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K2edta collection tubes

Manufactured by BD

K2EDTA collection tubes are used to collect and store blood samples for laboratory analysis. The tubes contain K2EDTA, which is an anticoagulant that prevents the blood from clotting, allowing for accurate testing.

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2 protocols using k2edta collection tubes

1

Multimodal Biospecimen Collection Protocol

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Total blood samples were collected into K2EDTA collection tubes (BD #367863). N = 7 of total blood samples were collected from healthy volunteers by a certified phlebotomist through venipuncture following IRB-FY2018-426 approved by Rice University. N = 10 human blood samples were purchased from ZenBio, shipped on collection day at 4°C and delivered next day. Demographic information of blood donors was provided by ZenBio. N = 6 plasma samples from diseased individuals (3 from Type 1 Diabetes (T1D) patients and 3 from Alzheimer’s disease (AD) patients) were acquired from BioIVT’s biorepository and shipped frozen. Animal total blood samples were purchased from Discovery Life Sciences, shipped on collection day at 4°C and delivered next day. Demographic information of all 23 subjects was summarized in Table S1.
Milk was sampled from whole milk bought from grocery. Fruit juiced were collected via peeling and squeezing the fruits. Centrifugation at 1500xg for 10min was performed and only supernatant was collected.
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2

High-Fat Tolerance Test Protocol

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On the day of the HFTT (D5), participants reported to the laboratory at 0700 h. Body weight was measured. They then lie for 5 min before insertion of a catheter into an antecubital vein. A fasting blood sample was obtained and fat oxidation was measured before consumption of a high-fat shake (mostly melted ice cream and heavy cream, approximately 14.8 kcal•kg -1 [0.8 g, 1.2 g, and 0.2 g•kg -1 BW of carbohydrate, fat, and protein, respectively]). Blood samples were collected over the next 6 h at 2, 3, 4 and 6 h postconsumption of the high-fat shake. All blood samples collected were transferred to K 2 EDTA collection tubes (BD), centrifuged at 2000g for 15 min at 4°C and then stored in -80°C freezer until later analysis. During HFTT, participants were asked to remain seated quietly reading, watching movies, and/or surfing the Internet. Participants were allowed to use the restroom.
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