MCF-7 cells (6000 cells/well) were plated in 96-well culture plates with EMEM media supplemented with 10% CD (charcoal stripped)-FBS and allowed to adhere overnight. The following day, media was removed and replaced with EMEM + 10% CD-FBS + 1 nM 17-β-estradiol (MedChemExpress: HY-B0141) followed by treatment with the compound (final DMSO concentration in wells: 1.1%). Fulvestrant (MedChemExpress: HY-13636) and 4-hydroxytamoxifen (OHT, MedChemExpress: HY-16950) were used. After 24 h incubation, the media was removed and replaced with fresh media. Alamar blue solution (4 mg resazurin per 40 mL of PBS) was then added and cells were incubated for 2–6 h. Each of the well’s fluorescence (λexcit = 555 nm, λemission = 585 nm) was measured using a SpectraMax M3 plate reader (Molecular Devices). Percent dead was determined by comparison to a 100% dead control: 100 μM raptinal-treated cells. Dose-dependent curves and IC50 were calculated using Origin Pro V10.
17 β estradiol
Manufactured by MedChemExpress
Sourced in United States, China
17-β-estradiol is a synthetic steroid compound. It is a primary female sex hormone that plays a crucial role in the regulation of the menstrual cycle and the development of female secondary sexual characteristics.
Automatically generated - may contain errors
Lab products found in correlation
Spectramax m3, by Molecular Devices (1 mentions)
Acetylcholine, by Merck Group (1 mentions)
Phtpp, by MedChemExpress (1 mentions)
2 apb, by MedChemExpress (1 mentions)
U73343, by MedChemExpress (1 mentions)
Ska 31, by MedChemExpress (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by MedChemExpress (1 mentions)
Ouabain, by MedChemExpress (1 mentions)
Nω nitro l arginine, by Merck Group (1 mentions)
U73122, by MedChemExpress (1 mentions)
Tram 34, by MedChemExpress (1 mentions)
Ab150113, by Abcam (1 mentions)
Goat anti mouse igg h l alexa fluor 488, by Abcam (1 mentions)
Ab3576, by Abcam (1 mentions)
Tamoxifen, by MedChemExpress (1 mentions)
Ab33186, by Abcam (1 mentions)
Ab33333, by Abcam (1 mentions)
Ab181602, by Abcam (1 mentions)
Hrp conjugated affinipure goat anti mouse igg h l, by Proteintech (1 mentions)
4 protocols using 17 β estradiol
1
Assessing Anti-Proliferative Effects in MCF-7 Cells
2
Comprehensive Evaluation of Pharmacological Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All salts were from Sangon Biotech in Shanghai. Acetylcholine, Nω-nitro-L-arginine and indomethacin were offered by Sigma-Aldrich (St Louis, USA). NE was purchased from GRANDPHARMA (China) co. LTD. 17β-Estradiol, LPS, Propyl pyrazole triol (PPT), MPP hydrochloride (MPP), G-1, G-15, Diarylpropionitrile (DPN), PHTPP, ouabain, TRAM-34, apamin, U73122, U73343, 2-APB and SKA-31 were supplied by MedChemExpress (MCE; New Jersey, USA), SN-6 was provided by Tocris (Bristol, UK). LiCl was supplied by Macklin (China). The human phospholipase C Beta 1 Polyclonal antibody (Cat No. 26551-1-AP) and human ITPR1-specific Polyclonal antibody (Cat No. 19962-1-AP) were from proteintech. The commercial kits of serum ALT, AST, LD and BUN were from Neobioscience (Jiancheng Bioengineering Institute, Nanjing, China). The commercial kit of serum DAO was from Solarbio. The commercial kit of serum Cr was from Beijing Leagene Biotechnology co. LTD.
3
Regulation of Rac1, mTOR, and Cortactin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary antibodies were against Rac1 (ab33186, Abcam), GAPDH (ab181602, Abcam), phosphorylated (p)-mTOR (5536T, Cell Signaling Technology, United States), mTOR (2983T, Cell Signaling Technology), ERβ (ab3576, Abcam), and cortactin (ab33333, Abcam). The secondary antibodies were horseradish peroxidase (HRP)-linked anti-rabbit immunoglobulin G (IgG) (7074S, Cell Signaling Technology), HRP-conjugated AffiniPure goat anti-mouse IgG (H++L) (SA00001-1, Proteintech, United States), and goat anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113, Abcam). 17-β-Estradiol and tamoxifen was purchased from MedChemExpress to activate or modulate ER, respectively.
4
Rat Model of Prolactinoma and CAB Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 24 female rats were randomly divided into three groups: (i) sham (n = 8), (ii) control (Ctrl) (prolactinoma, n = 8), and (iii) CAB (prolactinoma + CAB, n = 8). To establish the in situ rat prolactinoma model, 1 cm silastic capsules containing 10 mg of 17-β estradiol (MedChemExpress, Shanghai, China, cat. E2758) were implanted into 4-week-old rats subcutaneously, as described in our previous reports [12 (link),13 (link)]. Five weeks later, an MRI was performed to validate the establishment of prolactinoma. At this point, the CAB (0.5 mg/kg, MedChemExpress, Shanghai, China) or control (Ctrl) 0.9%, saline (100 μL) was injected twice a week by gavage. Four weeks later, an MRI examination was conducted to measure tumor size, and the BP was subsequently measured.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!