Bioapex

¹H NMR (400 MHz), ¹³C NMR (100 MHz), DEPT-135 and 2D NMR spectra were registered on a Varian AS 400 (Varian Inc., Palo Alto, CA, USA) using the residual solvent signal as an internal standard. High-resolution mass spectra were recorded using a Bruker BioApex (Bruker Corporation) machine. Pre-coated silica gel G-25 UV254 plates were used for thin layer chromatography (TLC) (20 cm × 20 cm) (E. Merck, Darmstadt, Germany). Silica gel (Purasil 60A, 230–400 mesh) was used for flash column chromatography (Whatman, Sanford, ME, USA).

¹H NMR (400 MHz) and ¹³C NMR (100 MHz) spectra were recorded using the residual solvent signal as an internal standard on a Varian AS 400 (Varian Inc., Palo Alto, CA, USA). High-resolution mass spectra were recorded using a Bruker BioApex (Bruker Corporation). Pre-coated silica gel G-25 UV254 plates were used for thin-layer chromatography (TLC) (20 cm × 20 cm) (E. Merck, Darmstadt, Germany). Silica gel (Purasil 60Å, 230–400 mesh) was used for flash column chromatography (Whatman, Sanford, ME, USA). Semi-preparative high-performance liquid chromatography (HPLC) was performed on a Cosmosil 5 C18–MS–II (150 × 4.6 mm) at a flow rate of 0.5 mL/min. The column was equipped with a TOSOH RI-8020 detector and a JASCO BIP-I HPLC pump [20 (link)].