Amicon ultra 10k 15 ml centrifugal filter

Example 10

IRDye700DX NHS Ester (LI-COR Bioscience, Inc.) was used to label P10-2 Fab with IRDye700DX.

Specifically, a P10-2 Fab solution adjusted to 1 mg/mL with phosphate-buffered saline (pH 7.4) was supplemented with a 1/10 amount of a 1 M dipotassium hydrogen phosphate solution (pH 9). IRDye700DX NHS Ester was added thereto at a final concentration of 154 μg/mL, and the resultant was stirred at room temperature for 2 hours. After reaction, IRDye700DX-labeled P10-2 Fab was purified by recovery through Amicon Ultra 10K-15 mL centrifugal filter (Merck Millipore). This IRDye700DX-labeled P10-2 Fab was designated as P10-2 Fab IR700.

Example 10

IRDye700DX NHS Ester (LI-COR Bioscience, Inc.) was used to label P10-2 Fab with IRDye700DX.

Specifically, a P10-2 Fab solution adjusted to 1 mg/mL with phosphate-buffered saline (pH 7.4) was supplemented with a 1/10 amount of a 1 M dipotassium hydrogen phosphate solution (pH 9). IRDye700DX NHS Ester was added thereto at a final concentration of 154 μg/mL, and the resultant was stirred at room temperature for 2 hours. After reaction, IRDye700DX-labeled P10-2 Fab was purified by recovery through Amicon Ultra 10K-15 mL centrifugal filter (Merck Millipore). This IRDye700DX-labeled P10-2 Fab was designated as P10-2 Fab IR700.

Example 10

IRDye700DX NHS Ester (LI-COR Bioscience, Inc.) was used to label P10-2 Fabwith IRDye700DX.

Specifically, a P10-2 Fab solution adjusted to 1 mg/mL with phosphate-buffered saline (pH 7.4) was supplemented with a 1/10 amount of a 1 M dipotassium hydrogen phosphate solution (pH 9). IRDye700DX NHS Ester was added thereto at a final concentration of 154 μg/mL, and the resultant was stirred at room temperature for 2 hours. After reaction, IRDye700DX-labeled P10-2 Fab was purified by recovery through Amicon Ultra 10K-15 mL centrifugal filter (Merck Millipore). This IRDye700DX-labeled P10-2 Fab was designated as P10-2 Fab IR700.