Chlorhexidine digluconate chx

Chlorhexidine digluconate (CHX) was from Sigma-Aldrich. BD™ Bacto™ brain heart infusion, BD BBL dehydrated brain heart infusion agar, and Corning™ Clear polystyrene 96-well microplates, Corning™ 3370 were obtained from Fisher. AlamarBlue assay kit was obtained from Fisher.
Triethylene glycol dimethacrylate (TEGDMA), 2-hydroxyethylmethacrylate (HEMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) were used as received (Sigma-Aldrich) without further purification. Camphorquinone (CQ), ethyl-4-(dimethylamino) benzoate (EDMAB) and diphenyliodonium hexafluorophosphate (DPIHP) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used as a three-component-photoinitiator system without further purification. ε-pL hydrochloride was purchased from Bonding Chemical (Katy, TX, USA). To obtain ε-pL), 10 g ε-pL hydrochloride was dissolved in 60 mL sodium bicarbonate buffer (NaHCO3, 1.5M) and dialyzed with membrane (Spectra/Por®7 Dialysis Tubing, MWCO 1kD) in deionized water for 4 days. Then the solution was dried at 37 °C and ε-pL was obtained.

Twelve commercial essential oils (Essential Oils Direct Ltd., Oldham, UK) (Table 5), two terpenes (E-cinnamaldehyde and linalool (Sigma-Aldrich, Gillingham, UK)), chlorhexidine digluconate (CHX) (Sigma-Aldrich, Gillingham, UK) and triclosan (Irgasan from Sigma-Aldrich, Gillingham, UK) were evaluated.
The commercial essential oils were tested at a range of concentrations against planktonic growth (2% (v/v) to 0.007% (v/v) and biofilms (8% (v/v) to 0.125% (v/v)). All agents were prepared in Sabouraud Dextrose Broth (SDB; Oxoid Ltd, Basingstoke, UK). To enhance dispersion of essential oils in the medium, 1% (v/v) Tween 80 (Sigma-Aldrich, Gillingham, UK) was added. In the case of biofilm studies, 0.015% (w/v) Agar Bacteriological (LP0011 Oxoid) was added to SDB [53 (link)]. CHX was used in SDB at concentrations between 0.04% (v/v) to 3.1 × 10⁻⁴% (v/v) and from 0.08% (v/v) to 6.2 × 10⁻⁴% (v/v) for planktonic and biofilm growth experiments, respectively. A 20% (w/v) stock solution of triclosan was prepared in Dimethyl Sulfoxide (DMSO) (Fisher Chemical, Loughborough, UK). Serial doubling dilutions of the stock solution were prepared in SDB yielding final concentrations from 5.2 × 10⁻⁶% (v/v) to 6.7 × 10⁻⁴% (v/v) and from 1.7 × 10⁻⁴% (v/v) to 5 × 10⁻³ (v/v) for planktonic and biofilm experiments, respectively.