) were used. Recordings were for 20 seconds, at a resolution of 512×512 pixels, and an exposure time of 200 ms (5 Hz).
Afify A., Betz J.F., Riabinina O., Lahondère C, & Potter C.J. (2019). Commonly used insect repellents hide human odors from Anopheles mosquitoes. Current biology : CB, 29(21), 3669-3680.e5.
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7

Raman Spectroscopy of Monolayer and Embedded Cells

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Monolayer cells were trypsinized and resuspended in phosphate buffered saline (PBS) prior to Raman spectroscopy, whereas embedded cells were immersed in PBS to prevent dehydration. For data acquisition, a custom-built Raman spectrometer was connected to an Olympus IX71 fluorescence microscope (Olympus, Hamburg, Germany) and equipped with an 85-mW, 785-nm diode laser (TOPTICA Photonics, Gräfelfing, Germany), a blocking filter to separate the elastic Rayleigh scattering, and a spectrograph (Kaiser Optical Systems, Ann Arbor, MI, USA) with an iDus CCD camera (Andor Technology, Belfast, UK). Thirty individual cells were analysed per experiment with 100 s integration time. Data were acquired using Andor Solis software (Andor Technology) and Cell^B software (Olympus). A background spectrum was taken from each focal plane for subsequent data analysis.
Ehlicke F., Köster N., Salzig D, & Czermak P. (2017). Non-invasive Raman Spectroscopy and Quantitative Real-Time PCR Distinguish Among Undifferentiated Human Mesenchymal Stem Cells and Redifferentiated Nucleus Pulposus Cells and Chondrocytes In Vitro. The Open Biomedical Engineering Journal, 11, 72-84.
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8

Optogenetic Stimulation: Blue Light Intensity

Cited 1 time
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In a typical experimental procedure, the field of view was chosen using the 20× objective. The protocol applied consisted in steps of increasing blue light intensity (corresponding to 10% of the LED power) equal to 7 mW/cm2 (± 1 mW/cm2) measured at the sample from 0 to 67 mW/cm2. Each recording consisted of 10 pulses of blue light (470 nm) applied to a defined area or to the whole field of view. Pulses duration chosen were 10, 100, 250, and 500 ms and each pulse was separated by an interval of 5.5 s. Recordings were performed under continuous green illumination (550 nm, 7% corresponding to 15 mW/cm2 measured at the sample) at 10 frames/s for a total duration of 1 min per field of view (600 frames). The protocols were externally triggered through the LabVIEW script designed in our laboratory and the image acquisition was simultaneously performed through the Andor Solis software (Andor Technology, Belfast, United Kingdom) (Usami et al., 2008 (link)).
Afshar Saber W., Gasparoli F.M., Dirks M.G., Gunn-Moore F.J, & Antkowiak M. (2018). All-Optical Assay to Study Biological Neural Networks. Frontiers in Neuroscience, 12, 451.
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9

IbpA Localization in E. coli

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To observe the IbpA localization in cells, we used the E. coli BW25113 wild-type strain carrying pCA24N-rhodanese and the dnaKJ deletion strain. Each strain carrying pBAD30-ibpA 5' UTR-ibpA-gfp was grown to an OD660 of ~0.4 at 37 °C in LB medium. Cells were observed with an inverted microscope IX71 (Olympus) and a mercury lamp with a GFP filter. Fluorescent images were recorded with an iXon DV897 electron multiplying CCD camera (Andor) and the Andor SOLIS software (Andor).
Miwa T., Chadani Y, & Taguchi H. (2021). Escherichia coli small heat shock protein IbpA is an aggregation-sensor that self-regulates its own expression at posttranscriptional levels. Molecular microbiology, 115(1).
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10

IbpA Localization in E. coli

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To observe the IbpA localization in cells, we used the E. coli BW25113 wild-type strain carrying pCA24N-rhodanese and the dnaKJ deletion strain. Each strain carrying pBAD30-ibpA 5' UTR-ibpA-gfp was grown to an OD660 of ~0.4 at 37 °C in LB medium. Cells were observed with an inverted microscope IX71 (Olympus) and a mercury lamp with a GFP filter. Fluorescent images were recorded with an iXon DV897 electron multiplying CCD camera (Andor) and the Andor SOLIS software (Andor).
Miwa T., Chadani Y., & Taguchi H. (2020). Escherichia coli small heat shock protein IbpA is an aggregation-sensor that self-regulates its own expression at a translational level.
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