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2 protocols using 2 4 iodophenyl 3 4 nitrophenyl 5 phenyl 2h tetrazolium chloride

1

Quantitative Tetrazolium Reduction Assay

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Meat peptone and meat extract were purchased from Merck, Darmstadt, Germany. 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT), Tetracycline (≥98.0% (NT)) and Sephadex LH-20 were purchased from Sigma-Aldrich, St. Louis, MO, USA. All materials were used as received.
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2

Biochemical Reagents for Research

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Aminooxyacetic acid (AOAA), antimycin A, 7-azido-4-methylcoumarin, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), Coomassie blue R-250, S-(5′-adenosyl)-L-methionine chloride dihydrochloride (SAM), d-aminolevulinic acid (d-ALA), N,N-dimethyl-p-phenylendiamine-sulfate (DPD), 2-deoxyglucose, glutathione (GSH), homocysteine, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (INT), iron(III) chloride (FeCl3), lactic acid, L-cysteine, N-methylphenazonium methyl sulfate (PMS), nicotinamide adenine dinucleotide (NAD+), oligomycin, pyridoxal-5-phosphate (PLP), rotenone, sodium hydrosulfide hydrate (NaSH), zinc acetate (ZnAc) and trichloroacetic acid (TCA) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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