Anti h2kk pe

Manufactured by BD

Sourced in United Kingdom

Anti-H2Kk-PE is a fluorescent-labeled antibody that binds to the H-2Kk major histocompatibility complex (MHC) class I molecule. It can be used to identify and quantify cells expressing the H-2Kk antigen through flow cytometry or other fluorescence-based applications.

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Lab products found in correlation

Anti cd86 pe, by Thermo Fisher Scientific (1 mentions) Anti cd11c apc, by BD (1 mentions) Anti cd80 pe, by BD (1 mentions) Anti cd40 fitc, by BD (1 mentions) Anti mhc class 2 pe, by Thermo Fisher Scientific (1 mentions) Anti cd54 pe, by BD (1 mentions)

2 protocols using anti h2kk pe

Bone Marrow Transplantation Protocol

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BMT was performed as previously described [26 (link)]. Briefly, recipient B10.BR (Allo) and C57BL/6J (Syn) mice were lethally irradiated using a Cesium irradiator (8 and 10.25 Gy respectively) and injected with 4×10⁶ bone marrow cells and 1×10⁶ splenocytes from donor mice (C57BL/6J). All mice used were >95% engrafted 4 weeks post BMT per engraftment analysis Engraftment of CD3⁺, CD19⁺, CD11b⁺, and CD11c⁺ (eBioscience, San Diego, CA) peripheral blood cells was evaluated 4 weeks post-transplantation using anti-H2D^b-FITC and anti-H2K^k-PE (BD Biosciences).

Gowdy K.M., Martinu T., Nugent J.L., Manzo N.D., Zhang H., Kelly F.L., Holtzman M.J, & Palmer S.M. (2014). Impaired CD8+ T cell immunity after allogeneic bone marrow transplantation leads to persistent and severe respiratory viral infection. Transplant immunology, 32(1), 51-60.

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Multiparametric Flow Cytometry of Dendritic Cells

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Cells (2 × 10⁵ per well) were blocked on ice using 1 μL/mL Serobloc in blocking buffer (phosphate‐buffered saline [PBS], 5% heat inactivated normal rabbit serum (HI‐NRS), 1% bovine serum albumin (BSA), 2 mM sodium azide), and stained with anti‐CD11c‐APC (BD Pharmingen, Wokingham, Berks, UK; Cat# 550261), anti‐CD40‐FITC (BD Pharmingen, Cat# 553790), anti‐CD54‐PE (BD Pharmingen, Cat# 553253), anti‐CD80‐PE (BD Pharmingen, Cat# 553769), anti‐CD86‐PE (eBioscience, San Diego, California, Cat# 12‐0861‐81), anti‐H‐2K^k‐PE (BD Pharmingen, Cat# 553593), anti‐MHC class II‐PE (eBioscience, Cat# 12‐5321‐81) or anti‐SSEA‐1‐PE (eBioscience, Cat# 12‐8813‐71). For analysis of transcription factor expression, cells were permeabilized with 0.1% saponin for 5 minutes and stained using anti‐Nanog‐Alexa Fluor 488 (BD Pharmingen, Cat# 560278) or anti‐Oct4‐Alexa Fluor 647 (BD Pharmingen, Cat# 560329). Samples were washed three times in washing buffer (PBS, 1% FCS, 2 mM NaN₃), fixed in 2% formaldehyde/PBS, and analyzed using a FACSCalibur cytometer.

Horton C., Davies T.J., Lahiri P., Sachamitr P, & Fairchild P.J. (2019). Induced pluripotent stem cells reprogrammed from primary dendritic cells provide an abundant source of immunostimulatory dendritic cells for use in immunotherapy. Stem Cells (Dayton, Ohio), 38(1), 67-79.

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