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Hk210 01

Manufactured by Hycult Biotech
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Sourced in Netherlands

The HK210-01 is a laboratory instrument designed for analytical purposes. It is a high-performance product developed by Hycult Biotech to support various scientific applications. The core function of this equipment is to perform analysis and measurement tasks in a controlled laboratory environment.

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Lab products found in correlation

Cytation 1, by Agilent Technologies (2 mentions) Cell death elisaplus, by Roche (2 mentions) Odyssey clx, by LI COR (1 mentions) Image studio software, by LI COR (1 mentions) Donkey anti mouse 680rd, by LI COR (1 mentions) Prmt5, by Abcam (1 mentions) Sym10, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions)

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HK210 (3 citations)

4 protocols using hk210 01

1

Quantifying Myeloperoxidase Activity

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The tissue isolation and the myeloperoxidase assay were performed as described (24 ). Briefly, diluted sample MPO was bound to anti-MPO coated plates (Hycult Biotech HK210–01) followed by washes. Fluorescence (ex 535nm, em 590nm) after addition of H2O2 and ADHP was acquired in kinetic mode (10min, every 30 sec) on a Biotek Cytation 1. Results reported as RFU/second.
Lewis B.W., Amici S.A., Kim H.Y., Shalosky E.M., Khan A.Q., Walum J., Gowdy K.M., Englert J.A., Porter N.A., Grayson M.H., Britt RD J.r, & Guerau-de-Arellano M. (2022). PRMT5 in T cells drives Th17 responses, mixed granulocytic inflammation and severe allergic airway inflammation. Journal of immunology (Baltimore, Md. : 1950), 208(7), 1525-1533.
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2

Quantification of NETs in Mouse Serum

Cited 1 time
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To quantify NETs in mouse serum, a capture enzyme-linked immunosorbent assay for myeloperoxidase (MPO) associated with DNA was performed as described (18 (link), 32 (link)). For the capture antibody, a mouse MPO enzyme-linked immunosorbent assay kit (Hycult Biotech; HK210-01) was used according to the manufacturer's directions. A peroxidase-labeled anti-DNA monoclonal antibody (component 2, Cell Death ELISA PLUS, catalog no. 11774424001; Roche) was used. Serum nucleosome quantification was performed using the Cell Death kit.
Zhang H., Goswami J., Varley P., van der Windt D.J., Ren J., Loughran P., Yazdani H., Neal M.D., Simmons R.L., Zhang J., Tsung A, & Huang H. (2020). Hepatic Surgical Stress Promotes Systemic Immunothrombosis That Results in Distant Organ Injury. Frontiers in Immunology, 11, 987.
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3

Quantifying Neutrophil Extracellular Traps in Tumors

Cited 2 times
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To quantify NETs in mouse tumor homogenate, a capture enzyme-linked immunosorbent assay for myeloperoxidase (MPO) associated with DNA was performed as described [25 (link),26 (link)]. For the capture antibody, a mouse MPO enzyme-linked immunosorbent assay kit (HK210-01; Hycult Biotech, Uden, Netherlands) was used according to the manufacturer’s directions. A peroxidase-labeled anti-DNA monoclonal antibody (component 2, Cell Death ELISA PLUS, Roche, Basel, Switzerland) was used. Serum nucleosome quantification was performed using the Cell Death kit [24 (link)].
Zhang H., Wang Y., Onuma A., He J., Wang H., Xia Y., Lal R., Cheng X., Kasumova G., Hu Z., Deng M., Beane J.D., Kim A.C., Huang H, & Tsung A. (2021). Neutrophils Extracellular Traps Inhibition Improves PD-1 Blockade Immunotherapy in Colorectal Cancer. Cancers, 13(21), 5333.
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4

Lung Tissue Myeloperoxidase and Protein Analysis

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The tissue isolation and the myeloperoxidase assay were performed as described (20) . Briefly, diluted sample MPO was bound to anti-MPO coated plates (Hycult Biotech HK210-01) followed by washes. Fluorescence (ex 535nm, em 590nm) after addition of H2O2 and ADHP was acquired in kinetic mode (10min, every 30 sec) on a Biotek Cytation 1. Results reported as RFU/second. Western blotting. Right lung lobes were homogenized and lysed in RIPA buffer (10 mM Tris pH 8.0, 5 M NaCl, 0.5 M EDTA, 1% Triton X-100, 0.1% SDS, 0.1% sodium deoxycholate) containing protease and phosphatase inhibitors (ThermoFisher Scientific). Primary antibodies: PRMT5 (Abcam ab31751, 1:1000), H4R3 (MilliporeSigma SAB4300870, 1:500), SYM10 (MilliporeSigma 07-412, 1:300), ROR-gt (Life Technologies 14-6981-82, 1µg/ml) and β-actin (Sigma-Aldrich A1978, 1:50,000). Secondary antibodies (1:20,000): donkey anti-rabbit or anti-rat 800CW and donkey-anti-mouse 680RD (Li-cor). Blots were imaged on an Odyssey-CLx and quantified with Image Studio software (Li-Cor).
Lewis B.W., Amici S.A., Kim H., Shalosky E.M., Khan A., Walum J., Gowdy K.M., Englert J.A., Porter N.A., Grayson M.H., Britt R.D., & Guerau‐de‐Arellano M. (2021). PRMT5 in T cells drives Th17 responses, mixed granulocytic inflammation and severe allergic airway inflammation.
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