From anti-coagulated whole blood DCs, CD4+ T cells and B cells were sorted following staining with monoclonal antibodies to anti-CD45 V450 (clone DO58–1283; BD Pharmingen), anti-CD14 FITC (clone M5E2; BD Pharmingen), HLA-DR PE (clone L243 (G46–6); BD Pharmingen), anti-CD20 ECD (clone B9E9; Beckman Coulter), anti-CD4 PerCP Cy5.5 (clone L200; BD Pharmingen), anti-CD123 APC (clone 7G3; BD Pharmingen), anti-CD11c APC (clone S-HCL-3; BD Pharmingen), anti-CD3 Alexa Fluor 700 (clone SP34.2; BD Pharmingen), anti-CD8 APC.H7 (clone SK1; BD Pharmingen). After staining and washes, stained PBMCs were sorted using the BD FACSAria II. Doublets were excluded from analysis by gating singlets in forward scatter-area (FSC-A) versus forward scatter-height (FSC-H) and side scatter-area (SSC-A) versus side scatter-height (SSC-H) analysis. After gating on lymphocytes (CD45+ followed by FSC-A vs. SSC-A), total B lymphocytes were defined and sorted as CD3- CD8- CD20+ populations. Dendritic cells were gated and sorted as CD3- CD20- CD14- CD11c+/CD123+ cells. Sorted populations were collected into tubes containing RNA Protect (Qiagen) for RNA isolation.
Anti cd123 apc clone 7g3
Manufactured by BD
Anti-CD123 APC (clone 7G3) is a fluorescently labeled antibody that binds to the CD123 antigen on the surface of cells. It is used for the identification and enumeration of CD123-expressing cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 alexa fluor 700 clone sp34.2, by BD (2 mentions)
Anti cd45 v450 clone do58 1283, by BD (2 mentions)
Hla dr pe clone l243 g46 6, by BD (2 mentions)
Anti cd20 ecd clone b9e9, by Beckman Coulter (2 mentions)
Anti cd11c apc clone s hcl 3, by BD (2 mentions)
Anti cd4 percp cy5.5 clone l200, by BD (2 mentions)
Anti cd14 fitc clone m5e2, by BD (2 mentions)
Anti cd8 apc h7 clone sk1, by BD (2 mentions)
Rnaprotect, by Qiagen (2 mentions)
Facsaria 2, by BD (2 mentions)
2 protocols using anti cd123 apc clone 7g3
1
Isolation of PBMCs Subpopulations
2
Isolation of PBMCs Subpopulations
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From anti-coagulated whole blood DCs, CD4+ T cells and B cells were sorted following staining with monoclonal antibodies to anti-CD45 V450 (clone DO58–1283; BD Pharmingen), anti-CD14 FITC (clone M5E2; BD Pharmingen), HLA-DR PE (clone L243 (G46–6); BD Pharmingen), anti-CD20 ECD (clone B9E9; Beckman Coulter), anti-CD4 PerCP Cy5.5 (clone L200; BD Pharmingen), anti-CD123 APC (clone 7G3; BD Pharmingen), anti-CD11c APC (clone S-HCL-3; BD Pharmingen), anti-CD3 Alexa Fluor 700 (clone SP34.2; BD Pharmingen), anti-CD8 APC.H7 (clone SK1; BD Pharmingen). After staining and washes, stained PBMCs were sorted using the BD FACSAria II. Doublets were excluded from analysis by gating singlets in forward scatter-area (FSC-A) versus forward scatter-height (FSC-H) and side scatter-area (SSC-A) versus side scatter-height (SSC-H) analysis. After gating on lymphocytes (CD45+ followed by FSC-A vs. SSC-A), total B lymphocytes were defined and sorted as CD3- CD8- CD20+ populations. Dendritic cells were gated and sorted as CD3- CD20- CD14- CD11c+/CD123+ cells. Sorted populations were collected into tubes containing RNA Protect (Qiagen) for RNA isolation.
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