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Cd45 microbead kit

Manufactured by Miltenyi Biotec
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Sourced in Germany

The CD45 Microbead kit is a laboratory equipment product designed for the isolation and enrichment of CD45-positive cells from various biological samples. The core function of this kit is to enable the efficient separation and purification of leukocytes or other cell types expressing the CD45 antigen.

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Lab products found in correlation

Adult brain dissociation kit, by Miltenyi Biotec (2 mentions) Hcd19, by Thermo Fisher Scientific (1 mentions) Hcd45, by BioLegend (1 mentions) Hcd33, by Beckman Coulter (1 mentions) Hcd34, by BD (1 mentions)

Spelling variants of this product

CD45 MicroBeads (177 citations) MicroBead kits (12 citations) CD45 MicroBeads kit (4 citations) REAlease CD45 (TIL) MicroBead Kit (2 citations) CD45 (TIL) MicroBead kit (2 citations)

3 protocols using cd45 microbead kit

1

Immune Cell Isolation and Co-culture with OPCs

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CFA and EAE mice were perfused in PBS and the spinal cords were collected. Immune cells (lymphocytes, monocytes and macrophages) were then isolated using the adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) according to the manufacturer’s protocol and collected with MACS with CD45+ microbeads (CD45 Microbead kit, Miltenyi Biotec 130-052-301) according to the manufacturer’s protocol. CD45+ cells (150-200,000) were added on top of cultured OPCs (300,000 cells) and incubated for 72hours.
Falcão A.M., van Bruggen D., Marques S., Meijer M., Jäkel S., Agirre E., S.a.m.u.d.y.a.t.a., Floriddia E.M., Vanichkina D.P., ffrench-Constant C., Williams A., Guerreiro-Cacais A.O, & Castelo-Branco G. (2018). Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis. Nature medicine, 24(12), 1837-1844.
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2

Engraftment and Lineage Analysis of Human Hematopoietic Cells in NSG Mice

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NSG mice were irradiated with 1.5 Gy 24 h prior transplantation. Approximately 105 CD34+ cells were transplanted into NSG mice by retro-orbital injection. Once a month, bone marrow aspirations were performed to analyze in BM cells the engraftment and lineage distribution by FACS, using hCD45 (#304014, BioLegend), hCD19 (#25-0198, eBioscience), hCD33 (#A07775, Beckman Coulter), hCD34 (#555824, Becton Dickinson), and CD18 (#555923 or #551060, BD Pharmingen) monoclonal antibodies. Transplanted mice were culled at 3 months post-transplantation (mpt) and total bone marrow cells (BMCs) were also analyzed by FACS with the same antibodies. Human CD45+ magnetic-labeled cells were selected using CD45 MicroBead Kit (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany) and secondary transplants in NSG mice were performed with comparable number of cells in all groups (3–5 × 106 cells per mouse). After 3 months follow-up, secondary recipients were sacrificed by cervical dislocation and BM and PB samples were analyzed by flow cytometry.
Mesa-Núñez C., Leon-Rico D., Aldea M., Damián C., Sanchez-Baltasar R., Sanchez R., Alberquilla O., Segovia J.C., Bueren J.A, & Almarza E. (2020). The downregulated membrane expression of CD18 in CD34+ cells defines a primitive population of human hematopoietic stem cells. Stem Cell Research & Therapy, 11, 164.
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3

Immune Cell Isolation and Co-culture with OPCs

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CFA and EAE mice were perfused in PBS and the spinal cords were collected. Immune cells (lymphocytes, monocytes and macrophages) were then isolated using the adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) according to the manufacturer’s protocol and collected with MACS with CD45+ microbeads (CD45 Microbead kit, Miltenyi Biotec 130-052-301) according to the manufacturer’s protocol. CD45+ cells (150-200,000) were added on top of cultured OPCs (300,000 cells) and incubated for 72hours.
Falcão A.M., van Bruggen D., Marques S., Meijer M., Jäkel S., Agirre E., S.a.m.u.d.y.a.t.a., Floriddia E.M., Vanichkina D.P., ffrench-Constant C., Williams A., Guerreiro-Cacais A.O, & Castelo-Branco G. (2018). Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis. Nature medicine, 24(12), 1837-1844.
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