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Glutamyl γ hydroxamate

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Glutamyl-γ-hydroxamate is a chemical compound used in laboratory settings. It functions as a substrate for specific enzymatic reactions. The compound's core purpose is to facilitate the study and analysis of these enzymatic processes in a controlled experimental environment.

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Lab products found in correlation

Micro bio spin gel p 6 columns, by Bio-Rad (2 mentions)

2 protocols using glutamyl γ hydroxamate

1

Measuring Glutamyltransferase Activity

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Glul activity was assayed using the γ-glutamyltransferase assay as previously described67 (link). Briefly, WT and lf:Yap transgenic larvae or adult livers were lysed in 50 mM imidazole and samples were purified using micro bio-spin gel p-6 columns (BioRad). Lysates were incubated in activity buffer (50 mM imidazole, 50 mM L-glutamine, 25 mM hydroxylamine, 25 mM sodium arsenate, 2mM manganese chloride, 0.16 mM adenosine diphosphate) at 27 °C for 30 min. The reactions were quenched in stop solution (2.42% iron chloride and 1.45% trichloroacetic acid in 1.82 N hydrochloric acid). Absorbance was read at 520 nm and a calibration curve of glutamyl-γ-hydroxamate (Sigma) was used to calculate nmol of product formed.
Cox A.G., Hwang K.L., Brown K.K., Evason K., Beltz S., Tsomides A., O'Connor K., Galli G.G., Yimlamai D., Chhangawala S., Yuan M., Lien E.C., Wucherpfennig J., Nissim S., Minami A., Cohen D.E., Camargo F.D., Asara J.M., Houvras Y., Stainier D.Y, & Goessling W. (2016). Yap reprograms glutamine metabolism to increase nucleotide biosynthesis and enable liver growth. Nature cell biology, 18(8), 886-896.
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2

Measuring Glutamyltransferase Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glul activity was assayed using the γ-glutamyltransferase assay as previously described67 (link). Briefly, WT and lf:Yap transgenic larvae or adult livers were lysed in 50 mM imidazole and samples were purified using micro bio-spin gel p-6 columns (BioRad). Lysates were incubated in activity buffer (50 mM imidazole, 50 mM L-glutamine, 25 mM hydroxylamine, 25 mM sodium arsenate, 2mM manganese chloride, 0.16 mM adenosine diphosphate) at 27 °C for 30 min. The reactions were quenched in stop solution (2.42% iron chloride and 1.45% trichloroacetic acid in 1.82 N hydrochloric acid). Absorbance was read at 520 nm and a calibration curve of glutamyl-γ-hydroxamate (Sigma) was used to calculate nmol of product formed.
Cox A.G., Hwang K.L., Brown K.K., Evason K., Beltz S., Tsomides A., O'Connor K., Galli G.G., Yimlamai D., Chhangawala S., Yuan M., Lien E.C., Wucherpfennig J., Nissim S., Minami A., Cohen D.E., Camargo F.D., Asara J.M., Houvras Y., Stainier D.Y, & Goessling W. (2016). Yap reprograms glutamine metabolism to increase nucleotide biosynthesis and enable liver growth. Nature cell biology, 18(8), 886-896.
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