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P nf κb antibody

Manufactured by Abcam
Sourced in United States

The P-NF-κB antibody is a laboratory reagent used to detect the p65 subunit of the NF-κB transcription factor in various experimental applications. NF-κB is a key regulator of immune and inflammatory responses.

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2 protocols using p nf κb antibody

1

Anti-inflammatory effects of STV-Na

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STV‐Na was provided by Key‐Pharma Biological Inc. LPS (Escherichia coli 0111: B4) was retrieved from Sigma Chemical Co. myeloperoxidase (MPO) antibody, F480 antibody, GAPDH antibody, NF‐κB antibody, p‐NF‐κB antibody and 4,6‐diamino‐2‐phenyl indole (DAPI) were obtained from Abcam. TLR4 antibody was obtained from Cell Signaling Technology. Inc. ROS fluorescent probes were purchased from Bestbio Biologicals Ltd. The anti‐rabbit immunoglobin G (IgG) antibody was purchased from Zhongshan Jinqiao Biological Co. ExCell Bio Co was the supplier of all enzyme‐linked immunosorbent assay (ELISA) kits. Wright–Giemsa staining solution, hematoxylin‐eosin (HE) Staining Kit, bicinchoninic acid (BCA) Protein Assay Kit, 5% bull serum albumin (BSA) Blocking Buffer, goat serum and Tris buffered saline containing 0.1%Tween 20 (TBST) were acquired from Solaibao Life Sciences Co. (Beijing, China).
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2

Astrocyte Isolation and IL-17 Signaling

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Fetal bovine serum (FBS) and DMEM/F12 medium were purchased from HyClone (Logan, UT, USA). Recombinant murine IL-17 was purchased from Peprotech (Rocky Hill, New Jersey, USA). Trypsin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Quantibody® Rat Cytokine Array 3 was purchased from RayBiotech (Inc., Guangzhou, China). A20, p-NF-κB antibody and β-actin rabbit polyclonal antibody were purchased from Abcam (Cambridge, Massachusetts, USA). High throughput sequencing of miRNA expression profiles was conducted by Huada gene Biotechnology (Shenzhen) Co., Ltd. in China; miRNA mimics and the negative control of miRNA mimics (miR-NC) were synthesized (Guangzhou iGeneBio Co., Ltd. in China). The construction and identification of pGL3-Promoter/WTA20 luciferase reporter plasmid were completed by Huada gene Biotechnology (Shenzhen) Co., Ltd. in China. Regarding the experimental animals and cells, astrocytes were isolated from the cerebral cortex of 0-2-day-old SD rats (Experimental Animal Center of Guangxi Medical University).
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