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P tazs89

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P-TAZS89 is a primary antibody that recognizes phosphorylated TAZ (transcriptional co-activator with PDZ-binding motif) at serine 89. It is used for the detection and analysis of TAZ phosphorylation status in biological samples.

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Lab products found in correlation

Pyaps127, by Cell Signaling Technology (2 mentions) Lats1, by Cell Signaling Technology (2 mentions) P lats1t1079, by Cell Signaling Technology (1 mentions) Hrp conjugated goat anti rabbit antibody, by Cell Signaling Technology (1 mentions) Hrp conjugated goat anti mouse antibody, by Cell Signaling Technology (1 mentions) Pan tead, by Cell Signaling Technology (1 mentions) Birc5, by Cell Signaling Technology (1 mentions) Pyap1 s127, by Cell Signaling Technology (1 mentions) Mob1a, by Cell Signaling Technology (1 mentions) Taz v386, by Cell Signaling Technology (1 mentions) Multi gauge software, by Fujifilm (1 mentions) Sc 101199, by Santa Cruz Biotechnology (1 mentions) β actin, by Merck Group (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions)

3 protocols using p tazs89

1

Immunoblotting and Immunohistochemistry Antibodies

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Antibodies for immunohistochemistry: NONO (sc‐166702, Santa Cruz Biotechnology) was used at a 1/200 dilution and secondary antibodies at a 1/200 dilution. Antibodies for immunoblotting: µ‐Actin (#3700, dilution at 1/20000), TAZ (#4883S, dilution at 1/1000), Pan‐Tead (#13 295, dilution at 1/2000), Rpb1 (#2629, dilution at 1/3000), p‐YAPS127 (#13 008, dilution at 1/1000), YAP (#12 395, dilution at 1/2000), p‐TAZS89 (#59 971, dilution at 1/1000), Myc (#2272, dilution at 1/3000), p‐Lats1T1079 (#8654, dilution at 1/500), and Lats1 (#3477, dilution at 1/1000), all from Cell Signaling Technologies; HA (MMS‐101P, Biolegend, dilution at 1/3000); and goat anti‐rabbit HRP‐conjugated antibody (#7074S, dilution at 1/5000) and goat anti‐mouse HRP‐conjugated antibody, (#7076S, dilution at 1/5000) both from Cell Signaling Technologies.
Wei Y., Luo H., Yee P.P., Zhang L., Liu Z., Zheng H., Zhang L., Anderson B., Tang M., Huang S, & Li W. (2021). Paraspeckle Protein NONO Promotes TAZ Phase Separation in the Nucleus to Drive the Oncogenic Transcriptional Program. Advanced Science, 8(24), 2102653.
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2

Immunoblotting Analysis of Hippo Pathway

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Immunoblotting was carried out using a standard protocol and primary antibodies recognizing MOB1A (#E1N9D; Cell Signaling Technology), MST1 (#3682S; Cell Signaling Technology), LATS1 (C66B5; Cell Signaling Technology), YAP1 (#4912S; Cell Signaling Technology), pYAP1(S127) (#4911S; Cell Signaling Technology), TAZ (V386; Cell Signaling Technology), pTAZ (S89) (#75275; Cell Signaling Technology), CTGF (L-20; Santa Cruz Biotechnology), BIRC5 (71G4B7; Cell Signaling Technology), TOP2A (EP1102Y; Abcam), or TP63 (4A4; Abcam). Primary antibodies were detected using horseradish peroxidase (HRP)–conjugated secondary rabbit antibody (#7074; Cell Signaling Technology). Endogenous GAPDH (FL-355; Santa Cruz Biotechnology) was used as the internal control. Quantification of signal intensity was performed using Fujifilm Multi Gauge software.
Omori H., Nishio M., Masuda M., Miyachi Y., Ueda F., Nakano T., Sato K., Mimori K., Taguchi K., Hikasa H., Nishina H., Tashiro H., Kiyono T., Mak T.W., Nakao K., Nakagawa T., Maehama T, & Suzuki A. (2020). YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma. Science Advances, 6(12), eaay3324.
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3

Immunoblotting Analysis of YAP and TAZ Signaling

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Neutrophils were lysed with 20 mmol/L Tris·HCl, pH 8.0, 100 mmol/L NaCl, 0.5% Triton X-100, 1 mmol/L EDTA, and 1 % protease inhibitor cocktail tablets (Roche) on ice for 30 min. Cell lysates were then boiled with 5× SDS loading buffer. The proteins were separated using SDS-PAGE, transferred to a PVDF membrane (Bio-Rad) and further incubated with antibodies. The antibodies used for immunoblotting targeted YAP (sc-101199, Santa Cruz, 1:500), pYAPS127 (13008, Cell Signaling Technology, 1:500), TAZ (22439, Abcam, 1:500), pTAZS89 (59971S, Cell Signaling Technology, 1:500), β-actin (A2228, Sigma-Aldrich, 1:5,000). Raw images and corresponding quantifications are shown in Table S6.
Nie P., Zhang W., Meng Y., Lin M., Guo F., Zhang H., Tong Z., Wang M., Chen F., An L., Tang Y., Han Y., Yu R., Wang W., Xu Y., Wei L., Zhou Z, & Jiao S. (2022). A YAP/TAZ-CD54 axis is required for CXCR2−CD44− tumor-specific neutrophils to suppress gastric cancer. Protein & Cell, 14(7), 515-533.
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