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2 protocols using ch3co2k

1

Comprehensive Chemical Reagent Procurement

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All chemicals (ACN, water, ethanol, hexane, BSA, Na2CO3, AlCl3, CH3CO2K, As2H6Mo3O12, trichloroanisole, Iron(II) chloride, Iron(III) chloride ferrozine, 2,2‐diphenyl‐1‐picrylhydrazyl, caffeic acid, p‐coumaric acid, naringin, quercetin, luteolin, apigenin, isorhamnetin‐3‐O‐glucoside, gallic acid, ascorbic acid, tannic acid, Folin Ciocalteu's reagent) unless otherwise stated, were purchased from Merck Life Science (Merck KGaA, Darmstadt, Germany).
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2

Comparative Analysis of Phyllanthus and Sonchus Medicinal Plants

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Phyllanthus niruri and Sonchus arvensis samples were collected from three institutions in Indonesia in March of 2018. (1) UPT Materia Medica Batu (the Province of Jawa Timur) is located at an altitude of ± 875 m above the sea level with a temperature of ±20-25 0 C. (2) Biopharmaca Cultivation Conservation Unit, Studies Center for Tropical Biopharmaca IPB Bogor (the Province of Jawa Barat) is situated at ±145-400 m above the sea level with a temperature of ±25-33 0 C. (3) B2P2TOOT Tawangmangu in Karanganyar (the Province of Jawa Tengah) is located at ±1200 m above the sea level with a temperature of ±22-31 0 C. These institutions were also responsible for determining the crude drugs of both plants. The chemicals used in the study were chloral hydrate (Merck), HCl (Merck), chloroform (Mallinckrodt), ethanol 96% (Merck), H2SO4 (Merck), NaNO2 (Merck), AlCl3 (Merck), NaOH (Merck), CH3CO2K (Merck), quercetin (Sigma), and demineralized water.
Meanwhile, the research equipment included analytical balance (Ohaus), oven (Memmert), moisture content balance (Mettler Toledo), desiccator (Duran), binocular microscopes (Olympus), furnace (Memmert), ultrasonic cleaner (Branson), spectrophotometer (Shimadzu), and laboratory glassware.
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