4 hydroxybenzenesulfonamide

NBBS was obtained from Ivy Fine Chemicals (CAS RN 3622–84-2; Lot IF10505; Cherry Hill, NJ). Identity of NBBS was confirmed by ¹H and ¹³C NMR and mass spectrometry (MS) and the purity (>99.9%) was determined by gas chromatography-with flame ionization detection. [¹⁴C]-labeled NBBS ([¹⁴C]NBBS) (uniformly ring labelled, 12.1 mCi, 0.19 mCi/mg in ethanol) was obtained from MRIGlobal (Kansas City, MO) and was stored at −20 °C. The radiochemical purity was determined to be >96% by high performance liquid chromatography (HPLC) methods 1 or 2 given below. A radiochemical purity of >94% was maintained throughout the studies by purifying the material using a 3-cc Oasis® HLB solid phase extraction cartridge (Waters, Milford, MA).
Ultima Gold™ liquid scintillation cocktail and Soluene®−350 were purchased from PerkinElmer Life and Analytical Sciences (Waltham, MA). Potential NBBS metabolites, benzenesulfonamide and 4-hydroxybenzenesulfonamide, β-Glucuronidase (from E. coli), sulfatase (from Aerobacter aerogenes), acylase (Type I, porcine kidney) were from Sigma-Aldrich (St. Louis, MO). Rapid Equilibrium Dialysis (RED) devices were from Thermo Scientific (Waltham, MA). All other reagents were obtained from commercial sources.

NBBS was obtained from Ivy Fine Chemicals (CAS RN 3622-84-2; Lot IF10505; Cherry Hill, NJ). Identity of NBBS was confirmed by ¹H and ¹³C NMR and mass spectrometry (MS) and the purity (>99.9%) was determined by gas chromatography-with flame ionization detection. [¹⁴C]-labeled NBBS ([¹⁴C]NBBS) (uniformly ring labelled, 12.1 mCi, 0.19 mCi/mg in ethanol) was obtained from MRIGlobal (Kansas City, MO) and was stored at −20 °C. The radiochemical purity was determined to be >96% by high performance liquid chromatography (HPLC) methods 1 or 2 given below. A radiochemical purity of >94% was maintained throughout the studies by purifying the material using a 3-cc Oasis® HLB solid phase extraction cartridge (Waters, Milford, MA).
Ultima Gold™ liquid scintillation cocktail and Soluene®-350 were purchased from PerkinElmer Life and Analytical Sciences (Waltham, MA). Potential NBBS metabolites, benzenesulfonamide and 4-hydroxybenzenesulfonamide, β-Glucuronidase (from E. coli), sulfatase (from Aerobacter aerogenes), acylase (Type I, porcine kidney) were from Sigma-Aldrich (St. Louis, MO). Rapid Equilibrium Dialysis (RED) devices were from Thermo Scientific (Waltham, MA). All other reagents were obtained from commercial sources.